The effect of papavarine on contractile and enzyme Activity and on Oxygen consumption of ileal smooth muscle of laboratory mice


  • Khalid H. M. Saad Department of Zoology, College of Arts and Sciences, Omar Mukhta University, Libya



Papavarine, Enzyme Activity , Oxygen consumption, Ileal smooth muscle, Laboratory mice


 Ileal smooth muscle of the laboratory mice exhibits regular spontaneous rhythmic contractions. The Different concentrations of papavarine exert different effect on ileal smooth muscle of the laboratory mice. Its found  that low concentrations (0.001- 0.01)Mm where with  effect while the moderate and high concentrations ( 0.05-02) mM caused instant relaxation of normal activity ,the inhibitory effect of the drug was dose dependent.Also the high concentration of papaverine that caused instant relaxation of normal activity caused gradual decline of acetylcholine – induced tonic contracture of laboratory mice smooth muscle. The presence of the drug in the normal saline reduces largely the response of the muscle to acetylcholine. It has been found that muscle incubated in a. Ca+2 – free physiological solution failed to restore normal spontaneous activity to the re- introduction of calcium.also it has been found that the Ca++ taken by muscle in the presence of the drug was much higher than in un treated muscle.   This study showed a clear relationship between the inhibitory  effect of the  drug on the muscle contractions activity and on its effect on oxygen consumption.The presence of papaverine in the incubation medium inhibits  the enzyme activity in isolated mitochondria and membrane vesicles from ileal smooth muscle of laboratory mice and the effect was dose dependent  as well as  Inhibition of oxygen consumption in these fraction.


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Al-Badran, I , A(1989). The effect of Quinine on the mechanical activity 1- Al-Badran, I , A and oxygen consumption of ileal smooth muscle of Golden Hamester and Laboratory Mice.Msc. Salahaddin, Univ.

Altura, B, M ,and Altura, B, T, (1974). Influence of magnesium on drug-induced contraction on ion content in rabbit aorta. Am. J. physiol.(London), 220:938-944. DOI:

Andersson, R, G ,(1972).cyclic-AMP and Ca+2 in relaxation in intestinal smooth muscle. Nature New,Biol.,238,119-122. DOI:

Atsumi, A, (1978). Sarcoplasmic Reticulum and intracellular calcium Localization at rest and during contraction in mytilus smooth muscle. Arch.Hist.Jap.,41,No.3,239-258. DOI:

Barratt, L ,(1980),Spontaneous activity and related calcium movement of Fish intestinal smooth muscle. ph. D. Thesis, Lancaster Univ.

Barratt, L, and Huddart, H ,(1979). Sponaneous activity and related Ca+2 movement of Fish intestinal smooth muscle, the effect of depolarization, Caffeine and Lanthanum. Gen. pharmac. ,10,21-30. DOI:

Batra, S, (1973).The role of mitochonderia calcium uptake in contraction and relaxation of myometerium Biochem. Bio phy.Acta.,305,428-432. DOI:

Bauer, V, Kadlec ,D, and Seferna, L,(1974).Antinecotinic action of papaverine on intestine, Pharmac. Res. Comm..vol.6, 35-46. DOI:

Bonev, A, Boev, K, and Spassov, G,(1988).Photo-induced removal of Nifedipine Blocked of Ca+2 entry in different phases of gastric plateau action.

Meth. and Find Exp. Pharmacal., 10(2): 111 – 115.

Bueding, E, Butcher, R, W, Hawkins, J, and Sutherland, E, E, (1966).The effect of epinephrine on cyclic 3,5-AMP and hexose. Phosphate in intestinal smooth muscle. Biochem. Biophys. Acta. ,115, 173-178. DOI:

Devine ,C, E, Somlyo, A, V, and Somlyo, A,P,(1973).Sarcoplasmic reticulm and mitochondria as action accumulation sites in smooth muscle-phil. Trans. R. Soc. Lond. ,B. , 265 : 17-23. DOI:

Diamond , J,(1977). Evidence for dissociation between cyclic Nucleotide levels and tension in smooth muscle in : The Biochemistry of smooth muscle. Edited by N. L. Stephens. Pp. 343-360.Univ. part press, Baltimore, London.

