Isolation and Identification of Fungi Contaminating Potato Chips Intended for Children’s Consumption and Assessing their Toxins

Authors

  • Hadeel A. AL-Ameri Department of Biology, Faculty of Sciences, Mosul University-Iraq
  • Nadeem A. Ramadan Department of Biology, Faculty of Sciences, Mosul University-Iraq

DOI:

https://doi.org/10.54172/mjsc.v35i4.332

Keywords:

Contaminated Chips, Mycotoxins, Aflatoxins, Zearalenone, Ochratoxin

Abstract

The study aims to investigate the presence of fungi and their toxins in different samples of potato chips imported from different origins. Fifteen chips’ samples were collected from the local markets of Mosul city/Iraq which included various global origins with several flavors of pepper, paprika, hot spices, and cheese. It appears that all potato chips’ samples were contaminated with fungi and mycotoxins. It was evident that Penicillium spp. were the most predominant fungi followed by Aspergillus spp. and Rhodotorella spp. came third, while Geotrichumspp and yeasts came in fourth. Potato chips from the brand Pringles was contaminated with Penicillium spp., and Geotrichum spp. at a percentage of 50, 30% respectively. The Hum Hum brand samples were contaminated with A.terrus and Penicillium spp. at 40, 30% respectively. Dream brand samples were contaminated with A.jamanicum., and Penicillium., at percentages of 30, 60% respectively. Lays1 with tomato ketchup samples were contaminated with four genera: Penicillium spp., Mucor spp., Rhodotorellaspp., and yeast with percentages of 40, 20, 10, and 10% respectively. Lays2 with French cheese variety was contaminated with the same fungi of laystype but with the addition of Aspergilli (A. versicolor and A. niger), which were 60 and 30% respectively. The Patos brand potato chips were contaminated with two Aspergilli (A.astus and A.jamanicum), at 30 and 40% respectively. Zearalenone was found to be the highest contaminant (13.81ppm) of mycotoxins followed by aflatoxins (0.26ppm). Ochratoxin was the least contaminant (0.16ppm) in the analyzed potato chips. It can be concluded that all tested potato chips’ samples showed the presence of fungi and mycotoxins. However, all mycotoxins (aflatoxin, ochratoxin, and zearalenone) in the food commodities were within the permissible limits intended for human consumption.

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Author Biography

Nadeem A. Ramadan, Department of Biology, Faculty of Sciences, Mosul University-Iraq

 

 

References

Amri, E., & Lenoi, S. o. (2016). Aflatoxin and fumonisin contamination of sun-dried sweet potato (Ipomoea batatas L.) Chips in Kahama District, Tanzania.

Azizi, I., Ghadi, H., & Azarmi, M. (2012). Determination of aflatoxin B1 levels of the feedstuffs in traditional and semi-industrial cattle farms in Amol, northern Iran. Asian Journal of Animal and Veterinary Advances, 7(6), 528-534. DOI: https://doi.org/10.3923/ajava.2012.528.534

Bahrami-Samani, O., Rahimi, E., & Nili-Ahmadabadi, A. (2017). Assessment of zearalenone contamination in processed cereal-based foods in Shahrekord, Iran. Toxin Reviews, 36(3), 257-260. DOI: https://doi.org/10.1080/15569543.2016.1178148

Bankole, S., & Adebanjo, A. (2003). Mycotoxins in food in West Africa: current situation and possibilities of controlling it. African journal of Biotechnology, 2(9), 254-263. DOI: https://doi.org/10.5897/AJB2003.000-1053

Bullerman, L. B., & Bianchini, A. (2007). Stability of mycotoxins during food processing. International journal of food microbiology, 119(1-2), 140-146. DOI: https://doi.org/10.1016/j.ijfoodmicro.2007.07.035

Christensen, C. M., Mirocha, C. J., & Meronuck, R. A. (1977). Molds, mycotoxins, and mycotoxicoses [produced by Fusarium, Aspergillus, contaminated feeds]. Cereal Foods World.

Croft, W. A., Jarvis, B. B., & Yatawara, C. (1986). Airborne outbreak of trichothecene toxicosis. Atmospheric Environment (1967), 20(3), 549-552. DOI: https://doi.org/10.1016/0004-6981(86)90096-X

de Hoog, G. S., & Guarro, J. (1995). Atlas of clinical fungi (Spain, Ed.). Universal RoviraIvirgili, Reus.

