Comparative Study Using Simple Methods in Preservation of Some Microorganisms
DOI:
https://doi.org/10.54172/mjsc.v7i1.408Keywords:
Preservation Methods,, Microorganisms, FreezingAbstract
Freezing (–20°C) in corn oil, agar disc, gelatin disc, filter paper and wheat seeds were compared for preservation of single cultures of Pseudomonas spp., Escherichia coli, Lactobacillus bulgaricus, Streptococcus thermophilus and Saccharomyces cerevisiae up to 50 weeks.
All the cultures under investigation were found to preserve successfully with these methods of preservation except Lactobacillus bulgaricus which failed to maintain their viability with agar disc, gelatin disc and filter paper.
According to this study, it is suggested that using filter paper is of beneficial value in preserving Saccharomyces cerevisiae.
With Pseudomonas spp., Escherichia coli, and Streptococcus thermophilus
Downloads
References
AL–Dabbagh, W. Y. 1994. Comparative study on the preservation of L. bulgaricus and S. thermophilus Cultures. Mesopotamia J. Agric. 26, 11–5.
Davis, J. G., 1975. The microbiology of Yoghurt. In: Carr, J. G. Cutting, C. V. and Whiting, G. C. (eds.) Lactic acid bacteria in beverages and Food. Academic Press, London, PP. 257.
Holt, J. G., Krieg. N. R., Sneath, P. H., Staley, J. T. and Williams, S. T. 1994. Bergey’s Manual of Determinative Bacteriology. Ninth ed Williams and Wilkins, Baltimore, Maryland, U.S.A.
Scott, W. J. 1958. Method for the dried storage of microorganisms. Brit. Pat No. 799, 44.
Snell, J. J. S. 1984. General introduction to maintenance methods. In: Kirsop, B. E. and Snell, J. J. S. (eds). Maintenance of Microorganisms. A manual of laboratory methods. Academic Press, London. PP. 11–21.
Tsvetkov. T. and I. Shishkova. 1982. Studies on the effects of low temperatures on. Lactic acid bacteria. Cryobiology, 19, 211–214. DOI: https://doi.org/10.1016/0011-2240(82)90143-2
WFCC “World Federation Culture Collection” 1988. Workshop, Preservation of microorganisms. 27th – 29th Oct., Univ. of Maryland U.S.A.
Downloads
Published
How to Cite
License
Copyright (c) 2021 Wail Al–Dabbagh , Aala R. Ali
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Copyright of the articles Published by Almukhtar Journal of Science (MJSc) is retained by the author(s), who grant MJSc a license to publish the article. Authors also grant any third party the right to use the article freely as long as its integrity is maintained and its original authors and cite MJSc as original publisher. Also they accept the article remains published by MJSc website (except in occasion of a retraction of the article).
