An investigation of allelopathic effects of Artemisia herba-alba Asso, aqueous extract on common bean (Phaseolus vulgaris L.)


  • Muoftah A Bataw Faculty of Natural Resources & Environmental Sciences, Omar Al-Mukhtar University
  • Jackie M. Hughes School of Environment and Life Sciences, Biomedical Institute of the University of Salford, UK



Allelopathy, Artemisia herba-alba, Seedling Growth, Secondary metabolites


The objective of this study was to investigate the impact of allelopathic potentials of aqueous extract of Artemisia herba-alba aerial parts on germination and secondary metabolites accumulation in common bean seedlings. Quantitative analysis of aqueous extract of Artemisia showed the phenolic compounds and flavonoids that might be concerned as allelo-chemicals agents. After 8 days, the germinated common bean seedlings were significantly inhibited with the increase in concentration of Artemisia extract. However, the phenolic compounds in common bean seedlings increases when the concentration of Artemisia extract increased. Degradation of storage carbohydrates of common bean seedlings was significantly increased with increasing the concentration of Artemisia extract. It seems that the enhance-ment of total carbohydrates was attributed to the inhibition of amylase activity that reduced the contents of reducing and non-reducing sugars. However, the polysaccharides remained at high level when compared with those of the control. Inhibition of protease activity led to accumulation of free amino acids and protein contents in germinated seedlings of common bean. Although, 4% aqueous extract of Artemisia shoots contains allelopathic chemicals and phytotoxic, this effect led to an increase in unsaturated and saturated fatty acids in germinated common bean seedlings.


Download data is not yet available.


Metrics Loading ...


Abdel-Fattah R.I., Abou–Zeid A.M. and Atalhi A.D. (2011) Allelopathic effects of Artemisia priceps and Launae sonchoids on rhizospheric fungi and Wheat growth. Afr. J. Microbiol. Res., 5, 41-424.

Adrian E., Maria J.A., Jose M.P. and Felix P.G. (2000) Inhibitory effects of Artemisia herba-alba on the germination of Gypsphyte Helianthemum squamatum. Plant Ecol., 148, 71-80.

Afifi W.M., Ahmad, M.I., Zeinab, A. and Abdul-Hamid, M.F. (1986) Effect of Gamma irradiation and GA3 on amylase activity of pea seedlings. Ann. Agri. Sci., Moshtohor, 24, 2047- 2057.

Alam, S.M., Ala, S.A., Ansar, R. and Khan, M.A. (2001) Influence of leaf extract of Bermuda grass (Cynodon dactylon L.) on the germination and seedling growth of wheat. Wheat Information Service, 92, 17-19.

Al-Charchafchi, F.M.R., Redha, F.M.J. and Kamal, W.M. (1987) Dormancy of Artemisia herba alba seeds in relation to endogenous chemical constituents. J. Biol. Sci. Res. Baghdad, Iraq, 2, 1-2.

Alexa, N., Seal, J.E., Terry, H.L. and Lewin, G. (2004) Screening rice varieties for an aquatic weed infesting Australian Riverina rice crops. Australian J. Agric. Rec., 55, 673-679. DOI:

Al-Shamoany, L.S., Al-Khazraji, M. and Twij, H.A. (1994) Hypolycaemic effect of Artemisia herba alba. J. Ethanopharmacol. 43,167-171. DOI:

An, M., Johnson, I.R. and Lovett, I.R. (1993) Mathematical modeling of allelopathy: biological response to allelochemicals and its interpretation. J. Chem.. Ecol., 19, 2379–2388. DOI:

An, M., Haig, T. and Partly, J.E. (2000) Phytotoxicity of Vulpia residues II Separation, identification and quantitation of allelochemicals from Vulpia myuros. J. Chem. Ecol., 26, 1464-1476.

Berhow, M.A. and Voughn, S.F. (1999) Higher plant flavanoids: biosynthesis and chemical ecology: 423– 438. In: Inderjit, K.; Dakshini, M.M. and Foy, C.L. (Eds), Principles and practices in plant Ecology: Allelochemical Interactions.

Blackburn, S. (1986) Amino acids determination (Methods and Techniques). Edward Arnold. Ltd., London, Marcel, Dakker, Inc., New York.

Chaves, N. and Escudero, C. (2006) Effect of allelopathic compounds produced by Cistus ladenifer on germination of 20 Mediterranean taxa. Plant Ecol., 184, 259- 272. DOI:

El-Darier, S.M. (2002) Allelopathic effects of Eucalyptus rostrata on growth; nutrient uptake and metabolite accumulation of Vicia faba L. and Zea mays L. Pak. J. Biol. Sci., 5, 6-11. DOI:

El-Khatib, A.A. and Hegazy, A.K. (1999) Growth and physiological responses of wild oats allelopathic potential of wheat. Acta Agron. Hung., 47, 11–18.

