Physiochemical and histological study on the effect of the hibernation on the liver of Uromastyx acanthinura (Bell, 1825).


  • Yousef K.A Abdel-Hafid Department of Zoology, Faculty of Science, Omar Al-Mukhtar University, Libya



Hibernation, , histology of liver, DNA fragmentation, caspase 3 – caspase 7


This study described the changes in the liver of Uromastyx acanthinura (Bell, 1825) males and females during hibernation and activity seasons. The results revealed that, hibernation causes increase fatty liver and pigment cells with abundant damage, comparing with nearly normal structure and less fatty liver after the hibernation with almost normal pattern. Genomic DNA showed apparent separation during hibernation. Also, caspase3 and caspase7 activity  reached a high level in the liver tissue during hibernation comparing with activity season.


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Abdel-Raheem, K., Okasha, S., El-Deib, S., Shaalan, S. and El Daly, E. (1989a) Hibernation in reptiles: 5- Changes in the nucleic acids metabolism in tissues of Eryx colubrinus and Eumeces Schneideri. Bull. Fac. Sci., Cairo University, 57, 65-80.

Abdel-Raheem, K., Okasha, Shaalan, S. and El-Daly, E. (1989c) Hibernation in reptiles: 4-Changes in amino acids and protein metabolism. Bull. Fac. Sci., Cairo University, 57, 81-99.

Angilletta, M.J., Niewiarowski, P.H. and Navas, C.A. (2002) The evolution of thermal physiology in ectotherms. J. Thermal Biol., 27, 249–268. DOI:

Arends, M.J., Morris, R.J. and Wyillie, A.H. (1990) Apoptosis: The role of the endonuclease. Am. J. Pathol., 136, 593–608.

Arnold, S.J. and Peterson, C.R. (1989) A test for temperature effects on the ontogeny of shape in the garter snake, Thamnophis sirtalis. Physiol. Zool., 62, 1316–1333. DOI:

Avery, R.A. (1994) Growth in reptiles. Gerontology, 40, 193–199. DOI:

Beaupre, S.J. (1995) Effects of geographically variable thermal environment on bioenergetics of mottledrattle snakes, Crotalus lepidus. Ecology, 76, 1655–1665. DOI:

Beaupre, S.J. (1996) Fieldmetabolic rate, water flux, and energy budgets of mottled rattle snakes, Crotalus lepidus, from two populations. Copeia, 1996, 319–329. DOI:

Beaupre, S.J., Dunham, A.E. and Overall, K.L. (1993) Metabolism of a desert lizard: the effects of mass, sex, population of origin, temperature, time of day, and feeding on oxygen consumption of Sceloporus merriami. Physiol. Zool., 66, 128–147. DOI:

Bennett, A.F., and Dawson, W.R. (1976) Metabolism. In Gans, C., Pough, F.H. (Eds.). Biology of the Reptilia, Academic Press, NY, 5, 127–223.

Bortner, C.D., Oldenburg, N.B and Cidlowski, J.A. (1995). The role of DNA fragmentation in apoptosis. Trends Cell Biol., 5: 21–26. DOI:

Castonguay, M., Rollet, C., Frechet, A., Gagnon, P., Gilbert, D. and Brethes, J.C. (1999) Distribution changes of Atlantic cod (Gadus morhua L.) in the northern Gulf of St Lawrence in relation to an oceanic cooling. ICES J. Marine Sci., 56, 333–344. DOI:

Cicero, R., Mallardi A., Maida, I., Gallone, A., and Pintucci, G. (1989) Melanogenesis in the pigment cells of Rana esculenta L. liver: evidence for tyrosinase-like activity in the melanosome protein fraction. Pigment Cell Research, 2, 100-108. DOI:

Congdon, J.D., Dunham, A.E., and Tinkle, D.W. (1982) Energy budgets and life histories of reptiles. In: Gans, C., Pough, F.H. (Eds.). Biology of the Reptilia, Academic Press, NY, 13, 155–199.

Crawford, K.M. (1991) The winter environment of painted turtles, Chrysemys picta: temperature, dissolved oxygen, and potential cues for emergence. Can. J. Zool., 69, 2493-2498. DOI:

Dunham, A.E, Grant, B.W. and Overall, K.L. (1989) Interfaces between biophysical and physiological ecology and the population ecology of terrestrial vertebrate ectotherms. Physiol. Zool., 62, 335–355. DOI:

Frerichs, K.U., Dienel, G.A., Cruz, N.F., Sokoloff, L. and. Hallenbeck, J.M. (1995) Rates of glucose utilization in brain of active and hibernating ground squirrels. Am. J. Physiol., 268, 445–453. DOI:

