Effect of Sucrose Concentrations and Incubation Periods on in Vitro Rooting of Moris Pineapple (Ananas comosus)
DOI:
https://doi.org/10.54172/mjsc.v34i4.145Keywords:
Sucrose, Incubation Periods, In Vitro Rooting, Moris Pineapple, Ananas comosusAbstract
The effect of 6 sucrose concentrations (5, 10, 15, 20, 25, 30 g/l) over 4 incubation periods (30, 45, 60, 75 days) on in vitro rooting of Moris pineapple cultured in liquid half strength MS medium enriched with IBA at 2.0 mg/l was investigated. At all incubation periods, all shoots in medium enriched with sucrose at 5 g/l failed to root, and no roots formed within the first 30 days in medium enriched with sucrose at 10 g/l. After 30 days of incubation, the highest rooting percentage (66 %), tallest plantlets (23 mm tall), highest (3.4 roots) and longest (5.3 mm) root per shoot were obtained in medium enriched with sucrose at 25, 10, 15, 15 g/l respectively, while after 45 days, the highest of all rooting aspects (75 %, 32.3 mm tall, 3.7 roots, 7 mm long), were obtained in medium enriched with sucrose at 15 g/l. After 60 days, the highest rooting percentage (91.7 %) and tallest plantlets (36.7 mm tall) were obtained in medium enriched with sucrose at 20 g/l while highest roots per shoot (3.7 roots) and longest root (10.7 mm) were obtained in medium enriched with sucrose at 15 g/l. After 75 days, all shoots rooted (100 %) in medium enriched with sucrose at 10 and 20 g/l, while sucrose at 25 g/l resulted in tallest plantlets (46.3 mm tall) and at 20 g/l resulted in highest (4.7 roots) and longest roots (27.3 mm). At each incubation period, there was a different optimum sucrose enrichment for different rooting parameters.
Downloads
References
Almeida, W. A. B. D., Santana, G. S., Rodriguez, A. P., & Costa, M. A. P. D. C. (2002). Optimization of a protocol for the micropropagation of pineapple. Revista Brasileira de Fruticultura, 24(2), 296-300. DOI: https://doi.org/10.1590/S0100-29452002000200005
Amin, M., Rahman, M., Rahman, K., Ahmed, R., Hossain, M., & Ahmed, M. (2005). Large Scale Plant Regeneration in vitro from Leaf Derived Callus Cultures of Pineapple [Ammas comosus (L.) Merr. cv. Giant Kew]. DOI: https://doi.org/10.3923/ijb.2005.128.132
Aydieh, A., Ibrahim, M., & Ibrahim, A. (2000). In vitro propagation and fruiting of pineapple. Egyptian Journal of Horticulture, 27(3), 289-304.
Be, L., & Debergh, P. (2006). Potential low-cost micropropagation of pineapple (Ananas comosus). South African Journal of Botany, 72(2), 191-194. DOI: https://doi.org/10.1016/j.sajb.2005.07.002
Bhatia, P., & Ashwath, N. (2000). Development of a rapid method for micropropagation of a new pineapple [Ananas comosus (l.) Murr.] clone,'Yeppoon gold'. International Symposium on Tropical and Subtropical Fruits 575.
Dal Vesco, L. L., de Almeida Pinto, A., Zaffari, G. R., Nodari, R. O., dos Reis, M. S., & Guerra, M. P. (2001). Improving pineapple micropropagation protocol through explant size and medium composition manipulation. Fruits, 56(3), 143-154. DOI: https://doi.org/10.1051/fruits:2001100
Danso, K., Ayeh, K., Oduro, V., Amiteye, S., & Amoatey, H. (2008). Effect of 6-benzylaminopurine and naphthalene acetic acid on in vitro production of MD2 pineapple planting materials. World Applied Sciences Journal, 3(4), 614-619.
De Almeida, W., De Matos, A., & Souza, A. d. S. (1997). Effects of benzylaminopurine (BAP) on in vitro proliferation of pineapple (Ananas comosus (L.) Merr.). II International Pineapple Symposium 425. DOI: https://doi.org/10.17660/ActaHortic.1997.425.25
DeWald, M., Moore, G., Sherman, W., & Evans, M. (1988). Production of pineapple plants in vitro. Plant cell reports, 7(7), 535-537. DOI: https://doi.org/10.1007/BF00272750
Escalona, M., Lorenzo, J., González, B., Daquinta, M., González, J., Desjardins, Y., & Borroto, C. (1999). Pineapple (Ananas comosus L. Merr) micropropagation in temporary immersion systems. Plant cell reports, 18(9), 743-748. DOI: https://doi.org/10.1007/s002990050653
Fitchet, M. (1990). Clonal propagation of Queen and Smooth Cayenne pineapples. International Symposium on the Culture of Subtropical and Tropical Fruits and Crops 275. DOI: https://doi.org/10.17660/ActaHortic.1990.275.31
Gangopadhyay, G., Bandyopadhyay, T., Poddar, R., Gangopadhyay, S. B., & Mukherjee, K. K. (2005). Encapsulation of pineapple micro shoots in alginate beads for temporary storage. Current Science, 972-977.
