دراسة لمرض موازيك الفلفل المتسبب عن الفيروس Potato Virus Y بمنطقة المرج – الجبل الأخضر

Yunis Ali  Taib; Omar Mussa  Alsanousi; Fathi S.  El-Musmary

doi:10.54172/mjsc.v8i1.422

Authors

Yunis Ali Taib Department of Plant Protection, Faculty of Agriculture, Omar Al-Mukhtar University, PO Box 919 Al-Bayda - Libya.
Omar Mussa Alsanousi Department of Plant Protection, Faculty of Agriculture, Omar Al-Mukhtar University, PO Box 919 Al-Bayda - Libya.
Fathi S. El-Musmary Department of Horticulture, Faculty of Agriculture, Omar Al-Mukhtar University, Al-Bayda, Libya.

DOI:

https://doi.org/10.54172/mjsc.v8i1.422

Keywords:

Mosaic Disease, Pepper Plant , EL-Marj, Libya

Abstract

Asurvey conducted during spring 2000 of the diseases affecting pepper cultivars grown in EL-Marj county of Green mountain district, revealed a wide spread of viral diseases. Its symptoms was manifested as dwarfing of infected plants, mosaic, chlorosis and vein banding of the leaves. The virus was readly transmitted to healthy plants by mechanical inoculation and Green peach aphids in non-pesistent manner. Preliminary studies on physical properties, host range, differential hosts and cytopathological alteration indicates that the virus has a thermal inactivation point (TIP) of 60C°, a dilution end point (DEP) of 5× 10^-3 – 5× 10^-4 , longevity in vitro (LIV) of 11 days, infecting only solanaecious plants including, Datura metel several pepper cultivars and some tobacco species among the 36 different plant species and cultivars assyed and has a flexuous virus particles and pin-wheel inclusion bodies in thin sections of infected tissues, a characteristic feature of poty virus group. Serological studies using indirect ELISA technique confirmed the association of potato virus Y (PVY) with the disease syndrome. Screening of pepper varities for resistance and susceptibility showed that none of commonly grown cultivars was immune to infection.

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References

السنوسي ، عمر موسى ، محمد عبد المجيد شقرون وجبر عبد الله خليل (1991) : عزل وتعريف فيروس البطاطا Y من نباتات الفلفل في ليبيا ، مجلة وقاية النبات العربية ، 9 (1) : 47-51 .

أبو غنية ، عبد النبي (1986) : أمراض المحاصيل البستانية ، جامعة الفاتح ، الجماهيرية الليبية .

حسن ، أحمد عبد المنعم (1984) : الخضر الثمرية ، الدار العربية للنشر والتوزيع .

مطلوب ، عدنان ناصر ، عز الدين سلطان محمد وكريم صالح عبدول (1989) : إنتاج الخضراوات ، الجزء الثاني – مديرية دار الكتب للطباعة والنشر - جامعة الموصل .

يونس ، نضال ذنون (2000) : دراسات عن بعض الفيروسات المسببة لأعراض الموزايك على محصول الفلفل في محافظة نينوى بالعراق ، رسالة ماجستير ، كلية الزراعة والغابات ، جامعة الموصل .

Batarseh, S. F. (1985): Plant veruses affecting peppers in Jordan. M. Sc thesis, Faculty of Agriculture. Univ. of Jordan.

Bisheya, F. A. (1980): Identification and cytological studies of watermelon mosaic virus affecting squash. Cucurbita pepo L. In Tripoli area, M. Sc thesis Faculty of Agriculture. University of El-Fateh, Libya.

Black, L. L. Green, S. K. Hartman, G. L. and Poulos, J. M. ( 1991) Pepper diseases. Afield guide. Asian vegetable Research and Development center, (ABRDC) publ. No. 91. 347.

Boiteux, L. S. Cupertino, F. P. and Silva, C. (1996) Resistace to potato virus Y (patho type 1-2) in Capsicum annuum and C. chinense is controlled by two independent major genes, Euphytica. 87:53-58. DOI: https://doi.org/10.1007/BF00022964

Bos, L. (1970) Bean Yellow mosaic virus CMIAAB Description of plant viruses No. 40 .

Bos, L. (1983) Introduction to plant virology. Center fr Agricultural publishing and documentation. Wageningen 160 pp.

Brunt, A. A. and R. H. kenten. (1971) Pepper veinal mottel virus a new member of the potato virus Y. group from peppers (Capsicum annuum L. and C. frutescens L. in Ghana. Ann. Appl. Biol. 69:235-243. DOI: https://doi.org/10.1111/j.1744-7348.1971.tb04676.x

Brunt, A. A. and R. H. kenten. (1972) Pepper veinal mottel virus CMIAAB Description of plant viruses, No. 104.

Budnik, K. ; Laing, M. D. ; Graca, Jv. Da, and Da-Graca, J. B. (1996) Reduction of yield losses in pepper crops caused by potato virus Y in Kwazulu-Natal. South Africa, using plastic mulch and yellow sticky traps. Phytoparasitica 24:119-124. DOI: https://doi.org/10.1007/BF02981406

Collar, T. L. ; Arillaa, C. ; Duque, M. and Fereres, A. (1997) Behavioral respons and virus vector ability of Myzus persicae (Homoptera:Aphididae) probing on pepper plants treated with aphicides, J. of Economic Entomology. 90;1628-1634. DOI: https://doi.org/10.1093/jee/90.6.1628

Deboks. J. A. and Huttinga, H. (1981) potato virus Y. CMIABB Description of plant Viruses No.242.

