The Effect of Nicotiana glauca Leaf Extract on the Liver and Lung of Female Albino Mice: Physiological and Histopathological Studies

Authors

  • Salmin Omar Zoology Department, Faculty of Science, Benghazi University Benghazi Libya
  • Abdalla I. Mohamed Zoology Department, Faculty of Science, Benghazi University Benghazi Libya
  • Fatima Omar Pharmacology and Toxicology Department, Faculty of Pharmacy, Benghazi University Benghazi Libya
  • Soad Alwerfali Zoology Department, Faculty of Science, Benghazi University Benghazi Libya

DOI:

https://doi.org/10.54172/mjsc.v35i2.302

Keywords:

Nicotiana glauca, Female Mice, Physiology, Histopathology

Abstract

  Tobacco Nicotiana glauca is a member of the Solanaceae family, found in tropical and subtropical countries especially South America, Cuba, and Brazil, as well as in North Africa, Egypt, and Libya. N. glauca is known to be a highly toxic plant. The major alkaloids are anabasine and nicotine. This study was aimed to determine the proper effects of a sublethal dose of the plant on female albino mice, as well as certain histological and physiological parameters. The three sublethal concentrations, 0.25, 0.50, and 2.8 mg/kg of the water extract were tested through oral route on female mice for determination of effects such as body weight, liver and lung weight and histology, blood parameters, and blood chemistry. The results showed that reduced body weight was slightly evident in treated females. Percent decrease of liver weight/body weight and increase lung weight/body weight have reported in 2.8 mg/kg treated female mice compared to control mice. Histology of liver and lung revealed an early sign of cell damage and accumulation of cell necrosis in both the liver and lungs. The blood parameters and blood chemistry did not reveal a significant difference between control and N. glauca treated female mice.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

References

Alder, L. S., Seifert M. G., Wink. And Morse, G. E. (2012). Reliance on pollinators predicts defensive chemistry across tobacco species. Ecol. Lett., 15: 1140-1148. DOI: https://doi.org/10.1111/j.1461-0248.2012.01838.x

Benowitz, N. L. and Jacob, P. III. (1997). Individual differences in nicotine kinetics and metabolism in humans [Review]. Natl. Inst. Drug Abuse. Res. Monogr., 173: 48-64. DOI: https://doi.org/10.1037/e495572006-006

Goodman, L. S. and Gilman, A. (1985). The Pharmacological Basis of Therapeutics. 7th Edition, MacMillan Publishing Co., New York.

Gosseline, R. E., Smith, R. H. and Hodge, H. C. (1984). Nicotine In :Clini. Cal. Toxicology of Commerical products Fifth Edition, Williams and wikins, Baltimor, Maryland, Williams andwikins, Baltimor, Maryland, pp. III-311-314.

Gupta, P. K. (2016). Toxic effects of cardiac poisons. in Fundamentals of toxicology. 21.4. DOI: https://doi.org/10.1016/B978-0-12-805426-0.00021-4

Janbaz, K. H. and Gilani, A. H. (1995). Evaluation of the protective potential of Artemisia maritime extraction acetaminophen- and CCl4-induced liver damage. J. Ethnopharmacol., 47: 43-47. DOI: https://doi.org/10.1016/0378-8741(95)01252-9

Long, N., Ren, X., Xiang, Z., Wan, W. and Dong, Y. (2016). Sequencing and characterization of leaf transcriptomes of six diploid Nicotiana species. J. Biol. Res., (Thessalon). 23: 6. DOI: https://doi.org/10.1186/s40709-016-0048-5

Manoguerra, A. S. and Freeman, D. (1982). Acute poisoning from the ingestion of Nicotianaglauca. J. Toxicol. Clin. Toxicol. 19: 861-864. DOI: https://doi.org/10.3109/15563658208992520

