Evaluation of Efficacy of Collagen-Calcium Alginate Protectants on Chronic Wound Healing in Horses


  • Khaled. M. A. Hussin Department of Clinical Veterinary Medical Sciences, Faculty of Veterinary Medicine, Omar Al- Mukhtar University, Al-Bayda, Liby
  • Asma Saleh W Department of Botany, Faculty of Science, Toubrk University, Toubrk, Libya
  • Rehab Hamad Department of Clinical Veterinary Medical Sciences, Faculty of Veterinary Medicine, Omar Al- Mukhtar University, Al-Bayda, Liby
  • Marwan Saleh Agricultural Research Center, Al-Bayda, Libya




Biomaterial, Collagen-Calcium Alginate, Wound Healing, Chronic Wound


This study aimed to evaluate the efficacy of collagen-calcium alginate as an accelerator of wound healing in a clinical case of ten horses attended at the University Veterinary Hospital (UVH) at Omar Al-Mukhtar University, having non-healing chronic wounds. The pattern of wound healing was evaluated both clinically and by planimetry. All cases showed healing progress after the application of the collagen-calcium alginate film. The study results suggested that collagen-calcium alginate film can be a better wound healing biomaterial in horses. It can be used as a less expensive skin substitute in order to stimulate and promote wound healing in animals especially in the case of chronic non-healing wounds.


Download data is not yet available.


Arul Jothi, N., Thilagar, S., Khaled, M., Thevi, S. G., Lau, S., & Sastry, T. (2006). Clinical evaluation of keratin-gelatin composite film for wound healing in cats. Indian Journal of Veterinary Surgery, 27(2), 108-110.

Ashraf, A.-h. H., & Khaled, M. A. (2008). Diagnosis of joint affection in camels by studies on synovial fluid which give the degree of affections & response to treatment. Al-Mukhtar Journa of Sciences, 20(1), 58-72.

Ballard, K., & Baxter, H. (2000). Developments in wound care for difficult to manage wounds. British Journal of Nursing, 9(7), 405-412. DOI: https://doi.org/10.12968/bjon.2000.9.7.6319

Blaine, G. (1947). Experimental observations on absorbable alginate products in surgery: gel, film, gauze and foam. Annals of surgery, 125(1), 102-114. DOI: https://doi.org/10.1097/00000658-194701000-00011

Bohling, M. W., Henderson, R. A., Swaim, S. F., Kincaid, S. A., & Wright, J. C. (2004). Cutaneous wound healing in the cat: a macroscopic description and comparison with cutaneous wound healing in the dog. Veterinary Surgery, 33(6), 579-587. DOI: https://doi.org/10.1111/j.1532-950X.2004.04081.x

Burrow, T., Welch, M., & Cusick, G. (1983). The development and use of alginate fibres in nonwovens for medical end-uses. Nonwoven Conference Papers, UMIST: 49-68.,

Gopinath, D., Ahmed, M. R., Gomathi, K., Chitra, K., Sehgal, P., & Jayakumar, R. (2004). Dermal wound healing processes with curcumin incorporated collagen films. Biomaterials, 25(10), 1911-1917. DOI: https://doi.org/10.1016/S0142-9612(03)00625-2

Hosgood, G. (2003). Wound repair and specific tissue response to injury,Textbook of small animal surgery. 3rd ed Philadelphia: WB Saunders (Vol., 327-329).).

Khaled, M. A., & Ashraf, A. (2008). Unusual foreign bodies cause obstruction of the esophagus in dogs. Al-Mukhtar Journa of Sciences, 19(1), 51-61.

Khaled, M. A., Asma, S., Almahdi, A., & Kalthum, H. (2016). Clinical Evaluation of Collagen-Calcium Alginate Film along with Therapeutic Ultrasound Massage for Wound Healing in Cats. Acta Biologica Malaysiana, 5(2&3), 49-52.

