Determining the Prevalence of Bacterial Vaginosis and their Patterns of Susceptibility to Antibiotics among Benghazi Women, Libya


  • Noor alhooda M. Alawkally Department of Medical laboratory, Higher Institute of Science and Technology, Suluq, Libya
  • Hamza K. Ibrahim Department of pharmacy, Higher Institute of medical Technology, Bani Waleed, Libya
  • Nessren F. Mousa Department of Statistics, Faculty of arts and sciences, University of Benghazi, Libya
  • Mareei. A. Ali Department of Medical laboratory, Higher Institute of Science and Technology, Suluq, Libya
  • Abdlmanam S. Fakron Department of Microbiology, Science Faculty, Omar Al Mukhtar University, Libya
  • Nesrine M. Al-awkally Department of Surgery, Alhaowari hospital, Benghazi, Libya
  • Muftah A. Nasib Department of Microbiology, Faculty of Science, Omar Al Mukhtar University, Libya
  • Alreda M. Al-Awkally Central Pharmacy, Ministry of health, Darna, Libya
  • Fathia M. SenossI Department of Zoology, Faculty of Art and Science, Benghazi University, Libya



Al saleem medical laboratory, Augmentin, Ceftriaxon, E. coli, Strep pneumoniae


Vaginal discharge in women is occasionally caused by aerobic bacterial organisms. The study aimed to determine the etiology of female vaginosis and their antibiotic sensitivity pattern. HV culture results, age, and sex of all female patients with suspected bacterial vaginosis were collected. High vaginal swabs were inoculated into MacConkey agar, 5% blood agar, and chocolate agar and then incubated at 37°C in the presence of 5% CO2 for 24-48 h. The antimicrobial susceptibility testing was performed by the disk diffusion method. Ten different antibiotic discs were used: Amikacin, Augmentin, Ceftazidime, Ceftriaxone, Ciprofloxacin, Gentamicin, Levofloxacin, Meropenem, Septrin, and Clindamycin. After 24 hours, zones were measured in mm, and zone interpretations were in accordance with the National Committee for Clinical Laboratory Standards criteria guidelines. A total of 215 females were included in the study, the incidence of bacterial vaginosis was 18.6% (40/215). Females between 33 and 45 years old had a somewhat high prevalence (19/40:47.5%) of bacterial vaginosis. The most frequent isolates were 45% (18/40) Escherichia coli followed by 15% (6/40) Strep pneumonia. The in vitro susceptibility tests of the most common isolates showed high resistance levels to commonly used antibiotics such as Augmentin and Gentamycin. Whereas highly sensitive rates were observed for Ceftriaxone 70%, followed by Ciprofloxacin 57.5%. Ceftriaxone and Ciprofloxacin showed the best antibiotic sensitivity. Additional studies are necessary to recognize those bacterial species that cause vaginal infections and determine the susceptibility of those species to recently used antibiotics.


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Abera, B., & Kibret, M. (2011). Bacteriology and antimicrobial susceptibility of otitis media at dessie regional health research laboratory, Ethiopia. Ethiopian Journal of Health Development, 25(2), 161-167.

Adnan, E. K., Aymen H, E. E., & Nasser Yehia A, A. (2008). Streptococcus pneumoniae as a cause of early onset neonatal sepsis: first report from Kuwait.

Agarwal, S K et al. (2900). “Antifungal activity of anthraquinone derivatives from Rheum emodi.” Journal of ethnopharmacology vol. 72,1-2 43-6. DOI:

Alawkally, Noor Alhooda & Nag, Miftah & Ali, Maree & Al-Awkally, Nesrine & Al-Awkally, Abeer & Al-Awkally, Alreda. (2022). Antibiotic sensitivity of Escherichia coli isolated from different clinical specimens of patient human in Al-jala hospital-Benghazi Libya. International Journal of Current Research in Science Engineering & Technology. 8. Pages: 509-517