Hamad, A. M. (2016). Effect of peatmoss and sand mixing ratio on the acclimatization of Smooth cayenne pineapple (Ananas comosus (L) Merr.). J. of Science and Humanities, 22: 1-6. DOI: https://doi.org/10.37376/1571-000-022-001
Hamad, A. M. (2017a). Effect of incubation periods, medium volumes and explants density on the in vitro shoot formation and growth and cost of multiplication of Moris pineapple. El Mukhtar J. of Sciences, 33 (1): 10 -16.
Hamad, A. M. (2017b). Effect of explants density, medium volumes and subcultures on the in vitro shoot formation and cost of multiplication of Moris pineapple (Ananas comosus L. Merr). Libyan J of Basic Sciences 7 (1): 78- 90. DOI: https://doi.org/10.37376/1570-000-015-010
Hamad, A. M. (2017c). Biweekly changes in the in vitro shoot formation and growth pattern of pineapple (Ananas comosus L Merr) cv Smooth cayenne over 105 days of incubation on different hormone treatments. Globial Libyan J. 18: 1- 17 DOI: https://doi.org/10.37376/1570-000-018-002
Hamad, A. M., and Taha R. M. (2008). Effect of sequential subcultures on in vitro proliferation capacity and shoot formations pattern of pineapple (Ananas comosus L. Merr.) over different incubation periods. Scientia Horticulturae 117(4):329-334.
Hamad, A. M., Taha R. M., and Mohajer S. (2013). In vitro induction and proliferation of adventitious roots in pineapple (Ananas comosus L.) cultivars of smooth cayenne and morris. Australian Journal of Crop Science 7(7):1038-1045.
Hamad, A. H. M., Taha, R. M., & Mohajer, S. (2013). In vitro induction and proliferation of adventitious roots in pineapple (Ananas comosus L.) cultivars of smooth cayenne and morris. Australian Journal of Crop Science, 7(7), 1038-1045.
Hamad, A. M., & Taha, R. M. (2008). Effect of sequential subcultures on in vitro proliferation capacity and shoot formations pattern of pineapple (Ananas comosus L. Merr.) over different incubation periods. Scientia Horticulturae, 117(4), 329-334. DOI: https://doi.org/10.1016/j.scienta.2008.05.009
Khan, S., Nasib A., and Saeed B. A. (2004). Employment of in vitro technology for large scale multiplication of pineapples (Ananas comosos). Pakistan Journal of Botany 36(3):611-616.
Khatun, M., Khanam D., Hoque M., and Quasem A. (1997). Clonal propagation of pineapple through tissue culture. Plant Tissue Cult 7(2):143-148.
Ko, H., Campbell P., Jobin-Décor M., Eccleston K., Graham M., and Smith M. (2006). The introduction of transgenes to control blackheart in pineapple (Ananas comosus L.) cv. Smooth Cayenne by microprojectile bombardment. Euphytica 150(3):387.
Kofi, O., and Adachi T. (1993). Effect of cytokinins on the proliferation of multiple shoots of pineapple in vitro. SABRAO Journal 25(1):59-69.
Mathews, V. H., and Rangan T. (1979). Multiple plantlets in lateral bud and leaf explant in vitro cultures of pineapple. Scientia Horticulturae 11(4):319-328.
Mathews, V. H., and Rangan T. (1981). Growth and regeneration of plantlets in callus cultures of pineapple. Scientia Horticulturae 14(3):227-234.
Mengesha, A., Ayenew B., and Tadesse T. (2013). Energy sources affect in vitro propagation and subsequent acclimatization of Ananas comosus, var. smooth cayenne plants. The Journal of Microbiology, Biotechnology and Food Sciences 2(6):2372
Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassay with tobacco tissue. Physiol. Plan/ 1962, 15: 473- 497 DOI: https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Ramirez-Malagon, R., Borodanenko A., Barrera-Guerra J. L., and Ochoa-Alejo N. (2001). Shoot number and shoot size as affected by growth regulators in in vitro cultures of Spathiphyllum floribundum L. Scientia Horticulturae 89(3):227-236.
Singh, D., and Manual A. (2000). Assessment of pineapple plants developed from micropropagation instead of conventional suckering. Tropical Science 40(4):169-173.
Soneji, J. R., Rao P., and Mhatre M. (2002a). In vitro Regeneration from Leaf Explants of Pineapple (Ananas comosus L, Merr). Journal of plant biochemistry and biotechnology 11(2):117-119.
Soneji, J. R., Rao P., and Mhatre M. (2002b). Somaclonal variation in micropropagated dormant axillary buds of pineapple (Ananas comosus L., Merr.). The Journal of Horticultural Science and Biotechnology 77(1):28-32.