Frerichs, K.U., Kennedy, C., Sokoloff, L. and Hallenbeck, J. M. (1994) Local cerebral blood flow during hibernation; a model of natural tolerance to cerebral ischemia, J. Cereb. Blood Flow Metab., 14, 193– 205. DOI:

Gatten Jr.R.E. (1974) Effects of temperature and activity on aerobic and anaerobic metabolism and heart rate in the turtles Pseudemys scripta and Terrapene ornata. Comp. Biochem. Physiol., 48A, 619–648. DOI:

Grobman, A. B. (1990) The effect of soil temperatures on emergence from hibernation of Terrapene carolina and T. ornata. Am. Midl. Nat., 124, 366-371. DOI:

Karasov, W.H. and Anderson, R.A., (1998) Correlates of average daily metabolism of field-active zebra-tailed lizards (Callisaurus draconoides). Physiol. Zool., 71, 93–105. DOI:

Kruman, I.I., Kolaeva, S.G. Iljasova, E.N., Zubrikhina, G.N., Khachko V.N. and Petrova, A.S. (1986) Seasonal variations of DNA synthesis in intestinal Epithelial cells of hibernating animals I. DNA synthesis in intestinal epithelial cells of ground squirrel Citellus undulates during deep hibernation. Comp. Biochem. Physiol., 83, 173-177. DOI:

Lillywhite, H.B. (1987) Temperature, energetics, and physiological ecology. In: Seigel, R.A., Collins, J.T., Novak, S.S. (Eds.). Snakes: Ecology and Evolutionary Biology. Macmillan Publishing Co., NY, 422–477. DOI:

Litzgus, J.D. and Brooks, R.J. (1998a) Growth in a cold environment: body size and sexual maturity in a northern population of spottedturtles, Clemmys guttata. Can. J. Zool., 76, 773–782. DOI:

Litzgus, J.D. and Brooks, R.J. (1998b) Reproduction in a northern population of Clemmys guttata. J. Herpetol., 32, 252–259. DOI:

Macartney, J.M., Larsen, K.W. and Gregory, P.T. (1989) Body temperatures and movements of hibernating snakes (Crotalus and Thamnophis) and thermal gradients of natural hibernacula. Can. J. Zool., 67, 108-114. DOI:

McNab, B.K. (2002) The Physiological Ecology of Vertebrates: A View from Energetics. Comstock Publishing Associates, Cornell University Press, Ithaca, NY.

Rock, J. and Cree, A. (2003) Intraspecific variation in the effect of temperature on pregnancy in the viviparous gecko Hoplodactylus maculatus. Herpetologica, 59, 8–22. DOI:[0008:IVITEO]2.0.CO;2

Scalla, M., Geremia, E., Corsaro, C., Santoro, C., Baratta, D. and Sichel, G. (1990) Lipid peroxidation in pigmented and unpigmented liver tissue: protective role of melanin. Pigment Cell Research, 3, 115-119. DOI:

Schwarzkopf, L. and Shine, R. (1991) Thermal biology of reproduction in viviparous skinks, Eulamprus tympanum— why do gravid females bask more. Oecologia, 88, 562–569. DOI:

Secor, S.M. and Nagy, K.A. (1994) Bioenergetic correlates of foraging mode for the snakes Crotalus cerastes and Masticophis flagellum. Ecology, 5, 1600–1614. DOI:

Squire, T.L. and Andrews, M.T. (2003) Pancreatic triacylglycerol lipase in a hibernating mammal. 1. Novel genomic organization. Physiol. Genomics., 16, 119-130. DOI:

Waldschmidt, S.R., Jones, S.M. and Porter, W.P. (1987) Reptilia. In: Panadian, T.J., Vernberg, F.J. (Eds.). Animal Energetics, Academic Press, NY, 2, 553–619. DOI:

Webb, J.K. and Shine, R. (1998) Using thermal ecology to predict retreat-site selection by an endangered snake species. Biol. Conserv., 86, 233–242. DOI:

Whitaker, P.B. and Shine, R. (2002) Thermal biology andactivity patterns of the eastern brown snake (Pseudonaja textilis): a radiotelemetric study. Herpetologica, 58, 436–452. DOI:[0436:TBAAPO]2.0.CO;2

Zuasti, A.; Ferrer, C., Aroca, P. and Solano, F. (1990) Distribution of extracutaneous melanin pigment in Sparus auratus, Mugil cephalus, and Dicentrarchus labrax (Pisces, Teleostei). Pigment Cell Research, 3, 126-131. DOI:




How to Cite

Abdel-Hafid, Y. K. (2013). Physiochemical and histological study on the effect of the hibernation on the liver of Uromastyx acanthinura (Bell, 1825). Al-Mukhtar Journal of Sciences, 28(2), 1–11.



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