التباين في التعبير الجيني للبروتين الكلي لجذور نبات البصل Allium cepa تحت تأثير تراكيز متدرجة من مبيد الـ Dursban.

Ali Al-Saadi; I. O. El-Awami I. O. El-Awami; N. S.EL-Hadad N. S.EL-Hadad

doi:10.54172/mjsc.v11i1.432

Authors

Ali Al-Saadi Department of Biology. College of Science. Omar Al-Mukhtar University. El-Bieda / Libya
I. O. El-Awami I. O. El-Awami Department of Plant Protection, Faculty of Agriculture, Omar Al-Mukhtar University, Al-Bayda - Libya
N. S.EL-Hadad N. S.EL-Hadad Department of Biology. College of Science. Omar Al-Mukhtar University. El-Bieda / Libya

DOI:

https://doi.org/10.54172/mjsc.v11i1.432

Abstract

The total protein electrophoresis technique is one of the powerful methods of Molecular biology studies to explain the variation of gene expression. This method was used to estimate protein profile under critical conditions. Total protein was extracted from Allium cepa root tips during 2 , 6 and 24 hrs after treated with different concentrations of Dursban (0.03 , 0.04 , 0.06 , 0.08 and 0.12 mg/ml). Results of SDS-PAGE of extracted protein showed that the number of protein bands was proportional with first three concentrations (0.03 , 0.04 and 0.06 mg/ml), where are the expression of additional bands was stopped with the highest concentrations (0.08 and 0.12 mg/ml) of Dursban.This study also aimed to determine the optimal inhibitory effect to volatile and fixed oils of two medical plant species (Origanium majorana and Salvia officinalis) on this pesticide. To achieve this target, the root tips were treated by 1.25 ml/ml of each of these oils before, after and with the treatment of pesticide. The results revealed that the inhibiting effect was associated with the addition of Origanium oils with pesticide in the same time, and with the addition of Salvia oils before treatment with pesticide.

Downloads

Download data is not yet available.

References

Aspelin, A. L. (1994). Pesticides industry sales and usage: 1992 and 1993 market estimates. Washington, D.C. U.S. EPA. Office of prevention, pesticides and toxic substances. Office of pesticide programs. Biological and Economic analysis Division. (June).

Amer, S. H. and F. A. E. Aly, (1992). Cytogenetic effects of pesticides. IV. Cytogenetic effects of the insecticides Gardona and Dursban. Mut. Res. 279: 165-170. DOI: https://doi.org/10.1016/0165-1218(92)90063-6

Abraham, S. (1997). Studies on cytological change induced Murate of Potsh in Allium cepa. Cytologia, 62 : 291-294. DOI: https://doi.org/10.1508/cytologia.62.291

Aliaga – Morell, J. R.; F. A. Culians Macia; G. Clemente Marin and primo, Y. (1987). Differentiation of Rice varieties by Electrophoresis of Embryo protein. Theor. Applied Genetic, 74: 224-232. DOI: https://doi.org/10.1007/BF00289973

Badr, A. (1995). Electrophoretic studies of seed proteins in Relation to Chromosomal Criteria and Relationships of some Taxa of Trifolium. Toxon, 44: 183-191. DOI: https://doi.org/10.2307/1222441

Blazques, M. A.; A. Bono and M. C. Zafra-Polo, (1990). Essental oil from Thymus borgiae, a new Ieriane species of the Hyphodromi section. Jurnal of chromatography, 518: 230-233. DOI: https://doi.org/10.1016/S0021-9673(01)93179-6

Bracco , U., J. Loliger and J.L. Viret, (1981). Production and use natural antioxidants. JAOCS. 58: 686-690. DOI: https://doi.org/10.1007/BF02899449

Chambers, J. E. and R. L. Carr, (1993). Inhibition patterns of brain acetylcholinesterase and hepatic and plasma alisterases following exposures to three phosphorothionate insecticides chlorpyrifos and Fenitrothion by perfused rat liver. Toxicol. 68: 1-9. DOI: https://doi.org/10.1093/toxsci/21.1.111

Collade, C.; R. G. Caballero; R. Casado and C. Aragoncillo, (1988). Different types of major storage seed proteins in Fagaceae species. J. Exp. Bot. 39: 1751-1758. DOI: https://doi.org/10.1093/jxb/39.12.1751

Cooke, R. J. (1989). The use of Electrophoresis for the Distinctness testing of varieties of Autogamouse species. Plant varieties seeds 2: 3-13.