Souza, F. V. D., Da Silva M. D. J., Souza A. D. S., and Costa M. A. P. D. C. (2016). Growth regulators and physical state of culture media in the micropropagation of ornamental pineapple hybrids. Plant Cell Culture & Micropropagation 8(1-2):10-17.
Sripaoraya, S., Marchant R., Brian Power J., and Davey M. R. (2003). Plant regeneration by somatic embryogenesis and organogenesis in commercial pineapple (Ananas comosus L.). In Vitro Cellular and Developmental Biology-Plant 39(5):450-454.
Sunitibala Devi, Y., Mujib A., and Kundu S. (1997). Efficient regenerative potential from long term culture of pineapple. Phytomorphology 47(3):255-259.
Teng, W.-L. (1997). An alternative propagation method ofAnanas through nodule culture. Plant cell reports 16(7):454-457.
Khan, S., Nasib, A., & Saeed, B. A. (2004). Employment of in vitro technology for large scale multiplication of pineapples (Ananas comosos). Pakistan Journal of Botany, 36(3), 611-616.
Khatun, M., Khanam, D., Hoque, M., & Quasem, A. (1997). Clonal propagation of pineapple through tissue culture. Plant Tissue Cult, 7(2), 143-148.
Ko, H., Campbell, P., Jobin-Décor, M., Eccleston, K., Graham, M., & Smith, M. (2006). The introduction of transgenes to control blackheart in pineapple (Ananas comosus L.) cv. Smooth Cayenne by microprojectile bombardment. Euphytica, 150(3), 387. DOI: https://doi.org/10.1007/s10681-006-9124-5
Kofi, O., & Adachi, T. (1993). Effect of cytokinins on the proliferation of multiple shoots of pineapple in vitro. SABRAO Journal, 25(1), 59-69.
Mathews, V. H., & Rangan, T. (1979). Multiple plantlets in lateral bud and leaf explant in vitro cultures of pineapple. Scientia Horticulturae, 11(4), 319-328. DOI: https://doi.org/10.1016/0304-4238(79)90016-5
Mathews, V. H., & Rangan, T. (1981). Growth and regeneration of plantlets in callus cultures of pineapple. Scientia Horticulturae, 14(3), 227-234. DOI: https://doi.org/10.1016/0304-4238(81)90017-0
Mengesha, A., Ayenew, B., & Tadesse, T. (2013). Energy sources affect in vitro propagation and subsequent acclimatization of Ananas comosus, var. smooth cayenne plants. The Journal of Microbiology, Biotechnology and Food Sciences, 2(6), 2372.
Ramirez-Malagon, R., Borodanenko, A., Barrera-Guerra, J. L., & Ochoa-Alejo, N. (2001). Shoot number and shoot size as affected by growth regulators in in vitro cultures of Spathiphyllum floribundum L. Scientia Horticulturae, 89(3), 227-236. DOI: https://doi.org/10.1016/S0304-4238(00)00236-3
Singh, D., & Manual, A. (2000). Assessment of pineapple plants developed from micropropagation instead of conventional suckering. Tropical Science, 40(4), 169-173.
Soneji, J. R., Rao, P., & Mhatre, M. (2002a). In vitro Regeneration from Leaf Explants of Pineapple (Ananas comosus L, Merr). Journal of plant biochemistry and biotechnology, 11(2), 117-119. DOI: https://doi.org/10.1007/BF03263147
Soneji, J. R., Rao, P., & Mhatre, M. (2002b). Somaclonal variation in micropropagated dormant axillary buds of pineapple (Ananas comosus L., Merr.). The Journal of Horticultural Science and Biotechnology, 77(1), 28-32. DOI: https://doi.org/10.1080/14620316.2002.11511452
Souza, F. V. D., Da Silva, M. D. J., Souza, A. D. S., & Costa, M. A. P. D. C. (2016). Growth regulators and physical state of culture media in the micropropagation of ornamental pineapple hybrids. Plant Cell Culture & Micropropagation, 8(1-2), 10-17.
Sripaoraya, S., Marchant, R., Brian Power, J., & Davey, M. R. (2003). Plant regeneration by somatic embryogenesis and organogenesis in commercial pineapple (Ananas comosus L.). In Vitro Cellular and Developmental Biology-Plant, 39(5), 450-454. DOI: https://doi.org/10.1079/IVP2003445
Sunitibala Devi, Y., Mujib, A., & Kundu, S. (1997). Efficient regenerative potential from long term culture of pineapple. Phytomorphology, 47(3), 255-259.
Teng, W.-L. (1997). An alternative propagation method ofAnanas through nodule culture. Plant cell reports, 16(7), 454-457. DOI: https://doi.org/10.1007/BF01092765
Downloads
Published
How to Cite
License
Copyright (c) 2021 Abdelhamid M Hamad
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Copyright of the articles Published by Almukhtar Journal of Science (MJSc) is retained by the author(s), who grant MJSc a license to publish the article. Authors also grant any third party the right to use the article freely as long as its integrity is maintained and its original authors and cite MJSc as original publisher. Also they accept the article remains published by MJSc website (except in occasion of a retraction of the article).