Khaled, M. A., Asma, S., Nawara, M., A, k., & Marwan, S. W. (2018). Effects of Different Types of Honey on Wound Healing in rats. Al-Mukhtar Journal of Sciences 33(3), 204-213.

Khaled, M. A., Jalila, A., Kalthum, H., Noordin, M., & Saleh, W. A. (2014). Collagen-Calcium Alginate Film Dressing with Therapeutic Ultrasound to Treat Open Wound in Rats. Research Journal of Biological Sciences, 9(2), 57-61.

Kumar, M. S., Sripriya, R., Raghavan, H. V., & Sehgal, P. K. (2006). Wound healing potential of Cassia fistula on infected albino rat model. Journal of Surgical Research, 131(2), 283-289. DOI: https://doi.org/10.1016/j.jss.2005.08.025

Mali, S., Grossmann, M. V. E., García, M. A., Martino, M. N., & Zaritzky, N. E. (2006). Effects of controlled storage on thermal, mechanical and barrier properties of plasticized films from different starch sources. Journal of food engineering, 75(4), 453-460. DOI: https://doi.org/10.1016/j.jfoodeng.2005.04.031

Parker, L. (2000). Applying the principles of infection control to wound care. British Journal of Nursing, 9(7), 394-404. DOI: https://doi.org/10.12968/bjon.2000.9.7.6318

Pope, E. R. (1993). Skin healing .In: Disease mechanisms in small animal surgery. 2nd ED Philadelphia, London, 152-155.

Rafi, M. E. A., Ashraf, A.-h. H., & Khaled, M. A. (2008). Foreign bodies in the rumen of the sheep and goats. Al-Mukhtar Journa of Sciences, 19(1), 38-50.

Rangaraj, A., Harding, K., & Leaper, D. (2011). Role of collagen in wound management. Wounds uk, 7(2), 54-63.

Richard, J., Daures, J., Parer-Richard, C., Vannereau, D., & Boulot, I. (2000). Of mice and wounds reproducibility and accuracy of a novel planimetry program for measuring wound area. Wounds-A Compendium of Clinical Research and Practice, 12(6), 148-154.

Schäffer, M. R., Tantry, U., & Barbul, A. (2004). Wound fluid inhibits wound fibroblast nitric oxide synthesis. Journal of Surgical Research, 122(1), 43-48. DOI: https://doi.org/10.1016/j.jss.2004.05.009

Swaim, S., Bradley, D., Spano, J., McGuire, J., Hoffman, C., & Trachy, R. (1993). Evaluation of multipeptide-copper complex medications on open wound healing in dogs. The Journal of the American Animal Hospital Association, 29(6), 519-525.

Takehara, K. (2000). Growth regulation of skin fibroblasts. Journal of dermatological science, 24, S70-S77. DOI: https://doi.org/10.1016/S0923-1811(00)00144-4

Taylor, B., & Bayat, A. (2003). Basic plastic surgery techniques and principles: Wound healing. Student British Medical Journal (BMJ), 11(2), 406-407. DOI: https://doi.org/10.1136/sbmj.0308271

Tonnesen, M. G., Feng, X., & Clark, R. A. (2000). Angiogenesis in wound healing. Journal of Investigative Dermatology Symposium Proceedings, 5(1), 40-46. DOI: https://doi.org/10.1046/j.1087-0024.2000.00014.x

Winter, G. D. (1962). Formation of the scab and the rate of epithelization of superficial wounds in the skin of the young domestic pig. Nature, 193(4812), 293-294. DOI: https://doi.org/10.1038/193293a0




How to Cite

Hussin, K. M. A., W, A. S., Hamad, R., & Saleh, M. (2021). Evaluation of Efficacy of Collagen-Calcium Alginate Protectants on Chronic Wound Healing in Horses. Al-Mukhtar Journal of Sciences, 36(2), 116–122. https://doi.org/10.54172/mjsc.v36i2.56



Research Articles