Bibi Ayesha٫. Sana Jabeen., Muhammad Ismail., Sayyed Salman., Sana Ullah, Zeeshan Niaz., Tauseef Ahmad. (2014). Isolation, Identification And Antibiotic Susceptibility Testing Of Microorganisms From Female Patients Of Ayub Medical Complex Through High Vaginal Swab. Sci.Int.(Lahore),26(4),1581-1586, ISSN 1013-5316; Coden: Sinte 8

Boris, S., Suárez, J. E., Vázquez, F., & Barbés, C. (1998). Adherence of human vaginal lactobacilli to vaginal epithelial cells and interaction with uropathogens. Infection and immunity, 66(5), 1985-1989. DOI:

Das, C., Pattnaik, P., & Sahoo, P. (1996). Prevalence of vaginal infection in preterm labour. Antiseptic, 93(4), 140-143.

Donders, G., Van Calsteren, K., Bellen, G., Reybrouck, R., Van den Bosch, T., Riphagen, I., & Van Lierde, S. (2009). Predictive value for preterm birth of abnormal vaginal flora, bacterial vaginosis and aerobic vaginitis during the first trimester of pregnancy. BJOG: An International Journal of Obstetrics & Gynaecology, 116(10), 1315-1324. DOI:

Farrar, W. E. (1985). Antibiotic resistance in developing countries. The Journal of infectious diseases, 152(6), 1103-1106. DOI:

Fakron, A., Alawkally, N., El- warred, S., Aldouakali Ali , M., El-amari , M., Awkally, A., Soutiyah, M., Suliman, N. and Ibrahim, H. (2022). Risk Factors for Ciprofloxacin and Gentamycin Resistance among Gram Positive and Gram Negative Bacteria Isolated from Community-Acquired Urinary Tract Infections in Benghazi city. Scientific Journal for the Faculty of Science-Sirte University. 2, 1 (Apr. 2022), 76-87. DOI:

Forsum, U., Holst, E., Larsson, P.-G., Vasquez, A., Jakobsson, T., & Mattsby‐Baltzer, I. (2005). Bacterial vaginosis–a microbiological and immunological enigma. Apmis, 113(2), 81-90. DOI:

Galea, G., Abela, R., Deguara, C., Cuschieri, P., & Brincat, M. P. (2014). Puerperal Streptococcus pneumoniae endometritis: a case report and literature review.

Go, V. F., Quan, V. M., Celentano, D. D., Moulton, L. H., & Zenilman, J. M. (2006). Prevalence and risk factors for reproductive tract infections among women in rural Vietnam. Southeast Asian Journal of Tropical Medicine and Public Health, 37(1), 185.

Hutchison, C., Kenney, A., & Eykyn, S. (1984). Maternal and neonatal death due to pneumococcal infection. Obstetrics and gynecology, 63(1), 130-131.

Noor-alhooda Milood Al-awkally, Maree DokallyAli, ReedaMiloud Al-awkally, Abeer Miloud AL-awkally, Fowziya M. Ali and Ahmed Abouserwel. (2019). Antibiotic Susceptibility and Resistant Pattern of Isolates of Pseudomonas aeruginosa recovered from Infected Swabs, Abscess, Burn, Medical Tips and Blood from Patients at 4 Geographical Locations in Libya (Al- Bayda, Shahat, Derna and Benghazi). Int.J.Curr.Microbiol.App.Sci. 8(10): 143-149. DOI:

Martin, R., Soberon, N., Vazquez, F., & Suárez, J. E. (2008). Vaginal microbiota: composition, protective role, associated pathologies, and therapeutic perspectives. Enfermedades Infecciosas y Microbiología Clínica, 26(3), 160-167. DOI:

Mohammed, M. A., Alnour, T. M., Shakurfo, O. M., & Aburass, M. M. (2016). Prevalence and antimicrobial resistance pattern of bacterial strains isolated from patients with urinary tract infection in Messalata Central Hospital, Libya. Asian Pacific journal of tropical medicine, 9(8), 771-776. DOI:

Mumtaz, S., Ahmad, M., Aftab, I., Akhtar, N., ul Hassan, M., & Hamid, A. (2008). Aerobic vaginal pathogens and their sensitivity pattern. J Ayub Med Coll Abbottabad, 20(1), 113-117.

