Efficacy of Aqueous Extracts of some Libyan Medicinal Plants Against Sclerotinia sclerotiorum In Vitro

Authors

  • Nisreen M. Hypa Department of Plant Protection, Faculty of Agriculture, Omar Almukhtar University, Elbeida-Libya
  • Zahra I. El-Gali Department Plant Protection, Faculty of Agriculture Omer Al-Mukhtar University, Libya.

DOI:

https://doi.org/10.54172/mjsc.v37i4.952

Keywords:

Medical plants, Aqueous extracts, Radial growth, Sclerotinia sclerotiorum, Libya

Abstract

The study was conducted to test the effect of aqueous extracts of the leaves of ten medicinal plants growing naturally in Al-Jabal Al-Akhdar region – northeast Libya, which include: Alhagi (Alhagi camelrum), Mugwort (Artimesia herba-alba), Everlasting (Helichrysum stoechas), Chamomile (Anthemis noblis), Stinkweed (Peganum harmala), Nettle (Urtica dioica), Rosemary (Rosmarinus officinalis), Rue (Ruta graveolens), Geranium (Pelargonium graveolens) and Castor (Risinus communis) using poisoned plate method in PSA medium against Sclerotinia sclerotiorum caused seeds’ rot and seedlings damping-off. The results showed a significant difference in inhibition effectiveness in all extracts against the tested fungus. Among all plants, H. stoechas was the most effective (88.4%), followed by U. dioica (79.1%), then P. harmala (78.3%). Chemical detections showed that the extracts of H. stoechas, P. harmala, and U. dioica contained some antioxidants:  phenols, flavonoids, and tannins. Based on these results application of plant extracts can be considered a beneficial strategy for controlling fungal plant diseases.

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References

اليحيى، سامي بن عبدالعزيز. (2007). دور المستخلصات النباتية الطبيعية في مقاومة الفطريات المسببة للأمراض النباتية. رسالة ماجستير. جامعة الملك سعود. السعودية. 154 صفحة.

دراسة وتقييم الغطاء النباتي الطبيعي بمنطقة الجبل الأخضر – التقرير النهائي. (2005). جامعة عمر المختار. 946 صفحة.

زوين، قيس كاظم وداود، حارث سمير. (2015). تأثير بعض المُستخلصات النباتية وعامل المُقاومة الأحيائية Trichoderma harzianum في التعفن الأبيض على الباذنجان المُتسبب عن الفطر Sclerotinia sclerotiorum. مجلة جامعة تكريت للعلوم الزراعية، 15(1): 138- 153.

لاربد، أمينة امبارك (2013). بدائل آمنة لوقاية البصل (Allium cepa) المخزون من الإصابة بالفطر Botrytis cinerea. رسالة ماجستير- كلية الزراعة- جامعة عمر المختار- 110 صفحة.

منصور، منصور سالم؛ سعيد، محمد علي؛ محمد، نوارة علي، وابراهيم، نجوى عبدالستار. (2012). استخدام بعض المُستخلصات النباتية في مُكافحة بعض فطريات أعفان جذور الحمص. المجلة الليبية لوقاية النبات، 2(1): 77- 93.

Akrout, A., Mighri, H., Krid, M., Thabet, F., Turki, H., El-Jani, H., & Neffati, M. (2012). Chemical composition and antioxidant activity of aqueous extracts of some wild medicinal plants in southern Tunisia. International Journal of Life Science and Medical Science, 2(1), 1-4. DOI: https://doi.org/10.5963/LSMR0201001

Amadi, J., Salami, S., & Eze, C. (2010). Antifungal properties and phytochemical screening of extracts of African basil (Ocimum gratissimum L.). Agriculture and Biology Journal of North America, 1(2), 163-166.

Apsara, S. G., Dhananjaya, V.K., Mallesha, H. and Ravikumar, K.R. (2012). Biological control of postharvest fungal pathogens of sweet oranges by Plumeria latex. Asian J. Plant Sci. and Res, 2(5), 613-619.

Arif, T., Bhosale, J., Kumar, N., Mandal, T., Bendre, R., Lavekar, G., & Dabur, R. (2009). Natural products–antifungal agents derived from plants. Journal of Asian natural products research, 11(7), 621-638. DOI: https://doi.org/10.1080/10286020902942350

Batchelder, T. (2004). The chemical anthropology of antimicrobial plants. Townsend Letter for Doctors and Patients(252), 130-134.