Cuvelier, M. E.; C. Berset and H. Richard. (1994). Antioxidant constituent in sage (salvia officinalis). J. Agric. Food chem. 42: 662-669. DOI: https://doi.org/10.1021/jf00039a012

Dann, E. K. ; P. Mevwly ; J. P. Metraaux and B. J. Deverall. (1996). The effect of pathogen inoculation or chemical treatment of activities of chitinase and b-1,3-glucanase and accumulation of salicylic acid in leaves of green bean. Physiological and Molecular Plant Pathology, 49: 307-319. DOI: https://doi.org/10.1006/pmpp.1996.0056

Deans, S. G.; K. P. Svoboda, (1990). The antimicrobial properties of origanium majorana volatile oil. Flavour and Fragrance Journal. 5 (3): 187-190. DOI: https://doi.org/10.1002/ffj.2730050311

Deflora, S. and C. Ramel, (1988). Mechanisms of Mutagenesis and carcinogenesis classification and over view. Mutat. Res., 202: 285-306. DOI: https://doi.org/10.1016/0027-5107(88)90193-5

Economou,K.D.,V.Oreopoulou and C.D.Thomopoulos,(1991).Antioxidant activity of some plant extracts of the family labiatae. JAOCS .68 (2) : 109-112. DOI: https://doi.org/10.1007/BF02662329

Gilland, T. J. (1989). Electrophoresis of sexually and vegetatively propagated Cultivars of Allogamous species. Plant varieties seeds 2: 15-25.

Gardiner, S. E. and M. B. Forde, (1992). Indentification of Cultivars of Grasses and Forage Legumes by SDS – page of seed protein : 43-61 in seed Analysis (H. F. linkens and J. F. Jackson, eds.) Springer Verlag, Berlin, New York. DOI: https://doi.org/10.1007/978-3-662-01639-8_3

Gardiner, S. E. and M. B. Forde, (1998). Indentification of Cultivars and species of Forage Legumes by sodium Dodecylsulphate polyacrylamide Gel Electrophoresis of seed proteins. Plant varieties seeds. 1: 13-26.

Kada, T.; T. Inoue and M. Namiki, (1982). Environmental desmutagenes and antimutagenes. In : Klekowski, E. J. (Ed.). Environmental Mutagenesis and plant biology. Praeger, New York; 137-151.

Kada, T.; T. Inoue; T. Ohta and Y. Shirasu, (1985). Antimutagens and their modes of action. In : Shankel, D. M., Hartman, P. E., Kada, T. and Hollaende, A. (Eds.). Antimutagenesis and anticarcinogenesis mechanisms. Basic Life Sciences, plenum, New York, Vol. 39: 181-196. DOI: https://doi.org/10.1007/978-1-4684-5182-5_15

Kaur, P. and I. S. Grover, (1985). Cytological effects of some organo phosphorus pesticides 1. Mitotic effects. Cytologia, 50: 187-197. DOI: https://doi.org/10.1508/cytologia.50.187

Kaur, P. and I. S. Grover, (1985). Cytological effects of some organo phosphorus pesticides 11. Mitotic effects. Cytologia, 50: 199-211. DOI: https://doi.org/10.1508/cytologia.50.199

