دراسات فسيولوجية ونسيجية على تأثير  الهيدروكورتيزون على الكلى  في الأرانب البيضاء النيوزيلاندية

Nura I. Al-Zail; Abdusalam M. Aboalhaj; Zainab A. Shuaib

doi:10.54172/mjsc.v32i1.126

Authors

Nura I. Al-Zail Zoology Department, Faculty of Science, Omar Al-Mukhtar University, Libya.
Abdusalam M. Aboalhaj Zoology Department, Faculty of Science, Omar Al-Mukhtar University, Libya.
Zainab A. Shuaib Zoology Department, Faculty of Science, Omar Al-Mukhtar University, Libya.

DOI:

https://doi.org/10.54172/mjsc.v32i1.126

Keywords:

hydrocortisone, kidney, physiological, histological, rabbits

Abstract

This study aimed to test the effect of hydrocortisone sodium succinate drug on blood serum components and kidneys tissues in white rabbits.The experiment included 30 male rabbits weighing between 1500-2500g. They were divided into 4 control and treated groups, for different periods of time, depending on the duration of injection. Treatment of the rabbits with hydrocortisone dose of 10 mg/kg daily did not lead to change in the weights of rabbits treated for a week. Non significant increase occurred in the weights of rabbits treated for two weeks and treated gradually, but in sudden treated group a non significant decrease in rabbits weights was recorded in comparison with their weights before injection .A significant increase in the concentration of urea, creatinine, total serum protein, albumin, sodium and potassium ions after treatment for a week, but a significant reduction was noted in the concentration of urea and no significant difference was noted in the concentration of creatinine, sodium and potassium ions after treatment for two weeks. While there were a significant increase in the total serum protein and albumin after two weeks of treatment. Noting that, in the suddeny treated group, the biochemical parameters did not change compared to that present in the two weeks treated rabbits. While, in the gradually treated group, most of the biochemical parameters returned to their normal values .Histological examination of the renal cortex showed acidophilic material accumulated in the lumina of some distal convoluted tubules in the group treated for two weeks. The renal medulla also showed the presence of the same material inside the collecting tubules in the both treated groups. With the increase of the duration of treatment; the intracytoplasmic vacuoles appeared in many of the cells lining the collecting tubules. The same histopathological changes were observed in the kidneys of rabbits that their treatment was suddenly stopped, as well as the group that their treatment was gradually stopped, but these changes became low in the last group.

Downloads

Download data is not yet available.

References

زايد. عبد الله عبد الرحمن محمد و توني محمد خلف. (1998). علم وظائف الأعضاء الغدد الصماء والهرمونات، الطبعة الأولى، منشورات جامعة عمر المختار، ليبيا،51-63.

خليل، محمد مدحت حسين. (1997). علم الغدد الصماء، مكتبة المدينة- العين -الإمارات العربية المتحدة، جامعة الازهر،177-233.

خليل، محمد مدحت حسين. (2005). فسيولوجيا الحيوان، الطبعة الثانية، دار الكتاب الجامعي، العين، الإمارات العربية المتحدة،429-463.

خليل، محمد مدحت حسين. (2012). فسيولوجيا الإنسان، الطبعة الأولى، دار الكتاب الجامعي، العين، الإمارات العربية المتحدة، 379-446.

شيفيل نورمان. (1982). أمراض الخلية . ترجمة .غياث صالح محمود .(1997). الطبعة الأولى . مطبوعات جامعة الموصل . العراق .

محيي الدين. خير الدين؛ وليد حميد يوسف و سعد حسين توحلة. (1990). فسلجة الغدد الصم والتكاثر في الثدييات والطيور، دار الحكمة للطباعة والنشر، الموصل، العراق.

Abraham, G., Gottschalk, J., and Ungemach, F. R. (2005). Evidence for ototopical glucocorticoid-induced decrease in hypothalamic-pituitary-adrenal axis response and liver function. Endocrinology 146(7):3163-3171. DOI: https://doi.org/10.1210/en.2005-0080

Ali, R., Amlal, H., Burnham, C. E., and Soleimani, M. (2000). Glucocorticoids enhance the expression of the basolateral Na+: HCO 3-cotransporter in renal proximal tubules. Kidney international 57(3):1063-1071. DOI: https://doi.org/10.1046/j.1523-1755.2000.00933.x

Baas, J., Schaeffer, F., and Joles, J. (1984). The influence of cortisol excess on kidney function in the dog. Veterinary Quarterly 6(1):17-21. DOI: https://doi.org/10.1080/01652176.1984.9693901

Bancroft, J. D., and Gamble, M. (2008). Theory and practice of histological techniques. Elsevier Health Sciences.

Boykin, J., Detorrenté, A., Erickson, A., Robertson, G., and Schrier, R. W. (1978). Role of plasma vasopressin in impaired water excretion of glucocorticoid deficiency. Journal of Clinical Investigation 62(4):738. DOI: https://doi.org/10.1172/JCI109184

Carlton, W., McGavin, M. D., Thomson, R., and William, W. C. (1995). Thomson's special veterinary pathology/Special veterinary pathology.

