Identification and Classification of Libyan Rosmarinus Officinalis Essential Oil Components by GC-MS and Predication of Its Antioxidant Activity


  • Wessal Hassan El-Ageeli Department of Food Science and Technology, Faculty of Agriculture, Omar Al-Mukhtar Univer-sity, Beida, Libya
  • Bubaker Bubaker M.B Hamed Al-Mansori Department of Food Science and Technology, Faculty of Agriculture, Omar Al-Mukhtar Univer-sity, Beida, Libya
  • Seham Hamad Mohammed Alsagheer Department Of Chemistry, Benghazi University, Almarj City, Libya,Department Of Chemistry, Benghazi University, Almarj City, Libya
  • Faheem A. F. Ben-Khayal Department of Food Science and Technology, Faculty of Agriculture, Omar Al-Mukhtar Univer-sity, Beida, Libya



Rosmarinus Officinalis, essential oils, GC-MS analysis of essential oil, antioxidants


The aim of this investigation is identification and classification of Rosmarinus Officinalis essential oil components by GC-MS and prediction of their antioxidant activity.     GC-MS analysis of Libyan Rosemary essential oil indicated that in total, 44 compounds were detected comprising 98.3% of compounds in the oil. The results also revealed that the oil contained some constituents with great antioxidant activity such as eugenol, (0.04%), diethylphalate (0.28%), myrtenol (0.46%), γ-Terpinene (2.2%), α – Terpenolene (1.96%), with total percentage of (4.94%) which was considered to be very small. However, about the half of the essential oil contained of constituents with very weak antioxidant activity. These include trans-β-caryophinen (2.2%), α- humulene (0.34%), 1.8- cineol (6.15%), 1.8 – cineol isomer (11.8%), linalool (3.29%), 4-terpinol (5.65%), 1-α – terpine (7.93%), isoborneol (0.29%), perillaldehyde (0.09%), β- citronellal (0.03%), α – pinene (3.4%), comphen (3.04%) and β- pinene(4.21 %), our result shows that the essential oil of Libyan Rosemary might have a weak antioxidant activity.


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Alvarez, María & Ortega Ramirez, Luis & Silva-Espinoza, Brenda & Aguilar, Gustavo & Ayala-Zavala, J. Fernando. (2019). Antimicrobial, antioxidant, and sensorial impacts of oregano and rosemary essential oils over broccoli florets. Journal of Food Processing and Preservation. DOI:

Bakkali, F., Averbeck, S., Averbeck, D. and Waomar, M. (2008). Biological effects of essential oils - A review. Food and Chemical Toxicology, 46, 446-475. DOI:

Bozin, B., Mlmica-Dukic, N., Samojlik, I. and Jovin, E. (2007). Antimicrobial and antioxidant properties of rosemary and sage (Rosmarinus officinalis L. and Salvia officinalis L., lamiaceae) essential oils. Journal of Agricultural and Food Chemistry, 55, 7879-7885. DOI:

Burt, S. 2004. Essential oils: their antibacterial properties and potential applications in foods - a review. International Journal of Food Microbiology, 94, 223-253. DOI:

Daferera, D. J., Ziogas, B. N. and Polissiou, M. G. 2003. The effectiveness of plant essential oils on the growth of Botrytis cinerea, Fusarium sp and Clavibacter michiganensis subsp michiganensis. Crop Protection, 22, 39-44. DOI:

Fitsiou, Eleni & Anestopoulos, Ioannis & Chlichlia, Katerina & Galanis, Alex & Kourkoutas, Yiannis & Panayiotidis, Mihalis & Pappa, Aglaia. (2016). Antioxidant and Antiproliferative Properties of the Essential Oils of Satureja thymbra and Satureja parnassica and their Major Constituents. Anticancer Research. DOI:

Hosni, K., Hassen, I., Chaabane, H., Jemli, M., Dallali, S., Sebei, H. and Casabianca, H. 2013. Enzyme-assisted extraction of essential oils from thyme (Thymus capitatus L.) and rosemary (Rosmarinus officinalis L.): Impact on yield, chemical composition and antimicrobial activity. Industrial Crops and Products, 47, 291-299. DOI:

Jafri, S. M. H. and El-Gadi, A. 1985. Flora of Libya., Tripoli, Libya, Department of Botany, Al- Faateh Univ.

