Effects of Different Types of Honey on Wound Healing in rats
Keywords:Honey, Induced skin wound, wound healing, Rats
One hundred and twenty healthy female Sprague-Dawley rats weighing between 300 to 350g were used in this study over a 20-day period. They were allocated randomly into 4 groups of 30 animals each. After the creation of 2cm x 2cm open wound, Group I was control treated with Gentamycin ointment. Groups II, III and IV were treated with Different Types of Honey. On application, the honey was well accepted by the animals without any adverse reaction. On clinical examination, Groups II, III and IV showed bright beefy red color granulation tissue with angiogenesis when compared to Groups I. On bacteriological examination, Staphylococcus aureus, Pseudomonas, Escherichia coli, Proteus and Klebsiella species were isolated from all the groups. Mean percentage of epithelialistion, wound contraction and total healing were significantly better in Group IV (P<0.05).
Honey can be a better wound healing biomaterial in rats. It can be used as a less expensive skin substitute in order to stimulate and promote wound healing in animals.
Aljady, A., Kamaruddin, M., Jamal, A., & Mohd-Yassim, M. (2000). Biochemical study on the efficacy of Malaysian honey on inflicted wounds: an animal model. Medical Journal of Islamic Academy of Sciences, 13(3), 125-132.
Altomare, M., Nascimento, A. P., Romana-Souza, B., Amadeu, T. P., & Monte-Alto-Costa, A. (2009). Ultrasound accelerates healing of normal wounds but not of ischemic ones. Wound repair and regeneration, 17(6), 825-831. DOI: https://doi.org/10.1111/j.1524-475X.2009.00542.x
Arul Jothi, N., Thilagar, S., Khaled, M. A., Thevi, S. G., Lau, S., and Sastry, T. (2006). Clinical evaluation of keratin-gelatin composite film for wound healing in cats. Indian Journal of Veterinary Surgery, 27(2): 108-110.
Ashraf, A. H., & Khaled, M. A. H. (2008). Diagnosis of joint affection in camels by studies on synovial fluid which give the degree of affections & response to treatment. Al-Mukhtar Journa of Sciences, 20(1), 58-72.
Baie, S. H., & Sheikh, K. A. (2000). The wound healing properties of Channa striatus-cetrimide cream—tensile strength measurement. Journal of ethnopharmacology, 73(1), 15-30. DOI: https://doi.org/10.1016/S0378-8741(00)00253-1
Ballard, K., & Baxter, H. (2000). Developments in wound care for difficult to manage wounds. British journal of nursing, 9(7), 405-412. DOI: https://doi.org/10.12968/bjon.2000.9.7.6319
Bhatia, P., & Ashwath, N. (2002). Development of a rapid method for micropropagation of a new pineapple [Ananas comosus (l.) Murr.] clone,'Yeppoon gold'. Paper presented at the International Symposium on Tropical and Subtropical Fruits 575. DOI: https://doi.org/10.17660/ActaHortic.2002.575.11
Bohling, M. W., Henderson, R. A., Swaim, S. F., Kincaid, S. A., & Wright, J. C. (2004). Cutaneous wound healing in the cat: a macroscopic description and comparison with cutaneous wound healing in the dog. Veterinary Surgery, 33(6), 579-587. DOI: https://doi.org/10.1111/j.1532-950X.2004.04081.x
Cheng, B., Fu, X., Sheng, Z., Gu, X., Sun, T., & Sun, X. (1999). The effect of basic fibroblast growth factor on myofibroblasts and its significance on wound healing. Zhonghua yi xue za zhi, 82(17), 1187-1191.
Cockbill, S. (2002). Wounds the healing process. Hospital pharmacist-london, 9(9), 255-260.
Consultation, W. (1999). Definition, diagnosis and classification of diabetes mellitus and its complications: Part.
Druecke, D., Lamme, E. N., Hermann, S., Pieper, J., May, P. S., Steinau, H. U., & Steinstraesser, L. (2004). Modulation of scar tissue formation using different dermal regeneration templates in the treatment of experimental full-thickness wounds. Wound repair and regeneration, 12(5), 518-527. DOI: https://doi.org/10.1111/j.1067-1927.2004.012504.x
Dunford, C., Cooper, R., Molan, P., & White, R. (2000). The use of honey in wound management. Nursing Standard (through 2013), 15(11), 63. DOI: https://doi.org/10.7748/ns2000.11.15.11.63.c2952
Hamad, A. M., & Taha, R. M. (2008). Effect of sequential subcultures on in vitro proliferation capacity and shoot formations pattern of pineapple (Ananas comosus L. Merr.) over different incubation periods. Scientia Horticulturae, 117(4), 329-334. DOI: https://doi.org/10.1016/j.scienta.2008.05.009
Hosgood, G. (2003). Wound repair and specific tissue response to injury. Textbook of Small Animal Surgery. 3rd ed. Philadelphia: WB Saunders, 1, 327-329.
