Determination of Nicotine in Libyan Smokers' Urine Compared with that of Nonsmokers using Reversed Phase – High Performance Liquid Chromatog-raphy (RP-HPLC)

Galal  Elmanfe; Suad K.  Omar; Noreldin S.Y.  Abdolla; Amna M.  Hassan

doi:10.54172/mjsc.v34i3.323

Authors

Galal Elmanfe Chemistry Department, Faculty of Science, Omar Al-Mukhtar University, El-Bieda, Libya
Suad K. Omar Chemistry Department, Faculty of Science, Omar Al-Mukhtar University, El-Bieda, Libya
Noreldin S.Y. Abdolla Chemistry Department, Faculty of Science, Tobrouk University, Tobrouk, Libya
Amna M. Hassan Chemistry Department, Faculty of Science, Omar Al-Mukhtar University, El-Bieda, Libya

DOI:

https://doi.org/10.54172/mjsc.v34i3.323

Keywords:

Cigarettes, Smoking, Nicotine, Urine, Extraction, RP-HPLC

Abstract

The aim of the present study was to evaluate the levels of nicotine in twenty urine samples taken from ten smokers and ten non-smokers in Libya. Each volunteer was required to complete a questionnaire before providing the urine sample. The evaluation of the nicotine concentrations was carried out by means of a simple, rapid, cost effective but reliable, one-step extraction technique-reversed-phase high-performance liquid chromatography which was developed and validated for this purpose. The criteria and factors taken into consideration for this evaluation and validation include the linearity, precision, accuracy, limit of detection, and limit of quantitation. The urine samples from the smokers presented nicotine concentrations in the range of 0.037-1.979 µg/ml, with an average of 0.663 µg/ml. The range of the nicotine concentrations in non-smokers, on the other hand, was from 0.017-1.331 g/ml, where 0.273 µg/ml is the average value. Statistical analyses show that the nicotine concentrations were very significant in the smoker samples in contrast with the nonsmoker samples

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References

Allen, J. A. V., D. Haviland, M. L. Healton, C. Davis, K. C. Farrelly, M. C. Husten, & C. G. Pechacek, T. (2003). Tobacco use among middle and high school students, United States, 2002. Morbidity and Mortality Weekly Report, 52(1096), 1096.

Ceppa, F., El Jahiri, Y., Mayaudon, H., Dupuy, O., & Burnat, P. (2000). High performance liquid chromatographic determination of cotinine in urine in isocratic mode. Journal of Chromatography B: Biomedical Sciences and Applications, 746(2), 115-122. DOI: https://doi.org/10.1016/S0378-4347(00)00306-6

Davoli, E., Stramare, L., Fanelli, R., Diomede, L., & Salmona, M. (1998). Rapid solid-phase extraction method for automated gas chromatographic–mass spectrometric determination of nicotine in plasma. Journal of Chromatography B: Biomedical Sciences and Applications, 707(1), 312-316. DOI: https://doi.org/10.1016/S0378-4347(97)00579-3

Elmanfe, G., & Abdulla, A. (2014). Spectrophotometric Determination of Nicotine in Some Libyan Smokers’ Blood and Compared with Nonsmokers. Paper presented at the 1st Conference on Chemical Sciences and its Applications, 21-23 April 2014, Elbaida-Libya

Giovino, G. A. (1999). Epidemiology of tobacco use among US adolescents. Nicotine and Tobacco Research 1, (Suppl 1):, S31-40. DOI: https://doi.org/10.1080/14622299050011571

Gupta, R., Prakash, H., Gupta, V. P., & Gupta, K. D. (1997). Prevalence and determinants of coronary heart disease in a rural population of India. Journal of Clinical Epidemiology, 50(2), 203-209. DOI: https://doi.org/10.1016/S0895-4356(96)00281-8

Hamm, J. G. (1982). The Handbook of Pest Control (1st ed.). Frederick Fell Publishers Inc.: New York.

