Isolation and identification of seed borne fungi on dry bean and determination of their location on seed coats


  • Ibtisam, M. Lashger Plant Protection Department, Faculty of Agriculture, Omar AL-Mukhtar University
  • Zahra,I. El gali Plant Protection Department, Faculty of Agriculture, Omar AL-Mukhtar University
  • Hanan A Khalifa Department of Botany, Faculty of Arts & Sciences, El-Gubba, Omar AL-Mukhtar University



Dry seed of bean, EL-Biada market, Seed-borne fungi, seed coats


This study was conducted at the Faculty of Agriculture, University of Omar AL-Mukhtar, during the growing seasons 2014-2015, aiming to justify many objectives including  isolation and identification of seed-borne fungal pathogen on dry bean (Phaseolus  vulgaris L.). The study also aimed to determine the location of pathogens on seed parts. The Seed samples were collected from the local market and underwent an isolation process after the surface sterilization of seeds to know the most important plant pathogens carried on beans seeds and determine their location and estimate their severity.  Our results indicated that ten pathogenic fungi were isolated  and  identified as (Alternaria sp, Aspergillus niger, Aspergillus flavus, Penicillium sp ,Fusarium solani, Fusarium oxysporium ,Chaetumium sp,Rhizoctonia sp,Cladosporium sp, Botrytis cinearea). Results also emphasized the presence of fungi   Rhizoctonia sp, Chaetumium sp in the seed coat, and Aspergillus sp ,Penicillium sp in embryio, While fungus Fusarium solani, ,Cladosporium sp, F. oxysporium , Alternaria sp  ,Penicillium sp were noted in Cotyledons . 


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أبو بكر، نجاح سليمان . (2000) . عزل وتعريف الممرضات الفطرية المحمولة على بذور بعض الأنواع البقولية وطرق مكافحتها، رسالة ماجستير- قسم وقاية النبات ,كلية الزراعة– جامعة عمر المختار- البيضاء،ليبيا.93صفحة.

الجالي، زهرة إبراهيم، (1996). تلوث بذور بعض المحاصيل بسموم الإفلاتوكسين في منطقة الجبل الأخضر. رسالة ماجستير- قسم وقاية النبات,كلية الزراعة- جامعة عمر المختار. 196 صفحة

الجالي. زهرة إبراهيم . (2012). دراسات على انتقال الفطر Fusarium solani of phaseoli.في بذور الفاصوليا- المجلة الليبية لوقاية النبات.(2):63 -75

عبدالعالي، عازة علي. (2016). عزل وتعريف فطريات أعفان قرون البازلاء ومكافحتها ببعض الطرق الفيزيائية. رسالة ماجستير. قسم وقاية النبات, كلية الزراعة - جامعة عمر المختار95 صفحة .

محمد، نوارة علي، فوزية مفتاح بونصيرة، نجوى عبدالستار إبراهيم (2013). حصر الفطريات المحمولة في بذور البقوليات وفصل سمومها. مجلة المختار العدد 28 (2) 127-136. DOI:

ميخائيل، سمير. (1992). أمراض البذور. منشأة المعارف بالإسكندرية، جلال حزي وشركاه 283 صفحة.

نيرجارد (1977). أمراض البذور المجلد الأول ترجمة عوض محدم عبدالرحيم ومحمد عبدالجواد العوشار .(1995). منشورات جامعة عمر المختار. 925 صفحة .

Abigail, J. (2005). Soil biology basics – Profitable and sustainable Primary Industries - Soil fungiNew South Wales Department of Primar Industries.

Alexopoulos, C. J., Mime, C. W. and Blackwell, M. (1996). Introductory of Mycology. 4th Edition. John Wiley and Sons.880pp.

Barnett, H. L. and Hunter, B. B. (1972). Illustrated Genera of Imperfect Fungi. Burgess Publication Ltd. St. Paul, Minnesota, USA, p-241pp.