James, A., & Bayat, A. (2003). basic plastic surgery techniques and principles: chronic wound management. Student Br Med J, 11, 406-407. DOI: https://doi.org/10.1136/sbmj.0311406
Kawai, K., Suzuki, S., Tabata, Y., Ikada, Y., & Nishimura, Y. (2000). Accelerated tissue regeneration through incorporation of basic fibroblast growth factor-impregnated gelatin microspheres into artificial dermis. Biomaterials, 21(5), 489-499. DOI: https://doi.org/10.1016/S0142-9612(99)00207-0
Khaled, M., Jalila, A., Kalthum, H., Noordin, M., & Saleh, W. A. (2014). Collagen-Calcium Alginate Film Dressing with Therapeutic Ultrasound to Treat Open Wound in Rats. Research Journal of Biological Sciences, 9(2), 57-61.
Khaled, M. A. H., & Ashraf, A.-h. H. (2008). Unusual foreign bodies cause obstruction of the esophagus in dogs. Al-Mukhtar Journa of Sciences, 19(1), 51-61.
Khaled, M. A. H., Asma, S. E., & Almahdi, A. K. H. (2016). Clinical Evaluation of Collagen-Calcium Alginate Film along with Therapeutic Ultrasound Massage for Wound Healing in Cats. Acta Biologica Malaysiana, 5(2&3), 49-52.
Kumar, M. S., Sripriya, R., Raghavan, H. V., & Sehgal, P. V. (2006). Wound Healing Potential of Cassia fistula on Infected Albino Rat Model. Journal of Surgical Research, 131(2), 283-289. DOI: https://doi.org/10.1016/j.jss.2005.08.025
Mali, S., Grossmann, M. V. E., García, M. A., Martino, M. N., & Zaritzky, N. E. (2006). Effects of controlled storage on thermal, mechanical and barrier properties of plasticized films from different starch sources. Journal of food engineering, 75(4), 453-460. DOI: https://doi.org/10.1016/j.jfoodeng.2005.04.031
Michiyo, M., Toshito, I., & Shigehiko, S. (2005). Effrcts of bFGFgelatin composite film incorporated into gelatin sheet on wound healing. J. Biomat. sci. poly. ed, 16(7), 893-907. DOI: https://doi.org/10.1163/1568562054255709
Molan, P., & Betts, J. (2004). Clinical usage of honey as a wound dressing: an update. Journal of wound care, 13(9), 353-356. DOI: https://doi.org/10.12968/jowc.2004.13.9.26708
Molan, P. C. (1996). Honey as an antimicrobial agent. Bee Products, 3, 27-37. DOI: https://doi.org/10.1007/978-1-4757-9371-0_3
Molan, P. C. (1999). The role of honey in the management of wounds. Journal of wound care, 8(8), 415-418. DOI: https://doi.org/10.12968/jowc.1918.104.22.168904
Moore, O. A., Smith, L. A., Campbell, F., Seers, K., McQuay, H. J., & Moore, R. A. (2001). Systematic review of the use of honey as a wound dressing. BMC complementary and alternative medicine, 1(1), 2. DOI: https://doi.org/10.1186/1472-6882-1-2
Pope, E. R. (1993). skin healing .In: Disease mechanisms in small animal surgery. 2nd ED Philadelphia, London, 152-155.
Probst, C. W. (2003). Wound healing and specific tissue regeneration. In. Texbook of small animal surgery. 3rd Ed. Saunders Company. Philadelphia, 1, 53-63.
Rafi, M. E. A., Ashraf, A.-h. H., & Khaled, M. A. (2008). Foreign bodies in the rumen of the sheep and goats. Al-Mukhtar Journa of Sciences, 19(1), 38-50.
Rangaraj, A., Harding, K., & Leaper, D. (2011). Role of collagen in wound management. Wounds UK, 7(2), 54-63.
Richard, J. L., Daures, J. P., Richard, C. P., Vannereau, D., & Boulot, I. (2000). Of mice and wound reproducibility and accuracy of a novel planimetery program for measuring wound area. Wounds, 12(6), 148-154.
Schäffer, M. R., Tantry, U., & Barbul, A. (2004). Wound fluid inhibits wound fibroblast nitric oxide synthesis. Journal of Surgical Research, 122(1), 43-48. DOI: https://doi.org/10.1016/j.jss.2004.05.009
Schmidt, R., Chung, L., Andrews, A., & Turner, T. (1992). Hydrogen peroxide is a murine (L929) fibroblast cell proliferant at micro-to nanomolar concentrations. Paper presented at the Second European Conference on Advances in Wound Management, Harrogate, October 20-23.
Swaim, S., Bradley, D., Spano, J., McGuire, J., Hoffman, C., & Trachy, R. (1993). Evaluation of multipeptide-copper complex medications on open wound healing in dogs. The Journal of the American Animal Hospital Association, 29, 519-525.
Takehara, K. (2000). Growth regulation of skin fibroblasts. Journal of dermatological science, 24(1), 70-77. DOI: https://doi.org/10.1016/S0923-1811(00)00144-4
Tonnesen, M. G., Feng, X., & Clark, R. A. F. (2000). Angiogenesis in wound healing. Journal of Investigative Dermatology Symposium Proceedings, 5(1), 40-46. DOI: https://doi.org/10.1046/j.1087-0024.2000.00014.x
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