Jha, P., Jacob, B., Gajalakshmi, V., Gupta, P., Dhingra, N., Kumar, R., . . . Richard, P. (2008). A Nationally Representative Case Control Study of Smoking and Death in India. New England Journal of Medicine, 358(11), 1137-1147. DOI: https://doi.org/10.1056/NEJMsa0707719

Jones, F. E. (1994). Toxic Organic Vapors in the Workplace. Lewis Publishers: London, CRC Press Inc.: New York. 224.

Massadeh, A. M., Gharaibeh, A. A., & Omari, K. W. (2009). A Single-Step Extraction Method for the Determination of Nicotine and Cotinine in Jordanian Smokers' Blood and Urine Samples by RP-HPLC and GC-MS. Journal of Chromatographic Science, 47(2), 170-177. DOI: https://doi.org/10.1093/chromsci/47.2.170

Orlando, M., Tucker Joan S, Ellickson Phyllis L., & J., K. D. (2004). Developmental Trajectories of Cigarette Smoking and Their Correlates From Early Adolescence to Young Adulthood. Journal of Consulting and Clinical Psychology, 72(3), 400-410. DOI: https://doi.org/10.1037/0022-006X.72.3.400

Padmavati, S. (2002). Prevention of heart disease in India in the 21st century: need for a concerted effort. Indian Heart J, 54(1), 99-102.

Pasupathi, P., Bakthavathsalam, G., Rao, Y. Y., & Farook, J. (2009). Cigarette smoking effect of metabolic health risk: A review. Diabetes & Metabolic Syndrome: Clinical Research & Reviews, 3(2), 120-127. DOI: https://doi.org/10.1016/j.dsx.2009.02.006

Pierce, P., & Gilpin, E. (1996). How long will today’s new adolescent smoker be addicted to cigarettes? American journal of public health, 86, 253 - 256. DOI: https://doi.org/10.2105/AJPH.86.2.253

Rani, M., Bonu, S., Jha, P., Nguyen, S. N., & Jamjoum, L. (2003). Tobacco use in India: prevalence and predictors of smoking and chewing in a national cross sectional household survey. Tobacco Control, 12(4), 1-8. DOI: https://doi.org/10.1136/tc.12.4.e4

Rodricks, J. W. (1992). Calculated Risks (1st ed.). Cambridge University Press. pp. 251- 256.

Shin, H. S., Kim, J. G., Shin, Y. J., & Jee, S. H. (2002). Sensitive and simple method for the determination of nicotine and cotinine in human urine, plasma and saliva by gas chromatography mass spectrometry. Journal of Chromatography B, 769(1), 177-183. DOI: https://doi.org/10.1016/S1570-0232(02)00007-7

Stevens, K. R., A. Marie Barron, Carol A. Ledbetter, Katie M. Foarde, & Menard., S. W. (2001). Legislation, Policy, and Tobacco Use Among Youth: Implications for Health Care Providers. Journal of School Health, 71(3), 89-95. DOI: https://doi.org/10.1111/j.1746-1561.2001.tb07298.x

Tollison, D. R. (1988). Clearing the air: perspectives on environmental tobacco smoke: Lexington, Mass.: Lexington Books, MA, U.S.A.

U.S. Department of Health and Human Services. (2000). Healthy People 2010: Understanding and Improving Health (2nd ed.): Washington, DC: U.S. Government Printing Office.

Watson, R. R., & Witten, M. (2001). Environmental Tobacco Smoke. Boca Raton London New York Washington, D.C.: CRC Press.

Wills, T. A., & Cleary, S. D. (1997). The validity of self-reports of smoking: analyses by race/ethnicity in a school sample of urban adolescents. American journal of public health, 87(1), 56-61. DOI: https://doi.org/10.2105/AJPH.87.1.56

Wu, C., Siems, W. F., Hill, J. H. H., & Hannan, R. M. (1998). Analytical determination of nicotine in tobacco by supercritical fluid chromatography ion mobility detection. Journal of Chromatography A, 811(1), 157-161. DOI: https://doi.org/10.1016/S0021-9673(98)00223-4