Physiological and Histological Studies on the Effect of Hydrocortisone on Rabbit Liver
Keywords:Hydrocortisone, Liver, Liver Enzymes, Rabbits, Glucocorticoids
The aim of This study was to test the effect of The drug hydrocortisone sodium succinate on blood picture, serum components and liver tissues in white rabbits.The experiment included 30 male Rabbits ranged between 1500-2500g. They were divided into 4 control and treated groups, for different periods of time, depending on the duration of injection. After treatment of the rabbits with a hydrocortisone dose of 10 mg/kg daily for one week and two weeks frequent urination was observed. Swelling of The urinary bladder and congestion of liver was also noticed after slaughtering of the animal. An increase in the amount of adipose tissue around the liver and kidneys in the gradually treated group was observed. Treatment of rabbits with the drug for a week did not lead to change in the weights. Non significant increase occurred in the weights of rabbits treated for two weeks and treated gradually, but in sudden treatmed group a non significant decrease in rabbits weights was recorded in comparison with their weights before injection. The treatment for a week and two weeks led to a significant increase in the levels of the following liver enzymes: aspartate amino transferase(AST), alanin amino transferase (ALT) and alkaline phosphatase enzymes(ALP). The values of these enzymes remained high in both suddenly and gradually treated groups similar to that of the two weeks treated group. Results of biochemical tests showed significant increase in the level of glucose in rabbits treated for a week, but the treatment for two weeks did not lead to a significant increase. In the suddeny treated group, The parameters did not change than that present in the two weeks treated rabbits. While, In the gradually treated group, the blood glucose level returned to the normal value. The histological examination of the livers of rabbits treated for a week revealed congestion and dilatation of central veins. Also in some sections there was hemolysis within the central veins. The same group also showed that there were intracytoplasmic vacuoles in some liver cells. In two weeks treated rabbits these pathological changes became more obvious, in addition, dilataion of hepatic sinusoids occurred. In rabbits in which the treatment was stopped suddenly; the central veins remained dilated, but the congestion and hemolysis were decreased. The dilataion of hepatic sinusoids and vacuolation of hepatocytes were also decreased. As for the rabbits that their treatment was gradually stopped most of the central veins returned to their normal size, but remained full of hemolytic erythrocytes. Hepatic sinusoids also returned to their normal size, but the intracytoplasmic vacuoles remained distributed in the liver cells with a reductioin in their quantity and size. Histochemical examination showed decreased reactivity of liver cells with periodic acid Schiff (PAS) stain in both groups. There was also a decrease in the distribution of glycogen granules in the hepatocytes of the treated rabbits; were most of the cell parts appeared devoid of these granules.
تلفان، عناد احمد المهداوى (1993) أساسيات الكيمياء الحيوية، دار الكتب الوطنية بنغازى، الطبعة الأولى.
زايد، عبد الله عبد الرحمن وعبد الرحمن، خوجلي مبارك (1995) علم وظائف الأعضاء العام (الفيزيولوجيا العامة)، منشورات جامعة عمر المختار، البيضاء، الطبعة الأولى، 299-378.
زايد، عبد الله عبد الرحمن ومحمد، خلف توني (1998) علم وظائف الأعضاء الغدد الصماء والهرمونات، منشورات جامعة عمر المختار، الطبعة الأولى، 51-63.
فتحي، عبدالعزيز عفيفي (2002) أسس علم السموم، دار الفجر للنشر والتوزيع، القاهرة، الطبعة الأولى.
محمد، مدحت حسين خليل (1997) علم الغدد الصماء، مكتبة المدينة، العين، الإمارات العربية المتحدة، جامعة الازهر، 177-233.
محمد، مدحت حسين خليل (2005) فسيولوجيا الحيوان، دار الكتاب الجامعي، العين، الإمارات العربية المتحدة، الطبعة الثانية، 429-463.
محمد، مدحت حسين خليل (2012) فسيولوجيا الإنسان، دار الكتاب الجامعي، العين، الإمارات العربية المتحدة، الطبعة الأولى، 379-446.
محيي الدين، خيرالدين، وليد، حميد يوسف وسعد، حسين توحلة (1990) فسلجة الغدد الصم والتكاثر في الثدييات والطيور، دار الحكمة للطباعة والنشر، الموصل، العراق.
الكبيسي، خالد (2002) الكيمياء الحيوية – العلوم الطبية المساعدة، دار وائل للنشر والتوزيع، عمان، الأردن، الطبعة الأولى.
غايتون، أ.س وهول، ج.ي (1997) المرجع في الفسيولوجيا الطبية، ترجمة صادق الهلالي، منظمة الصحة العالمية، مكتب الشرق الأوسط، الطبعة التلسعة.
