تأثير مستخلصات نبات الفيجل Ruta chalapensis L. على بعض أنواع البكتيريا المعزولة من التهاب الجروح

Ahmed Amrajaa  Abdulrraziq; Sami Mohammed  Salih

doi:10.54172/mjsc.v33i3.245

Authors

Ahmed Amrajaa Abdulrraziq Department of Biology, Faculty of Education, Omar Al-Mukhtar University, Al-Bayda, Libya
Sami Mohammed Salih Department of Biology, Faculty of Education, Omar Al-Mukhtar University, Al-Bayda, Libya

DOI:

https://doi.org/10.54172/mjsc.v33i3.245

Keywords:

Ruta chalapensis L, Bacteria, wound inflammation

Abstract

Bacteria are among the main causes of infection and wound infections because of toxins that may delay the healing process, Which has become resistant to most antibiotics and has been directed towards medicinal plants as alternative treatments. This study, which was conducted in the Plant Pathology Laboratory of the Plant Protection Department of the Faculty of Agriculture, investigated the inhibitory effect of the water extract, ethanol and acetone of Ruta chalepensis L. using several concentrations 50, 100, 150 and 200 mg/ml against four types of bacteria (Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Streptococcus pyogenes) isolated from wound inflammation to Al-Jalaa Hospital in Benghazi-Libya by a sensitivity test in the form of Disk diffusion method. then compared with Gentamicin. The results showed that the concentration of 200 mg/ml for all Ruta chalepensis L. extract was the most effective, and the concentration of 50 mg/ml did not show any inhibitory effect on all types of bacteria studied, Streptococcus pyogenes was the most sensitive of all concentrations of extracts with diameters of between (1.3 - 14.3)mm, While Pseudomonas aeruginosa was the most resistant to concentrations of all extracts and antibiotics, The results also showed that the ethanol and acetone extracts were superior to the Gentamicin on all bacteria except Escherichia coli.

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References

Abdulkadir, M. and Waliyu, S. (2012). Screening and Isolation of the Soil Bacteria for Ability to Produce Antibiotics. European Journal of Applied Sciences, 4, 211-215.

Abu-Mejdad, N. M. J. (2009). Antimicrobial activity of Citrullus colocunthis L., Ruta Chalepensis L. and Curcuma longa Roxb. Extract in vitro. Journal of Univesity of Thi-Qar. 4 (4), 1-8.

Abu-shanab, B. Adwan, G. Abu-safiya, D. Jarrar, N. and Adwan, K. (2004). Antibacterial activity of some plant extract unit izedinpopular medicine in plant. Turkish Journal of Biology, 28, 99- 105.

Abu-shanab, B. Adwan, G. Adwan, K. and Abu-shanab, F. (2008). Efficacy of Aqueous and Ethanol Extracts of Some Palestinian Medicinal Plants for Potential Antibacterial Activity. Journal The Islamic University,16(2) ,77-86.

Aghazadeh, M. Hojabri, Z. Mahdian, R. Nahaei, M. R. Rahmati, M.Hojabri, T. Pirzadeh, T. and Pajand, O. (2014). Role of efflux pumps: MexABOprM and MexXY (-OprA), AmpC cephalosporinase and OprD porin in nonmetallo-β-lactamase producing Pseudomonas aeruginosa isolated from cystic fibrosis and burn patients. Infection, Genetics and Evolution. 24, 187-192. DOI: https://doi.org/10.1016/j.meegid.2014.03.018

Ahmed, S. A. Mahmoud, S. S. and Alkadim, L. S. (2008). Study of antimicrobial activity of Salvia officinalis extracts in the growth of pathogenic bacteria. Iraqi Journal Of Biotechnology, 7(1), 51-63.