Diamond , J, and Marshal, J, M, (1969). Smooth muscle relaxation dissociation between resting membrane potential and resting tension in rat myometrium. J. Pharmac. Exp. Ther. ,168: 13 -20.

Gabella , G, and Raeymaeker , L, (1976). Effect of Collagenase on mechanical activity and fine structure of an intestinal smooth muscle.cell. Tiss.Res. 173, 29 – 44. DOI:

Gaino , R, M, and Trento, M, (1984). Prostacyclin induced contraction of Guinea – Pig ileum ; influence of drugs affecting calcium flux in the smooth muscle. European. J. Pharmc. , 102 , 529 -533. DOI:

Goodford, P, J, (1967). The calcium content of the smooth muscle of Guinea-pig taenia coli. J. Physiol (London) , 192 : 145 -147. DOI:

Hasselbach, W, (1964).Relaxation and the sarcotubular pump. Fendn. Proc. 23 , 909 -912.

Han, dong Han , Geun, K, Y, Kim, &Jin, C, (2007). The effect of papaverine on ion basilar smooth muscle cells. Neurological Research. DOI:

vol. 29. No. 6.pp 544 – 550(7).

Huddart , H, Bayton, E, and Shanklin, J, (1983). Influence of some common methylzanthines on contractile response and calcium mobilization of ileal , Vas deferens and bladder smooth muscle. J. Exp. Biol., 107 : 73 -93. DOI:

Huddart, H, and Hunt , S, (1975) , Visceral muscle – its structure and Function. Blackie, Glasgow.

Huddart , H, Hunt, S, and Oates , K,(1977). Calcium movement during contraction in mollscan smooth muscle and the loci of calcium binding and release. J. Exp. Biol. ,68 : 45- 46. DOI:

Huddart , H, Langton , P, D, and Saad, K, H, M, (1984). Inhabitation by papaverine of calcium movement and tension in the smooth muscle of rate Vas deferens and urinary bladder. J. Physiol., 49 : 183 -194. DOI:

Huddart , H, and Saad, K,H, M, (1977). Qunine and lanthenium effect on contractility and calcium movement of rat ileal smooth muscle. Gen. Pharmac; 8 , 341 – 347. DOI:

Iguchi, M, Nakajima, T, Hisasda, T, Sugimoto, T, and Kurachi, Y,(2006). On the mechanism of papaverine inhibition of the voltage-dependent Ca+2 current in isolated smooth muscle cells from the guinea pig trachea.J. of Ethnophamacology.vol. 105, Issue 3.

Kaneda, T, Shimizu, K, Nakajyo, S, & Urakawa, N, (1998).The difference in the inhibitory mechanisms of papaverine on vascular and smooth muscles.European J. of Pharmc. Vol. 355, 3 , DOI:

Koutsoviti, M, Papadopoulou,T, A, Psarra & Batzias, G,(2008).Milrinone and the Ophylline act as lower esophageal sphincter relaxing agents : a comparative pharmacodynamic study in the rabbit. J. of Veterinary Pharmac. And Therapentics. Vol. 32 Issue 2- page 177-181. DOI:

Kuraishi, Y, Morota, T, Asano, T, & Tani , T, (2006) Glycycoumarin from Glycyyrrhizae radix acts as a potent antispasmodic through inhibition of phsphodiesterase J. of Ethnophamacology.vol. 105, Issue 3. DOI:

Okamyiya, Y, Kishimoto, T, & Komiko, A, (1991). Effect of TC-81, a new dihydropyridine, on K+- induced contraction in rat aorta.

European J. of Pharmc. Vol. 205.

Malmstrom, K, and Carafoli, E, (1977). The interaction of Ca+2 with mitochondria from human mitochondria.Arch. Biochem. Biophys. DOI:

, 657 – 666.