Cassano, C., Fabbrini, A., Andres, G., Cinotti, G., DeMartino, C., and Minio, F. (1964). Functional, light and electron microscopic studies of the kidney in myxoedema. Eur Rev Endocrinol 1(1-10.

DiScala, V., Salomon, M., Grishman, E., and Churg, J. (1967). Renal structure in myxedema. Archives of pathology 84(5):474-485.

Elshennawy, W. W., and Elwafa, H. R. A. (2011). Histological and ultrastructural changes in mammalian testis under the effect of hydrocortisone. Journal of American Science 7(9):38-48.

Gevorgyan, E., Yavroyan, Z. V., Galstyan, A., and Demirkhanyan, L. (2008). Content Of Some Phospholipid Fractions In Rat Liver Nuclei After The In Vivo Action Of Hydrocortisone AND INSULIN. Electronic Journal of Natural Sciences 10(1).

Gloor, B., Uhl, W., Tcholakov, O., Roggo, A., Mũller, C., Worni, M., and Bũchler, M. (2001). Hydrocortisone treatment of early SIRS in acute experimental pancreatitis. Digestive diseases and sciences 46(10):2154-2161. DOI: https://doi.org/10.1023/A:1011902729392

Jubb, K. V. F. (1985). PATHOLOGY OF DOMESTIC ANIMALS 3E. Academic press.

Katz, A. I., Emmanouel, D. S., and Lindheimer, M. D. (1975). Thyroid hormone and the kidney. Nephron 15(3-5):223-249. DOI: https://doi.org/10.1159/000180514

Kawai, K., Tamaki, A., and Hirohata, K. (1985). Steroid-induced accumulation of lipid in the osteocytes of the rabbit femoral head. A histochemical and electron microscopic study. JBJS 67(5):755-763. DOI: https://doi.org/10.2106/00004623-198567050-00010

Levenson, D. J., Simmons, C. E., and Brenner, B. M. (1982). Arachidonic acid metabolism, prostaglandins and the kidney. The American journal of medicine 72(2):354-374. DOI: https://doi.org/10.1016/0002-9343(82)90826-9

Lipworth, B. J. (1999). Systemic adverse effects of inhaled corticosteroid therapy: a systematic review and meta-analysis. Archives of Internal Medicine 159(9):941-955. DOI: https://doi.org/10.1001/archinte.159.9.941

Mandal, S. K. (2007). Effect of glucocorticoid on protein and creatine content of inactivated muscle of rats.

Mangos, G. J., Whitworth, J. A., Williamson, P. M., and Kelly, J. J. (2003). Glucocorticoids and the kidney. Nephrology 8(6):267-273. DOI: https://doi.org/10.1111/j.1440-1797.2003.00215.x

Müller, A. F., and O'Connor, C. M. (1958). An international symposium on aldosterone. Little, Brown.

Parker, A., Hamlin, G., Coleman, C., and Fitzpatrick, L. (2003). Dehydration in stressed ruminants may be the result of acortisol-induced diuresis. Journal of Animal Science 81(2):512-519. DOI: https://doi.org/10.2527/2003.812512x

Piffer, R., and Pereira, O. (2004). Reproductive aspects in female rats exposed prenatally to hydrocortisone. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 139(1):11-16. DOI: https://doi.org/10.1016/j.cca.2004.08.017

Rodrigues-Mascarenhas, S., Dos Santos, N. F., and Rumjanek, V. M. (2006). Synergistic effect between ouabain and glucocorticoids for the induction of thymic atrophy. Bioscience Reports 26(2):159-169. DOI: https://doi.org/10.1007/s10540-006-9012-1

Smets, P., Meyer, E., Maddens, B., and Daminet, S. (2010). Cushing’s syndrome, glucocorticoids and the kidney. General and Comparative Endocrinology 169(1):1-10. DOI: https://doi.org/10.1016/j.ygcen.2010.07.004

Tai, Y.-H., Decker, R. A., Marnane, W. G., Charney, A. N., and Donowitz, M. (1981). Effects of methylprednisolone on electrolyte transport by in vitro rat ileum. American Journal of Physiology-Gastrointestinal and Liver Physiology 240(5):G365-G370. DOI: https://doi.org/10.1152/ajpgi.1981.240.5.G365

Tata, D. A., and Anderson, B. J. (2010). The effects of chronic glucocorticoid exposure on dendritic length, synapse numbers and glial volume in animal models: implications for hippocampal volume reductions in depression. Physiology & behavior 99(2):186-193. DOI: https://doi.org/10.1016/j.physbeh.2009.09.008

Walker, S. E., and Schnitzer, B. (1980). Resistance to therapy in mature Palmerston North mice treated with cyclophosphamide or hydrocortisone sodium succinate. Arthritis & Rheumatology 23(5):539-544. DOI: https://doi.org/10.1002/art.1780230504

Yarushkina, N. (2008). The role of hypothalamo-hypophyseal-adrenocortical system hormones in controlling pain sensitivity. Neur. and Behavioral Physiology 38(8):759-766. DOI: https://doi.org/10.1007/s11055-008-9044-z