Physiological and Histological Studies on the Effect of Hydrocortisone on Rabbit Liver


  • Zainab Amma Shoaib Al Haddad Department of Zoology, Faculty of Science, Omar Al-Mukhtar University, Al-Bayda, Libya
  • Abdel Salam Musa Boulaj Department of Zoology, Faculty of Science, Omar Al-Mukhtar University, Al-Bayda, Libya
  • Saad Mohammed Gharbaw Department of Zoology, Faculty of Science, Omar Al-Mukhtar University, Al-Bayda, Libya



Hydrocortisone, Liver, Liver Enzymes, Rabbits, Glucocorticoids


The aim of This study was to test the effect of The drug hydrocortisone sodium succinate on blood picture, serum components and liver tissues in white rabbits.The experiment included 30 male Rabbits ranged between 1500-2500g. They were divided into 4 control and treated groups, for different periods of time, depending on the duration of injection. After treatment of the rabbits with a  hydrocortisone dose of 10 mg/kg daily for one week and two weeks frequent urination was observed. Swelling of The urinary bladder and congestion of liver was also noticed after slaughtering of the animal. An increase in the amount of adipose tissue around the liver and kidneys in the gradually treated group was observed. Treatment of rabbits with the drug for a week did not lead to change in the weights. Non significant increase occurred in the weights of rabbits treated for two weeks and treated gradually, but in sudden treatmed group a non significant decrease in rabbits weights was recorded in comparison with their weights before injection. The treatment for a week and two weeks led to a significant increase in the levels of the following liver enzymes: aspartate amino transferase(AST), alanin amino transferase (ALT) and alkaline phosphatase enzymes(ALP). The values of these enzymes remained high in both suddenly and gradually treated groups similar to that of the two weeks treated group. Results of biochemical tests showed significant increase in the level of glucose in rabbits treated for a week, but the treatment for two weeks did not lead to a significant increase. In the suddeny treated group, The parameters did not change than that present in the two weeks treated rabbits. While, In the gradually treated group, the blood glucose level returned to the normal value. The histological examination of the livers of rabbits treated for a week revealed congestion and dilatation of central veins. Also in some sections there was hemolysis within the central veins. The same group also showed that there were intracytoplasmic vacuoles in some liver cells. In two weeks treated rabbits these pathological changes became more obvious, in addition, dilataion of hepatic sinusoids occurred. In rabbits in which the treatment was stopped suddenly; the central veins remained dilated, but the congestion and hemolysis were decreased. The dilataion of hepatic sinusoids and vacuolation of hepatocytes were also decreased. As for the rabbits that their treatment was gradually stopped most of the central veins returned to their normal size, but remained full of hemolytic erythrocytes. Hepatic sinusoids also returned to their normal size, but the intracytoplasmic vacuoles remained distributed in the liver cells with a reductioin in their quantity and size. Histochemical examination showed decreased reactivity of liver cells with periodic acid Schiff (PAS) stain in both groups. There was also a decrease in the distribution of glycogen granules in the hepatocytes of the treated rabbits; were most of the cell parts appeared devoid of these granules.


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How to Cite

Al Haddad ز. ع. ش., Boulaj ع. ا. م. . ., & Gharbaw س. م. . (2013). Physiological and Histological Studies on the Effect of Hydrocortisone on Rabbit Liver. Al-Mukhtar Journal of Sciences, 28(2), 96–117.



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