Kim, Y. J. and B. K. Hwang, (1994). Differential Accumalation of b-1, 3-glucanase and chitinase isoforme in papper stems infected by compatible isolates of phytophthora capsici. Physiological and Molecular plant pathology, 45: 195-209. DOI: https://doi.org/10.1016/S0885-5765(05)80077-3

Ladizinsky, G. and T. Hymowitz, (1979). Seed protein Electrophoresis in tanmic and Evolutionary studies – Review – Theor. Applied Genetics, 54: 145-151. DOI: https://doi.org/10.1007/BF00263044

Laemmli, V. (1970). Clearage of structural proteins during the Assembly of the head of Bacteriophage T4. Nature, 227: 680-685. DOI: https://doi.org/10.1038/227680a0

Lawrence, C. B. ; M. H. A. Joosten and S. Tuzun, (1996). Differential induction of pathogenesis-related proteins in tomato by Alternaria solani and the association of a basic chitinase isozyme with resistance. Physiological and Molecular Plant Pathology, 48: 361-377. DOI: https://doi.org/10.1006/pmpp.1996.0029

May, B. ; S. Kohler and B. Schneider, (2000). Efficacy and tolerability of a fixed combination of peppermint oil and caraway oil in patients suffering from functional dyspepsia Aliment pharmacol. Ther. 17(7) : 975-6.

Meige, M. N. (1989). Protein type and Distribution : 291-315 in Nucleic acids and proteins in plants (C. Boulter and B. Parthier, eds) Springer, Berlin.

Patnaik, K. K. and N. K. Tripathy, (1992). Farm-grade chlorpyrifos is genotoxic in somatic and germ – line cells of Drosophila. Mut. Res. 279: 15-20. DOI: https://doi.org/10.1016/0165-1218(92)90261-W

Ramel, C.; V. K. Alekperov; B. N. Ames; T. Kada and L. W. Wattenberg, (1986). Inhibitor of mutagenesis and their relevance to carcinogenesis. Report by ICPEMC expert group on antimutagenes and desmutagenes. ICPEMC publ. No. 12. Mutat. Res., 168: 47-65. DOI: https://doi.org/10.1016/0165-1110(86)90021-7

Rao, B. V.; B. G. S. Rao and C. Sharma, (1988). Cytological effects of herbicides and insecticides on Allium cepa root meristems. Cytologia, 53: 255-261. DOI: https://doi.org/10.1508/cytologia.53.255

Schirone, B.; G. Piovesan; R. Bellarosa and C. Pelosi. (1991). A Taxonomic analysis of seed protein in pinus spp. (Pinaceae). Plant Systematic Evolution, 178: 43-53. DOI: https://doi.org/10.1007/BF00937981

Sheidai, M.; A. Hanita ; A. Hamta ; A. Jaffari and M. R. Norri-Daloii, (1999). Morphometric and seed protein studies of Trifolium species and cultivars in Iran. Plant Genetic Resources News Letters, (120): 52-54.

Sobti, R. C.; A. Krishan and C. D. Pfaffenberger, (1982). Cytokinetic and cytogenetic effects of some agricultural chemicals on human lymphoid cells in vitro : organophosphates. Mut. Res. 102: 89-102. DOI: https://doi.org/10.1016/0165-1218(82)90149-5

Vekiari, S. A.; V. Orcopoulo ; C. Tzia and C. D. Thomopoulos, (1993). Oregano flavonoids as lipid antioxidants. JAOCS. 70(5): 483-487. DOI: https://doi.org/10.1007/BF02542580

Wu, J. W.; M. H. Lee ; C. T. Ho and S. S. Chang, (1982). Elucidation of the chemical structures of natural antioxidants from rosemary. JAOCS. 59: 339. DOI: https://doi.org/10.1007/BF02541016

Yadava,R.N. and V.K.Saini, (1991). Antimacrobial efficacy of essential oils of Majorana hortensis moench and Anisomels indica (Linn.)Kuntze. Indian perfumer , 35 (1) : 58-60.