Delgado-Sanchez, S. & Grogan, R. G. (1970) potato Virus Y. CMIABB Description of plant Viruses No. 37.

Edwardson, J. R. (1966) Electron microscopy of cytoplasmic inclusions in cells infected with rod-shaped viruses American Jornal Botany 53:359-364. DOI: https://doi.org/10.1002/j.1537-2197.1966.tb07347.x

Elsanusi, O. M. (1988) identification of two viruses causing mosaic disease in pepper plants in Tripoli and Gharabuli areas. Msc. thesis, Faculty of Agriculture, University of Elfateh, Libya.

Fereres, A. ; Perez. P. ; Gemens, C. and ponz, F. (1993) Transmission of Spanish pepper and potato-PVY isolates by aphid (Homoptera:Aphididae) vectors: epidemiological implications. Environmental. Entomology 22: 1260-1265. DOI: https://doi.org/10.1093/ee/22.6.1260

Fegla, G. I. El-Samara, I. A. Noaman, K. A. and Younes, H. A. (1997) Host rang, transmission and serology of an isolate of tomato yellow leaf curl virus from tomato of plastic houses in northern of agricultural sciences Faculty of agric. Assiut. Univ, Assiut, No. 1:13-14.

Feldman, J. M. and O. Gracia, (1977) Pepper severe mosaic virus Anew poty virus from pepper in Argentina phytopathology 87:146-160. DOI: https://doi.org/10.1111/j.1439-0434.1977.tb02851.x

Garanta, C. and pulloix, A. (1995) Both common and specific genetic factors are involved in polygenic resistance of pepper to several. Poty viruses. Theor. App L. Genetic 92:15-20. DOI: https://doi.org/10.1007/BF00222946

Gibbs, A. J. and B. Harrison. (1976) plant virology. The principles, Edward Arnold, London. 292 pp.

Gibbs, A. J. and Harrison, B. D. (1970) Cucumber mosaic virus CMIAAB Desctiption of plant viruses No. 1.

Gracia, O. and Feldman, J. M. (1979) Virus disease of pepper in Mendoza and Sanjuan provinces. India, 3, 349-354, 80-85. Rev Plant pathology 59, 5512. (Abstr).

Lloyd. Thomas, O. S. (1982) Hot pepper mosaic an important disease in the West Indies. Tropical Pest Management. 28:88-89. Rev plant pathology. 61, 5419 (1982) (Abstr). DOI: https://doi.org/10.1080/09670878209370678

Lowery, D. T. Eastwell, k. C. and Smirle, M. J. (1997) Neem seed oil inhibits aphid transmission of potato virus Y to pepper. Annuls of Applied-Biology. 130:217-225. DOI: https://doi.org/10.1111/j.1744-7348.1997.tb06827.x

Makkouk, K. M. and Gumpf, D. J. (1976) Characterization of potato virus Y strains isolated from pepper phytopathology. 66:576-581. DOI: https://doi.org/10.1094/Phyto-66-576

Makkouk, K. M. and Gumpf, D. J. (1974) Further identification of naturally occurring virus diseases of pepper in California. Plant diseases. 58:1002-1006.

Marco, S. and Cohn, S. (1979) Rapid detection and titer evalution of viruses in pepper by Enzym-Linked immunosorbent assay. Phytopathology. 69:1259-1262. DOI: https://doi.org/10.1094/Phyto-69-1259

Meek, G. A. (1976) Practical electron microscopy for biologists. 2 n Edition Awiley-interscience. Publication England. 528pp.

Nelson, M. R. and R. F. wheeler. 91972) A. new virus disease of pepper in Arizona. Plant disease. 56:731-735.

Nelson, M. R. and R. F. Wheeler, (1978) Biological and serological characterizations and separation of potyviruses infecting peppers. Phytopathology. 68:979-984. DOI: https://doi.org/10.1094/Phyto-68-979

Noordam, D. (1973) Identification of plant viruses, Methods and Experiments center for Agricultural publishing and documention. Wageningen. Nether land. 207pp.

Purciful, D. E. T. A. Zitter and E. Hiebert. (1975) Morphology, host rang, and serological relationship of pepper mottel virus. Phytopathology. 65:559-562. DOI: https://doi.org/10.1094/Phyto-65-559

Purciful, D. E. and j. R. Edwardson, (1967). Watermelon mosaic virus:tubular inclusion in pumpkin leaves and aggregates in leaf extracts. Virology. 32:393-401. DOI: https://doi.org/10.1016/0042-6822(67)90289-9

Smith, P. G. (1970) Tobacco etch strains on peppers. Plant disease. 54 (9):786-787.

Smith, K. M. (1972) A text book of plant virus Diseases, Longman Group Ltd. London. 684pp.

Weintraub, M. and Ragetli, H. W. J. (1968) Fine structure of inclusions and organelles in vicia faba infected with bean yellow mosaic virus. Journal of Cell Biology. 38:316-329. DOI: https://doi.org/10.1083/jcb.38.2.316

Younes, H. A. H. (1995) Studies on certain virus diseases affecting some vegetable crops under green houses condition ph. D. Thesis fac. Of Agri, Alex. Uni. Egypt.

Zipora, W. and G. M. Milbrath. (1976). The isolation of tobacco etch virus from bell peppers and weeds in Southern Illinois plant Disease. 60:469-471.

Zitter, T. A. (1972) Naturally occurring pepper virus strains in south Florida. Plant Disease. 56:586-590.

Zobel, R. E. and M. Beer. (1965). The use of heavy metal salt as electron stains. International Review of Cytology 18:363-400. DOI: https://doi.org/10.1016/S0074-7696(08)60558-6