Melamed, H. T., Markman, S., Aieli, A., Dist, M., Wink, M. and Tzhaki, I. (2004). Limited ability of Palestine sunbirds Nectarinia osea to cope with pyridine alkaloids in necter of tree tobacco Nicotiana glauca. Func. Ecol., 18: 844-850. DOI: https://doi.org/10.1111/j.0269-8463.2004.00929.x

Mellick, L. B., Makowski, T., Mellick, G. A. and Borger, R. (1999). Neuromuscular blockade after ingestion of tree tobacco (Nicotiana glauca). Ann. Emerg. Med., 34: 101-104. DOI: https://doi.org/10.1016/S0196-0644(99)70280-5

Mitchell, J. W. and Breyer-Brandwijk, E. (1962) Medicinal and poisonous plants of southern and Eastern Africa-2nd ed. and S livingston; 986.

Ntelios, D., Kargakis, M., Topalis, T., Drouzas, A. and Potolidis, E. (2013). Acute respiratory failure due to Nicotiana glauca ingestion. Hippokartia., 17 (2): 183-184.

Panter, K. E., James, L. F. and Gardner, D. R. (1999). Lupines, poison hemlock and Nicotiana ssp.: toxicity and teratologenicity in livestock. J. Nat. Toxins., 8 (1):117-134.

Preece, A. H. (1972). Amanual for Histologic Technicians, 4th ed. (227-231). Little, Brown and company. Boston.

Rotenberg, K. S. (1982). The pharmacokinetics of nicotine. Pharm. Int., 3: 91-93.

Rowell, P. P., Hurst, H. E., Marlowe, C. and Bennett, B. D. (1983). Oral administration of nicotine: Its uptake and distribution after Chronic administration to mice. J. Pharmacol. Methods, 9: 249-261. DOI: https://doi.org/10.1016/0160-5402(83)90064-5

Saneck, R., Gupta, R. C. and Kadel, W. L. (1994). Lethal nicotine intoxication in a group of mules. J. Vet. Diagn. Invest.,6: 503-504. DOI: https://doi.org/10.1177/104063879400600422

Schep, L. J., Slaughter, R. J. and Beasley, D. M. (2009). Nicotinic plant poisoning. Clin. Toxicol. (Phila.), 47: 771-781. DOI: https://doi.org/10.1080/15563650903252186

Schievelbein, H. and Balfour, D. J. K. (1984). Nicotine, reabsorption and fate. In: Nicotine and the smoking Habit, international Encylopedia of pharmacology and Therapeutics, Section 114, Pergamon Press, New, York., pp. 1-12.

Sokal, R. R. and Rodlf, F. F.(1969) Biometry, the principle of statistics in biological research. W. H. freeman and company, san fransisco, USA.

Webb, M. and Dalzell, S. (1997) Nicotiana glauca toxicity. Emergency. Med., 9: 25-28. DOI: https://doi.org/10.1111/j.1442-2026.1997.tb00552.x

Wilson, R., & DeEds, E. (1936). Chronic nicotine toxicity. I. Feeding of nicotine sulfate, tannate, and bentonite. J. Int. Hyg. Toxicol, 18, 553-564.

Wink, M. (2000). Interference of alkaloids with neuroreceptors andionchannels. Bioactive Natural Products, Vol. 11 (ed. Atta-Ur-Rahman), pp. 3–129. Elsevier Science, Amsterdam. DOI: https://doi.org/10.1016/S1572-5995(00)80004-6

Downloads

Published

2020-06-30

How to Cite

Omar, S. ., Mohamed, A. I., Omar, F. ., & Alwerfali, S. (2020). The Effect of Nicotiana glauca Leaf Extract on the Liver and Lung of Female Albino Mice: Physiological and Histopathological Studies. Al-Mukhtar Journal of Sciences, 35(2), 94–102. https://doi.org/10.54172/mjsc.v35i2.302

Issue

Section

Research Articles

Categories

Most read articles by the same author(s)

Obs.: This plugin requires at least one statistics/report plugin to be enabled. If your statistics plugins provide more than one metric then please also select a main metric on the admin's site settings page and/or on the journal manager's settings pages.