Abraham, G., Gottschalk, J. and Ungemach, F.R. (2005) Evidence for ototopical gluco-corticoid – induced decrease in hypothalamic – pituitary – adrenal axis response and liver function. Endocrinology, 146, 3163-3171. DOI: https://doi.org/10.1210/en.2005-0080
Ali, R.j., Amlal, H., Burnham, C.e. and Soleimani, M. (2000) Glucocorticoids anhance the axpression of the basolateral Na+ : Hco3_ cotransporter in renal proximal tubules. Kidney Int, 57, 1063-1071. DOI: https://doi.org/10.1046/j.1523-1755.2000.00933.x
Bancroft, J.D. and Gamble, M. (2002) Theory and practice of histological techniques. Churchill Livingston. Edinburgh. London & New York, 5th edition.
Bart, A.B., Portman, N. and Macsueer, R.N.M. (2002) Liver pathology. Churchill livincstone., 828-829.
Bogusz, M. (1968) Activity of certain enzymatic systems in agricultural workers exposed to organophosphorus insecticides. Pol. Tyg. Lek., 23, (21), 787-789.
Carlton, w.w. and McGavin, M.D. (1995) Special veterinary pathology. Philadelphia, New York, 2nd edition.
Center, S.A., Slater, M.R., Manwarren, T. and Prymak, K. ( 1992) Diagnostic efficacy of serum alkaline phosphatase and γ-glutamyltransferase in dogs with histologically confirmed hepatobiliary disease: 270 cases (1980–1990). J. Am. Vet. Med. Assoc., 201, 1258–1264.
Crossmon, G. (1937) A modification of Mallory connective tissue stain with discussion of the principle involved. Ant. Rec., 69, 33-38. DOI: https://doi.org/10.1002/ar.1090690105
DeNovo, R.C. and Prasse, K.W. (1983) Comparison of serum biochemical and hepatic functional alterations in dogs treated with corticosteroids and hepatic duct ligation. Am. J. Vet. Res., 44, 1703–1709.
Elshennawy, W.W. and Abo El-Wafa, H.R. (2011) Histological and ultrastructural changes in mammalian testis under the effect of hydrocortisone. J. Am. Sci., 7, (9), 38-48.
Gedde, D.M. (1992) Inhaled corticosteroids: benefits and risks. Thorax., 47, 404 – 407. DOI: https://doi.org/10.1136/thx.47.6.404
Hadley, S.P., Hoffmann, W.E., Kuhlenschmidt, M.S., Sanecki, R.K. and Dorner, J.L. (1990) Effect of glucocorticoids on alkaline phosphatase, alanine aminotransferase, and γ-glutamyltransferase in cultured dog hepatocytes. Enzyme., 43, 89–98. DOI: https://doi.org/10.1159/000468711
Hartgens, F., Kuipers, H., Wijnen, J.A. and Keizer, H.A. (1996) Body composition, cardiovascular risk factors and function in long-termandrogenic-anabolic steroids using bodybuilders three monthe after drug withdrawal. Sport Med., 17,(6), 429-433. DOI: https://doi.org/10.1055/s-2007-972873
Kawai, K., Tamaki, A. and Hirohata, K. (1985) Steroid – induced accumulation of lipid in the osteocytes of the rabbit femoral head: a histochemical and electron microscopic study. J. Bone and Joint surgery., 67, (5), 755-763. DOI: https://doi.org/10.2106/00004623-198567050-00010
Lipworth, B.J. (1999) Systemic adverse effects of inhaled corticosteroid therapy: a systematic review and meta-analysis. Arch. Intern. Med., 59, 941–955. DOI: https://doi.org/10.1001/archinte.159.9.941
Molano, F., Saborido, A., Delgado, J., Moran, M. and Megias, A. (1999) Rat lives lysosomal and mitochondrial activities are modified by anabolic androgenic steroids. Med. Sci. Sports Exerc., 31, (2), 243-250. DOI: https://doi.org/10.1097/00005768-199902000-00007
Negi, C.S. (2009) Introduction to endocrinology. asoke k. ghosh, PHI iearning private limited, New Delhi. Chapter nine, 144-170.
Ott, L. (1984) An introduction to statistical methods and Data Analysis. Duxburg Press, Boston, USA, 2nd edition.
Rutgers, H.C., Batt, R.M., Vaillant, C. and Riley, J.E. (1995) Subcellular pathologic features of glucocorticoid-induced hepatopathy in dogs. Am. J. Vet. Res., 56, 898–907.
Sanecki, R.K., Hoffmann, W.E., Gelberg, H.B. and Dorner, J.L. (1987) Subcellular location of corticosteroid-induced alkaline phosphatase in canine hepatocytes. Vet. Pathol., 24, 296–301. DOI: https://doi.org/10.1177/030098588702400402
Solter, P.F., Hoffmann, W.E., Chambers, M.D., Schaeffer, D.J. and Kuhlenschmidt, M.S. (1994) Hepatic total 3α-hydroxy bile acids concentration and enzyme activities in prednisone-treated dogs. Am. J. Vet. Res., 55, 1086–1092.
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