Jordan, M. J., Martinez, R. M., Goodner, K. L., Baldwin, E. A. and Sotomayor, J. A. (2006). Seasonal variation of Thymus hyemalis Lange and Spanish Thymus vulgaris L. essential oils composition. Industrial Crops and Products, 24, 253-263. DOI:

Lahlou, M. and Berrada, R. (2003). Conmposition and niticidal activity of essential oils of three chemotypes of Rosmarinus officinalis L. acclimatized in Morocco. Flavour and Fragrance Journal, 18, 124-127. DOI:

Lee, S. J., Umano, K., Shibamoto, T. and Lee, K. G. (2005). Identification of volatile components in basil (Ocimum basilicum L.) and thyme leaves (Thymus vulgaris L.) and their antioxidant properties. Food Chemistry, 91, 131-137. DOI:

Mata, A. T., Proenca, C., Ferreira, A. R., Serralheiro, M. L. M., Nogueira, J. M. F. and Araujo, M. E. M. (2007). Antioxidant and antiacetylcholinesterase activities of five plants used as Portuguese food spices. Food Chemistry, 103, 778-786. DOI:

Miura, K., Kikuzaki, H. and Nakatani, N. (2002). Antioxidant activity of chemical components from sage (Salvia officinalis L.) and thyme (Thymus vulgaris L.) measured by the oil stability index method. Journal of Agricultural and Food Chemistry, 50, 1845-1851. DOI:

Mueller-Riebau, F. J., Berger, B. M., Yegen, O. and Cakir, C. (1997). Seasonal variations in the chemical compositions of essential oils of selected aromatic plants growing wild in Turkey. Journal of Agricultural and Food Chemistry, 45, 4821-4825. DOI:

Nieto, Gema & Id, Gaspar & Ros, Gaspar & Castillo, Julián. (2018). medicines Antioxidant and Antimicrobial Properties of Rosemary (Rosmarinus officinalis, L.): A Review. Medicines. DOI:

Okoh, O. O., Sadimenko, A. P. and Afolayan, A. J. 2010. Comparative evaluation of the antibacterial activities of the essential oils of Rosmarinus officinalis L. obtained by hydrodistillation and solvent free microwave extraction methods. Food Chemistry, 120, 308-312. DOI:

Ojeda, Adriana & Van Baren, Catalina & Elechosa, Miguel & Juárez, Miguel & Moreno, Silvia. (2013). New insights into antibacterial and antioxidant activities of rosemary essential oils and their main components. Food Control. 31. 189–195. 10.1016/j.foodcont.2012.09.022. DOI:

Ormancey, X., Sisalli, S. and Coutiere, P. 2001. Formulation of essential oils in functional perfumery Parfums, Cosmetiques, Actualites., 157, 30-40.

Panizzi, L., Flamini, G., Cioni, P. L. and Morelli, I. (1993). Composition and antimicrobial properties of essential oils of four Mediterranean Lamiaceae. Journal of Ethnopharmacology, 39, 167-170. DOI:

Ravid, U. and Putievsky, E. (1986). Carvacrol and thymol chemotypes of East Mediterranean wild Labiatae herbs. In: Brunke, E. J. (ed.) Progress in Essential Oil Research. New York: de Gruyter, Berlin.

Ruberto, G. and Baratta, M. T. (2000). Antioxidant activity of selected essential oil components in two lipid model systems. Food Chemistry, 69, 167-174. DOI:

Stahl-Biskup, E. (2002). Essential oil chemistry of the genus Thymus – a global view. In: Stahl-Biskup, E. and Sáez, F. (eds.) The genus thymus. New York: CRC Press

Soliman, F. M., El-Kashoury, E. A., Fathy, M. M. and Gonaid, M. H. (1994). Analysis and biological activity of the essential oil of Rosmarinus officinalis L. from Egypt. Flavour and Fragrance Journal, 9, 29-33. DOI:

Tomeia, P. E., Cionib, P., Flaminib, G. and Stefanic, A. (1995). Evaluation of the Chemical Composition of the Essential Oils of Some Lamiaceae from Serrania de Ronda (Andaluçia, Spain). Journal of Essential Oil Research, 7, 279-282. DOI:

Van De Braak, S. a. a. J. and Leijten, G. C. J. J. (1999). Essential oils and oleoresins: a survey in the Netherlands and other major markets in the European Union. CBI, Centre for the Promotion of Imports from Developing Countries, pp. 116.

Villiere, A., Viau, M., Bronnec, I., Moreau, N. and Genot, C. (2005). Oxidative stability of bovine serum albumin- and sodium caseinate-stabilized emulsions depends on metal availability. Journal of Agricultural and Food Chemistry, 53, 1514-1520. DOI:

Wang, W., Wu, N., Zu, Y. G. and Fu, Y. J. (2008). Antioxidative activity of Rosmarinus officinalis L. essential oil compared to its main components. Food Chemistry, 108, 1019-1022. DOI:

Youdim, K. A., Deans, S. G. and Finlayson, H. J. )2002(. The antioxidant properties of thyme (Thymus zygis L.) essential oil: an inhibitor of lipid peroxidation and a free radical scavenger. Journal of Essential Oil Research, 14, 210-215. DOI:




How to Cite

El-Ageeli, W. H. ., Al-Mansori, B. B. M. H. ., Alsagheer, S. H. M. ., & Ben-Khayal , F. A. F. . (2020). Identification and Classification of Libyan Rosmarinus Officinalis Essential Oil Components by GC-MS and Predication of Its Antioxidant Activity. Al-Mukhtar Journal of Sciences, 35(3), 184–192.



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