El-Khatib, A.A., Hegazy, A.K. and Galal, H.K. (2004) Allelopathy in the rhizosphere and amended soil of Chenopodium murale L. Weed Biol. Manag., 4, 35-42. DOI:

El-Khawase, S.A. and Shehata, M.M. (2005) The allelopathic potentialities of Acacia nilotica and Eucalyptus rostrata on monocot (Zea mays L.) and dicot (Phaseolus vulgaris L.) plants. Biotech., 4, 23–34. DOI:

Einhelling, F.A. (1996) Interaction involving allelopathy in cropping systems. Agron J. 88, 886–893. DOI:

Ercisli, S., Esitken, A., Turkkal, C. and Orhan, E. (2005) The allelopathic effects of juglone and walnut leaf extract on yield, growth, chemical and PNE composition of strawberry cv. Fern. Plant Soil Environ., 6, 283–287. DOI:

Harborne, J.B. (1984) Phytochemical methods. Chapman and Hall, New York, 2nd edition, 287. DOI:

Hussain, F. and Khan, T.W. (1988) Allelopathic effects of Pakistani weed Cynodon dactylon L. J. Weed Sci. Res., 1, 8–17.

IUPAC, International Union of Pure and Applied Chemistry (1979) Standard methods for the analysis of oils, fats derivatives. Part I, Sections 1 and 2. Method 2301 and 2401 Pergamen Press, Oxford, 6th editions.

Kanitah, B.I. (2011) Ecophysiological and phytochemical changes of some wild plants in Saudi Arabia. Thesis, Bot. Dept., Fac. Sci., King Saud University.

Khan, D., Xuan, T.D. and Chung, I.M. (2007) Rice allelopathy and the possibility for weed management. Ann. Appl. Biol., 151, 325- 339. DOI:

Lixf Wang, J., Xu, W.B. and Wang, K. (2010) Allelopathic effects of Artemisia frigid on three Poaceae plants seed germination and seedling growth. Ying Yong Sheng Tai Xue Bao., 21,1702-1708.

Lowry, O.H., Rosebrough, N.F., Furr, A.L. and Randell R.F. (1951) Protein measurement with folin phenol reagent. J. Biol. Chem., 193,265-275. DOI:

Modallal, N.M. and Al-Charchafchi, F.M. (2006) Allelopathic effect of Artemisia herba alba on germination and seedling growth of Anabasis setifera. Pakistan J. Biol. Sci., 9, 1795-1798. DOI:

Moussavi-Nik, S., Bijeh, Keshavarzi, M.H. and Gharibdosti, A.B. (2011) Effect of aqueous extracts of allelopathic Artemisia annua on germination and early growth of Ispagol (Plantago ovate). Ann. Biol. Res., 2, 687-691.

Nelson, N. (1944) A phytometric adaptation of the Somogyi method for the determination of glucose. J. Biol. Chem., 153, 375 – 380. DOI:

Ong, P.S. and Gaucher, G.M. (1973) Protease production by thermophilic fungi. Canadian J. Microbiol., 19, 129 – 133. DOI:

Oussama, O. (2003) Allelopathy in two durum wheat (Triticum durum L.) varieties. Agric. Ecos. Environ., 96, 161-163. DOI:

Pandey, D.K. and Mishra, N. (2005) Relative phytotoxicity of an allelochemical hydroquinone to coontail (Ceratophyllum demersum L.) and rice (Oryza sativa L. var. Kranti). In: Proceding and selected papers of the Fourth World Congress on Allelopathy, Charles Sturt University, Wagga NSW, Australia.

Rietjens, I.M. and Alink, G.M. (2003) Nutrition and health-toxic substances in food. Ned. Tijdschr Geneeskd., 147, 2365–2370.

Seal, A.N., Haig, T. and Partley, J.E. (2004) Evaluation of putative allelochemicals in rice rot exudates for their role in the suppression of arrowheat rot growth. J. Chem. Ecol., 30, 1663–1678. DOI:

Somogyi, M. (1952) Note on sugar determination. J. Biol. Chem., 19– 25. DOI:

Weir, T.L., Park, S.W. and Vivanco, J.M. (2004) Biochemical and physiological mechanisms mediated by allelochemicals: Current opinion. Plant Biol., 7, 472– 479. DOI:

Zohary, M. (1973) Goebotanical foundation of Middle East. Vol. I and II Gustraw Fischer Swets and Zeitlinger Amesterdam.




How to Cite

Bataw , M. A. ., & Hughes, J. M. . (2013). An investigation of allelopathic effects of Artemisia herba-alba Asso, aqueous extract on common bean (Phaseolus vulgaris L.) . Al-Mukhtar Journal of Sciences, 28(2), 20–30.



Research Articles