Bigović, D. J., Stević, T. R., Janković, T. R., Noveski, N. B., Radanović, D. S., Pljevljakušić, D. S., & Djurić, Z. R. (2017). Antimicrobial activity of Helichrysum plicatum DC. Hemijska industrija, 71(4), 337-342. DOI: https://doi.org/10.2298/HEMIND160118044B

Cushnie, T. T., & Lamb, A. J. (2005). Antimicrobial activity of flavonoids. International journal of antimicrobial agents, 26(5), 343-356. DOI: https://doi.org/10.1016/j.ijantimicag.2005.09.002

Datta, B., Das, A., & Ghosh, S. (2004). Fungal antagonists of some plant pathogens. Mycopathology, 1, 15-17. DOI: https://doi.org/10.1081/E-EPCS-120019942

Dellavalle, P. D., Cabrera, A., Alem, D., Larrañaga, P., Ferreira, F., & Dalla Rizza, M. (2011). Antifungal activity of medicinal plant extracts against phytopathogenic fungus Alternaria spp. Chilean journal of agricultural research, 71(2), 231-239 DOI: https://doi.org/10.4067/S0718-58392011000200008

Dixit, S.N., Tripathy, S.C. & Upadhyey, R.R. (1974). The antifungal substances of role flower (Rose indica). Econ. Bot., 30: 371-374. DOI: https://doi.org/10.1007/BF02904658

Edeoga, H. O., Okwu, D., & Mbaebie, B. (2005). Phytochemical constituents of some Nigerian medicinal plants. African journal of biotechnology, 4(7), 685-688. DOI: https://doi.org/10.5897/AJB2005.000-3127

Eloff, J., Mdee, L., & Masoko, P. (2007). Invasive and weedy species can be used as a source of antifungal compounds to control plant fungal pathogens. South African Journal of Botany, 73(2), 287. DOI: https://doi.org/10.1016/j.sajb.2007.02.041

Ewansiha, J., Garba, S., Galadima, M., Daniyan, S., & Busari, M. (2016). Therapeutic potency of Citrus limon (L) Burm. F.(lemon) peel extract against some disease causing microorganisms. International Journal of Research Studies in Biosciences, 4(11), 30-39. DOI: https://doi.org/10.20431/2349-0365.0411006

Farooq, M. A., Iqbal, U., Iqbal, S. M., Afzal, R., & Rasool, A. (2010). In-vitro evaluation of different plant extracts on mycelial growth of Sclerotium rolfsii the cause of root rot of sugar beet. Mycopath, 8(2), 81-84.

Masih, H., Peter, J., & Tripathi, P. (2014). A comparative evaluation of antifungal activity of medicinal plant extracts and chemical fungicides against four plant pathogens. International Journal of Current Microbiology and Applied Sciences, 3(5), 97-109.

Moss, S. (1986).The biology of marine fungi. Cambridge University Press, Cambridge, UK., ISBN: 0-521- 30899- 2, pp: 76.

Rani, P., Sudheer, S., & Devanand, P. (2006). Herbicidal potential of Breynia retusa leaf extract on Calotropis gigantea, Parthenium hysterophorus, Datura metal and Tridax procumbens. Allelopathy Journal, 17(1), 65-79.

Roussis, V., Tsoukatou, M., Chinou, I. B., & Harvala, C. (2002). Composition and antibacterial activity of the essential oils of two Helichrysum stoechas varieties growing in the Island of Crete. Journal of Essential Oil Research, 14(6), 459-461. DOI: https://doi.org/10.1080/10412905.2002.9699920

Saravanakumar, A., Venkateshwaran, K., Vanitha, J., Ganesh, M., Vasudevan, M., & Sivakumar, T. (2009). Evaluation of antibacterial activity, phenol and flavonoid contents of Thespesia populnea flower extracts. Pakistan Journal of Pharmaceutical Sciences, 22, 282- 286

Schwan-Estrada, K., & Stangarlin, J. (2005). Extracts and essential oils of medicinal plants in the resistance induction against plant pathogens. Piracicaba: FEALQ, 125-138.

Sobhy, E., & El-Feky, S. (2007). Chemical constituents and antimicrobial activity of Helichrysum stoechas. Asian Journal of plant sciences, 6(4), 692- 695 DOI: https://doi.org/10.3923/ajps.2007.692.695

Tao, Y., Zeng, F., Ho, H., Wei, J., Wu, Y., Yang, L., & He, Y. (2011). Pythium vexans causing stem rot of Dendrobium in Yunnan Province, China. Journal of Phytopathology, 159(4), 255-259. DOI: https://doi.org/10.1111/j.1439-0434.2010.01756.x

Tomás-Barberán, F., Iniesta-Sanmartín, E., Tomás-Lorente, F., & Rumbero, A. (1990). Antimicrobial phenolic compounds from three Spanish Helichrysum species. Phytochemistry, 29(4), 1093-1095. DOI: https://doi.org/10.1016/0031-9422(90)85410-H

Tsuchiya, H., Sato, M., Miyazaki, T., Fujiwara, S., Tanigaki, S., Ohyama, M., Tanaka, T., & Iinuma, M. (1996). Comparative study on the antibacterial activity of phytochemical flavanones against methicillin-resistant Staphylococcus aureus. Journal of Ethnopharmacology, 50(1), 27-34. DOI: https://doi.org/10.1016/0378-8741(96)85514-0

Published

2022-12-31

How to Cite

Hypa, N. M., & El-Gali, Z. I. (2022). Efficacy of Aqueous Extracts of some Libyan Medicinal Plants Against Sclerotinia sclerotiorum In Vitro . Al-Mukhtar Journal of Sciences, 37(4), 438–446. https://doi.org/10.54172/mjsc.v37i4.952

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