Ali-Shtayeh, M. S. and Abu Ghdeib, S. I. (1999). Antifungal activity of plant extractsagainstdermatophytes. Mycoses, 42,665–672. DOI: https://doi.org/10.1046/j.1439-0507.1999.00499.x

Al-Salehy, Sh. H. Al-Hayany, A. M. Al-Mawla, R. M. (2009). Discovering secondary metabolism products and mineral elements in Ruta chalepensis. Journal of Research Diyala Humanity , (36), 69-82.

Al-Sokari, S. S. and El-Sheikha, A. F. (2015). In vitro antimicrobial activity of crude extracts of some medicinal plants from Al-Baha region in SaudiArabia. , 3(1-2), 74-78. DOI: https://doi.org/10.11648/j.jfns.s.2015030102.24

Bhattacharje, I. Chatterjee, S. k. and Chanadro, G. (2006). Antibacterial potentiality of Argenome mexicana solvent extract against some pathogenic bacteria. Mem.Inst.os-waldo cruz., Rio Dejaneiro.101(6), 642-648 DOI: https://doi.org/10.1590/S0074-02762006000600011

Boberek, J. M. Stach, J. Good, L. (2010). Genetic evidence for inhibition of bacterial division protein FtsZ by berberine. www. Plosone.org,.5 (10) e13754. DOI: https://doi.org/10.1371/journal.pone.0013745

Bouzidi, M. A. Latrèche, A. Attaoui, I. Benabderrahmane, M. Mehdadi, Z. and Benyahia, M. (2012). Antibacterial effect of the essential oils extracted from Ruta chalepensis L. and Ruta montana (L.) L. Journal of Life Sciences, (6), 898-902.

Dale, R. M. K. Schnell, G. and Wong, J. P. (2004). Therapeutic efficacy of "Nubiotics" against burn wound infection by Pseudomonas aeruginosa. Antimicrob Agents Chemother, 48(8), 2918-2923. DOI: https://doi.org/10.1128/AAC.48.8.2918-2923.2004

Fakhfakh, N. Zouari, S. Zouari, M., Loussayef, C. and Zouari, N. (2012). Chemical composition of volatile compounds and antioxidant activities of essential oil, aqueous and ethanol extracts of wild Tunisian Ruta chalepensis L. (Rutaceae). Journal of Medicinal Plants Research, , 6, 593–600. DOI: https://doi.org/10.5897/JMPR11.1121

Forbes, B. A. Sahm, D. F. and Weissfeld, A. S. (2007). Bailey and Scott's Diagnostic Microbiology. 12th ed. Mosby elservier. PP. 897.

Gordon, R. J. and Lowy, F. D. (2008). Pathogenesis of methicillin resistant Staphylococcus aureus infection. Clinical Infectious Diseases, 46, S350- S359. DOI: https://doi.org/10.1086/533591

Haddouchi, F. Chaouche, T. M. Zaouali, Y. Ksouri, R. Attou, A. Benmansour, A. (2013). Chemical compositionand antimicrobial activity of the essential oils from four Ruta species growingin Algeria. Food Chemistry, 141, 253–258. DOI: https://doi.org/10.1016/j.foodchem.2013.03.007

Hosseinzadeh, H. Bazzaz, B. S. F. and Sadati, M. M. (2006). In vitro evaluation of methyl xanthines and some antibiotics: Interaction against Staphylococcus aureus and Pseudomonas aeruginosa. Iranian Biomedical Journal, 10,163-167.

Hussien, I. A. Habib, K. A. and Jassim, K. A. (2012). Bacterial Colonization of Burn Wounds. Baghdad Science Journal,9(4). DOI: https://doi.org/10.21123/bsj.9.4.623-631

Kahlmeter, G. Menday, P. (2003). Cross-resistance and associated resistance in 2478 Escherichia coli isolates from the Pan-European ECO.SENS project surveying the antimicrobial susceptibility of pathogens from uncomplicated urinary tract infections. Journal of Antimicrobial Chemotherapy, 52, p128-131. DOI: https://doi.org/10.1093/jac/dkg280