Miyamoto , M, Takayanagi, L, Ohkubo, H, and Takayi , K, (1976). Actionof papaverine on intestinal smooth muscle and it's inhibition of cyclic –AMP and cyclic-GMP phosphodiesterase Jap. J. Pharmac. 26, 114 – 117. DOI:

Nilsson , K, B, Andersson, R,G, Mohme-Iudhom, E, and Lundholm, L,(1978). Some prperties of Ca+2 – binding subfraction isolated from rabbit colon muscle. Acta. Pharmac. Toxical. 42 , 185 -193. DOI:

Reinhadrt , D, Roggenboch, W, Brodde, D,E, and Schumann, H, J, (1977).Influence of papaverine and isoprenaline on contractility and cyclic AMP level of left Guinea –pig artia at different rates of beat. Nauyn – Schmiedebergs Arch. Pharmac. 229 , 2 -15. DOI:

Saad , K, H, M, (1980). Calcium regulation during Excitation – contraction coupling of mammalian smooth muscle.Ph. D. Thesis ,Univ. of Lancaster.

Saad, k, H, M,(2006).The effect of Procaine on the mechanical and enzyme activity of ileum smooth muscle of the rat. In the press.

Saad , K, H, M, Ibrahem, S, S, and Shawkat, S,S,(1988).The effect of quinine on spontaneous rhythmic contraction of rabbit ileal smooth muscle. Iraq, J. Agricu. scien.,6 (1),7-16.

Santi, R, Ferrari, M, and contessa, A, R, (1964).On the mechanism of spasmolytic effect of papaverine and certain derivatives, Biochem. 13 , 153 – 158 DOI:

Shibata, N, Ichioka, S, & Kamiya, A, (2005).Estimating oxygen consumption rates of arteriolar walls under Physiological condition in rat skeletal muscle. Am. J. Physiol Heart Circ. Physiol 289 : H295-H300. DOI:

Shimizu, K, Ichikawa, T, Urakawa, N, & Nakajyo, S, (2000). Inhibitory mechanism of papaverine on the smooth muscle of Guinea pig Urinary bladder. The Japanese J. Of Pharmc. Vol. 83: No. 2. DOI:

Shimizu, K, Yoshihara, E, Takahashi, M, Gotoh, K, Orita, S, Urakawa, N, & Nakajyo, S,(2000). Mechanism of relaxant response to papaverine on the smooth muscle of Non-pregnant rat Uterus.J. Of smooth muscle Research. Vol. 36 No. 3. DOI:

Tomiyama , A, Takayamai, L, and Takagi, K, (1973). Relaxation of intestinal smooth muscle and calcium movement. J. Pharmac. 25, 65 – 68. DOI:

Triner , L, Nahas , G, G, Vulliemoz, Y, Overwec, N, I, A, Verosky, M, Habif , D, V, and Nagi, S, H,(1971). Cyclic-AMP and smooth muscle function. Ann. N. Y. Acad. Sci. 185, 458 – 476. DOI:

Urnno , T, Takayanagi, L, Tokunaga, M, Kubota, K, and Takagi, K,(1974). Action of papaverine aspaminol and bile salts on intracellular cyclic-AMP level Jap. J. Pharmac. 24, 681 – 686. DOI:

Wali F, A, and Greenid, E, (1986). Effect of calcium antagonists on vascular responses of bovine coronary artery to acetylcholine , noradrenaline, and 5-hydroxytryptamine. Jap. J. Physiol. , 36, 807 – 813. DOI:

Weber, A, M, (1966). Energized calcium transport and relaxation factor in Current to pieces in Bioenergetics. L. 203 – 254. DOI:

William, A, J, (1976).The effect of Quinine on calcium transport in rat cardiac muscle. Ph.D. Thesis, Lancaster, Univ.



How to Cite

Saad, K. H. M. . (2012). The effect of papavarine on contractile and enzyme Activity and on Oxygen consumption of ileal smooth muscle of laboratory mice. Al-Mukhtar Journal of Sciences, 27(1), 64–84.



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