Mylonas, I., & Friese, K. (2007). Genital discharge in women. MMW Fortschritte der Medizin, 149(35-36), 42-46; quiz 47. DOI:

Orish, V. N., Ofori-Amoah, J., François, M., Silverius, B. K., & Mensah, E. K. (2016). Microbial and antibiotic sensitivity pattern of high vaginal swab culture results in sekondi-takoradi metropolis of the western region of ghana: retrospective study. European Journal of Clinical and Biomedical Sciences, 2(5), 45-50.

Polat IH, Marin S, Ríos J, et al. (2021). Exploratory and confirmatory analysis to investigate the presence of vaginal metabolome expression of microbial invasion of the amniotic cavity in women with preterm labor using high-performance liquid chromatography. Am J Obstet Gynecol ;224:90.e1-9. DOI:

Petti, C. A., Ignatius Ou, S.-H., & Sexton, D. J. (2002). Acute terminal ileitis associated with pneumococcal bacteremia: case report and review of pneumococcal gastrointestinal diseases. Clinical Infectious Diseases, 34(10), e50-e53. DOI:

Ravishankar, N., & Prakash, M. (2017). Antibiogram of bacterial isolates from high vaginal swabs of pregnant women from tertiary care hospital in Puducherry. India IJCMAS, 6, 964-972. DOI:

Singh, S., Swain, S., Das, L., Das, P. C., & Sahoo, S. (2016). Isolation and characterization of organisms in high vaginal swab culture in preterm pregnancy (28-37 week). International Journal of Reproduction, Contraception, Obstetrics and Gynecology, 5(11), 3853-3859. DOI:

Spinillo, A., Bernuzzi, A. M., Cevini, C., Gulminetti, R., Luzi, S., & De Santolo, A. (1997). The relationship of bacterial vaginosis, Candida and Trichomonas infection to symptomatic vaginitis in postmenopausal women attending a vaginitis clinic. Maturitas, 27(3), 253-260. DOI:

Szöke, I., Pascu, C., Nagy, E., LJUNG, A., & Wadstrom, T. (1996). Binding of extracellular matrix proteins to the surface of anaerobic bacteria. Journal of medical microbiology, 45(5), 338-343. DOI:

Tripathi, G. (2003). Potentials Of Living Resources. Discovery Publishing House.

Ullah, Sana. (2014). Isolation, Identification and Antibiotic Susceptibility Testing of Microorganisms from Female Patients of Ayub Medical Complex Through High Vaginal Swab. Science International. 26. 1581-1587.

Watts DH, Fazzari M, Minkoff H, Hillier SL, Sha B, Glesby M, Levine AM, Burk R, Palefsky JM, Moxley M, Ahdieh-Grant L, Strickler HD. (2005). Effects of bacterial vaginosis and other genital infections on the natural history of human papillomavirus infection in HIV-1-infected and high-risk HIV-1-uninfected women. J Infect Dis. 2005 Apr 1;191(7):1129-39. doi: 10.1086/427777. Epub 2005 Feb 21. Erratum in: J Infect Dis. Sep 1;192(5):943. Fazarri, Melissa [corrected to Fazzari, Melissa]. PMID: 15747249. DOI:

Workowski, K. A., & Berman, S. M. (2010). Sexually transmitted diseases treatment guidelines.




How to Cite

Alawkally, N. alhooda M., Ibrahim, H. K., Mousa , N. F., Ali, M. A., Fakron , A. S., Al-awkally, N. M., Nasib, M. A., Al-Awkally, A. M., & SenossI , F. M. (2022). Determining the Prevalence of Bacterial Vaginosis and their Patterns of Susceptibility to Antibiotics among Benghazi Women, Libya. Al-Mukhtar Journal of Sciences, 37(2), 127–137.



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