Domsch, K. and Games, W.(1980). Compendium of soil fungi vol.1and vol 2.Academic press, London 859 pp

Domijan, M. Peracia, M. Zlender, V. Cvjetkovic, B. Jurjevic, Z., Topolovec - Pintaric, S. and Ivic, D. (2005). Seed-born fungi and ochratoxin A contamination of drybeans (Phaseolus vulgaris L.) in the Republic of Crotia. Food and Chemical Toxicology: 43:, 427- 432. DOI:

El-Mougy, S.N. Nadia, G. E. and Abdel-Kader, M. M. (2007). Control of wilt and root rot incidence in (phaseolus Vulgaris L.) By some plant volatile compounds. J., Plant Protect .Res., 47(3) 255- 265.

El-Gali, Z. I. (2003). Histopathological and biochemical studies on Phaseolus vulgaris seeds infected by some seed-borne fungi. Ph.D. Thesis. Alexandria University. 300 pp.

Giovannetti, M. and Mosse, B. ( 1980). An evaluation of techniques of measuring vesicular arbuscular mycorrhizal infection in roots . New Phytology 84 489-500. DOI:

Gonzalez, H. Mortinez, E. Pacin, A. and Resnik, L. (1999). Relationship between Fusarium graminearum and Alternaria alternata contamination and deoxinivalenol occurrence on Argentinian durum wheat. Mycopathologia, 144:97-102. DOI:

Hemeda, A. H. Wafaa, T. S. and Abo-Shosha, S. S. (2001). Quantitative changes in amino acids contents in relation to natural fungal infection of some legumenous seeds. J. Agric. .24:415-424.

Islam, S. M. Masum, M. M. I. and Fakir, M. G. (2009). Prevalence of seed-borne Fungi in sorghum of different locations of Bangladesh. Scientific Research and Essay.4(3):175-179.

ISTA (International Seed Testing Association). (1980). International rules for seed testing. Rules Amendments. Seed Sci and Tech. 29(2):1-127.

Javaid, A. and Anjum, T. (2006). Fungi associated with seeds of some economically important crops in Pakistan- a review. PJST 1:55-61.

Lokhande, S. B. More, W. D. and Shinde, P.A. (1986). Fungi associated with common bean .Journal of the Maharashtra-Agricultural University 11:275-278.

Narayan, M, G.and Ayodhya, D. K. (2013). Study of seed borne fungi of different legumes. Triends in Life Science.TLS (2):1-8.

Sabry, Y. M., Mahmoud, M. H.Hosseny,E. Ali, H. A. (2013). seed borne fungal pathogens associated with common bean ( Phaseolus vulgaris l) seeds and their impact on germination. journal of Enviromental Studies(11):19-26. DOI:

Shakir, S. Mirza, H. Sahi, T. and Ahmed, F. (1995). Detection of seed-borne fungi associated with sponge gourd (Luffa cylindrical,L) their location in different seed components and their control,.Pak.J.Phytopathol,.7:140-144.

Szafirowska, A. (2013). Analiza dostępności i jakości ekologicznego materiału siewnego warzyw na rynku krajowym. Journal of Research and Applications in Agricultural Engineering, 58(4): 174-178.

Tosh, S. and Yada, S. (2010). Dietary fibres in pulse seeds and fractions: Characterization, functional attributes, and applications. Food Research International, 43: 450-460. DOI:

Tylkowska, K. Turek, M. and Prieto, R. (2010). Health germination and vigour of common bean seeds in relation to microwave irradiation. Phytopathologia, 55: 5–12.

Wondołowska,A. and Grabowska, A. (2003). Wpływ terminu i sposobu zbioru fasoli zwyczajnej (Phaseolus vulgaris L.)na przyrost masy nasion i ich wartość użytkową. Zastosowania metod statystycznychw badaniach naukowych II. Obtained from: Downloadwplywparametrow.pdf.

Wright, J. ( 2007). Beans: A History/Food: The History of Taste. History Today 57(11):64-65 DOI:




How to Cite

Lashger, I. M., El gali , Z., & Khalifa, H. A. (2018). Isolation and identification of seed borne fungi on dry bean and determination of their location on seed coats. Al-Mukhtar Journal of Sciences, 33(1), 12–18.



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