Kasimala, M. Tukue. M. and Ermias. R. (2014). Phytochemical screening and antibacterial activity of two common terrestrial medicinal plants Ruta Chalapensis and Rumex nervosas. Bali Medical Journal, 3( 3), 116-121. DOI: https://doi.org/10.15562/bmj.v3i3.86

Kuzovkina, I., Al-terman, I. and Schneider, B. (2004). Specific accumulation and revised structures of acridone alkaloid glucosides in the tips of transformed roots of Ruta graveolens. Phytochemistry, 65, 1095-1100. DOI: https://doi.org/10.1016/j.phytochem.2004.03.003

Lauk, L., Mangano, K., Rapisarda, A. (2004). Protection against murine endotoemia by treatment with Ruta chalepensis L.; a plant with anti-inflammatory properties. Journal of Ethnopharmacology, 90, 267 - 272. DOI: https://doi.org/10.1016/j.jep.2003.10.004

MacFaddin, J. F. (2000). Biochemical Tests for Identification of Medical Bacteria. 3rd edn., Williams and Wilkins, Philadelphia, P. A: 555-565.

Magnet, S. and Blanchard, J. S. (2005). Molecular insights into aminoglycoside action and resistance. Chemical Reviews, 105 (2), 477–498. DOI: https://doi.org/10.1021/cr0301088

Mohammed, M. J. (2006). Study the Biological Effect of Alkaloids and Phenols Separated from Ruta graveolens L. (fruits) on Growth of Some Gram Positive and Negative Bacteria. Tikrit Journal of Pure Science, 11 (1), 183-186.

Naas, T., Zerbib, M., Girlich, D. and Nordmann, P. (2003). Integration of a transposon Tn1-encoded inhibitor-resistant-lactamase gene, blaTEM-67 from Proteus mirabilis, into the Escherichia coli chromosome. J. Antimicrobial agents and Chemotherapy. 47 (1), 19-26. DOI: https://doi.org/10.1128/AAC.47.1.19-26.2003

Parekh, J. and Chanda, S. (2006). In vitro antimicrobial activities of extracts of Launaea procumbens Roxb. (Labiateae), Vitis vinifera L. (Vitaceae) and Cyperus rotundus L. (Cyperaceae). African Journal of Biomedical Research , 9, 89-93. DOI: https://doi.org/10.4314/ajbr.v9i2.48780

Parekh, J. and Chanda, S. (2007). In vitro antimicrobial activity and phytochemical analysis of some Indianmedicinal plants. Turkish Journal of Biology , 31: 53 – 58.

Sher, A. (2009). Antimicrobial activity of natural products from medicinal plants. Gomal Journal of Medical Sciences..7(1):72-78.

Singh, S. and Verma, S. S. K. (2011). Antibacterial properties of alkaloid rich fractions obtained from various parts of Prosopis juliflora. International Journal of Pharma Sciences and Research,2 (3),114-120.

Stanzani, M., Tumietto, F. Giannini, M. B. Bianchi, G. Nanetti, A. Vianelli, N. Arpinati, M. Giovannini, M. Bonifazi, F. Bandini, G. and Baccarani, M. (2007). Successful treatment of multi-resistant Pseudomonas aeruginosa osteomyelitis after allogeneic bone marrow transplantation with a combination of colistin and tigecycline. Journal of Medical Microbiology. 56, 1692-1695. DOI: https://doi.org/10.1099/jmm.0.47286-0

Tendencia, E. A. (2004). Disk diffusion method. In Laboratory manual of standardized methods for antimicrobial sensitivity tests for bacteria isolated from aquatic animals and environment, pp. 13-29. Tigbauan, Iloilo, Philippines: Aquaculture Department, Southeast Asian Fisheries Development Center.

Zangana, P. M .M. (2004). Isolation And Identification Of Aerobic Bacteria Before And After Sterilization In The Theatres And Wards Of Tikrit Teaching Hospital. Degree of Masters ,College of Education ,Women University of Tikrit