Do, J. H., & Choi, D.-K. (2007). Aflatoxins: detection, toxicity, and biosynthesis. Biotechnology and Bioprocess Engineering, 12(6), 585-593. DOI: https://doi.org/10.1007/BF02931073

Gnonlonfin, G., Adjovi, C., Katerere, D., Shephard, G., Sanni, A., & Brimer, L. (2012). Mycoflora and absence of aflatoxin contamination of commercialized cassava chips in Benin, West Africa. Food Control, 23(2), 333-337. DOI: https://doi.org/10.1016/j.foodcont.2011.07.026

Jonathan, S., Abdul-Lateef, M., Olawuyi, O., & Oyelakin, A. (2012). Studies on bio-deterioration, aflatoxin contamination and food values of fermented, dried and stored Ipomoea batatas chips. Nature and Science, 10(11), 123-128.

Krnjaja, V., Lević, J., Stanković, S. Ž., Petrović, T. S., Tomić, Z., Mandić, V., & Bijelić, Z. (2013). Moulds and mycotoxins in stored maize grains. Biotechnology in Animal Husbandry, 29(3), 527-536. DOI: https://doi.org/10.2298/BAH1303527K

Manjula, K., Hell, K., Fandohan, P., Abass, A., & Bandyopadhyay, R. (2016). Aflatoxin and fumonisin contamination of cassava products and maize grain from markets in Tanzania and republic of the Congo. Journal of Applied & Environmental Microbiology, 4(34), 55-62.

Martins, M. L., Martins, H. M., & Bernardo, F. (2001). Aflatoxins in spices marketed in Portugal. Food Additives & Contaminants, 18(4), 315-319. DOI: https://doi.org/10.1080/02652030120041

Milani, C., Hevia, A., Foroni, E., Duranti, S., Turroni, F., Lugli, G. A., Sanchez, B., Martin, R., Gueimonde, M., & van Sinderen, D. (2013). Assessing the fecal microbiota: an optimized ion torrent 16S rRNA gene-based analysis protocol. PloS one, 8(7), e68739. DOI: https://doi.org/10.1371/journal.pone.0068739

Mmasa, J., Msuya, E., & Mlambiti, M. (2012). Socio-economic factors affecting consumption of sweet potato Products: An empirical approach.

Nelson, P. E., Toussoun, T. A., & Marasas, W. (1983). Fusarium species: an illustrated manual for identification.

Park, D. L., & Stoloff, L. (1989). Aflatoxin control—How a regulatory agency managed risk from an unavoidable natural toxicant in food and feed. Regulatory Toxicology and Pharmacology, 9(2), 109-130. DOI: https://doi.org/10.1016/0273-2300(89)90029-9

Pitt, J. I., & Hocking, A. D. (2007). Fungi and food spoilage (Vol. 519). Springer.

Savino, M., Limosani, P., & Garcia-Moruno, E. (2007). Reduction of ochratoxin A contamination in red wines by oak wood fragments. American journal of enology and viticulture, 58(1), 97-101.

Tairo, F., Mneney, E., & Kullaya, A. (2008). Morphological and agronomical characterization of sweet potato [Ipomoea batatas (L.) Lam.] germplasm collection from Tanzania. African Journal of Plant Science, 2(8), 077-085.

Thieu, N. Q., Ogle, B., & Pettersson, H. (2008). Screening of Aflatoxins and Zearalenone in feedstuffs and complete feeds for pigs in Southern Vietnam. Tropical Animal Health and Production, 40(1), 77-83. DOI: https://doi.org/10.1007/s11250-007-9056-7

Wyllie, T. D., & Morehouse, L. G. (1978). Mycotoxic fungi, mycotoxins, mycotoxicoses.

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Published

2020-12-31

How to Cite

AL-Ameri, H. A. ., & Ramadan, N. A. . (2020). Isolation and Identification of Fungi Contaminating Potato Chips Intended for Children’s Consumption and Assessing their Toxins. Al-Mukhtar Journal of Sciences, 35(4), 273–283. https://doi.org/10.54172/mjsc.v35i4.332

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