Scrapie in Eastern Libya: Case Report in Sheep
Keywords:Scrapie, Prion, Transmissible Spongiform Encephalopathy, Sheep, Libya
Here we report neuropathological features of scrapie cases detected between 2018 and 2022.
Fourteen cases of sheep older than 2 years from several areas in Libya with neurological clinical signs resembling those with Scrapie were submitted to the Veterinary Teaching Hospital at Omar Al-Mukhtar University / Al-Beida. Postmortem examinations were performed on all animals after euthanasia. Samples from different organs were submitted for histopathological examination. Routine Hematoxylin and Eosin stain (HE) was used.
Nine cases showed neuronal vacuolation that is characteristic of Scrapie in sheep. Two cases were suspected for Scrapie and need more advanced methods such as Immunohistochemistry (IHC) to confirm the diagnosis. The other three cases were diagnosed with Rabies, Listeria, and Echinococcus granulosus.
Arnold, M., & Rajanayagam, B. (2020). Will there be any more classical scrapie cases in sheep in Great Britain? A modelling study to predict future cases. Epidemiology & Infection, 148. DOI: https://doi.org/10.1017/S0950268820001855
Babelhadj, B., Di Bari, M. A., Pirisinu, L., Chiappini, B., Gaouar, S. B. S., Riccardi, G., Vaccari, G. (2018). Prion disease in dromedary camels, Algeria. Emerging infectious diseases, 24(6), 1029. DOI: https://doi.org/10.3201/eid2406.172007
Cassmann, E. D., & Greenlee, J. J. (2020). Pathogenesis, detection, and control of scrapie in sheep. American Journal of Veterinary Research, 81(7), 600-614. DOI: https://doi.org/10.2460/ajvr.81.7.600
Chen, C., Lv, Y., Hu, C., Xu, X.-F., Zhang, R.-Q., Xiao, K., Shi, Q. (2020). Alternative complement pathway is activated in the brains of scrapie-infected rodents. Medical microbiology and immunology, 209(1), 81-94. DOI: https://doi.org/10.1007/s00430-019-00641-6
Farias, G., Frohlich, E., Sanhueza, P., Jara, P., & Lecocq, C. (2017). Differential diagnosis of scrapie in post mortem goats by immuno histo chemistry. Journal of Dairy, Veterinary and Animal Research, 5(4), 121. DOI: https://doi.org/10.15406/jdvar.2017.05.00147
Fast, C., & Groschup, M. H. (2013). Classical and atypical scrapie in sheep and goats. Prions and Diseases, 15-44. DOI: https://doi.org/10.1007/978-1-4614-5338-3_2
Gavier-Widén, D., Stack, M. J., Baron, T., Balachandran, A., & Simmons, M. (2005). Diagnosis of transmissible spongiform encephalopathies in animals: a review. Journal of Veterinary Diagnostic Investigation, 17(6), 509-527. DOI: https://doi.org/10.1177/104063870501700601
Greenwood, P. (2002). Federal disease control—Scrapie. The Canadian Veterinary Journal, 43(8), 625.
Guijarro, I. M., Garcés, M., Andrés-Benito, P., Marín, B., Otero, A., Barrio, T., Monzón, M. (2020). Assessment of glial activation response in the progress of natural scrapie after chronic dexamethasone treatment. International journal of molecular sciences, 21(9), 3231. DOI: https://doi.org/10.3390/ijms21093231
Imran, M., & Mahmood, S. (2011). An overview of animal prion diseases. Virology journal, 8(1), 1-8. DOI: https://doi.org/10.1186/1743-422X-8-493
Konold, T., Spiropoulos, J., Thorne, J., Phelan, L., Fothergill, L., Rajanayagam, B., Coates, N. (2020). The scrapie prevalence in a goat herd is underestimated by using a rapid diagnostic test. Frontiers in bioengineering and biotechnology, 8, 164. DOI: https://doi.org/10.3389/fbioe.2020.00164
Llorens, F., Barrio, T., Correia, Â., Villar-Piqué, A., Thüne, K., Lange, P., Bolea, R. (2018). Cerebrospinal fluid prion disease biomarkers in pre-clinical and clinical naturally occurring scrapie. Molecular neurobiology, 55(11), 8586-8591. DOI: https://doi.org/10.1007/s12035-018-1014-z
Monleon, E., Monzon, M., Hortells, P., Bolea, R., Acin, C., Vargas, F., & Badiola, J. (2005). Approaches to Scrapie diagnosis by applying immunohistochemistry and rapid tests on central nervous and lymphoreticular systems. Journal of virological methods, 125(2), 165-171. DOI: https://doi.org/10.1016/j.jviromet.2005.01.013
Nair, R. R., & Johnson, J. K. (2011). Prions and neuro degenerative diseases. African Journal of Biotechnology, 10(13), 2366-2374.
Saegerman, C., Vanopdenbosch, E., & Berkvens, D. (2007). Current status of scrapie. DOI: https://doi.org/10.1079/PAVSNNR20072027
Schreuder, B. (1994). Animal spongiform encephalopathies‐an update part 1. Scrapie and lesser known animal spongiform encephalopathies. Veterinary Quarterly, 16(3), 174-181. DOI: https://doi.org/10.1080/01652176.1994.9694444
Taranukha, N., Bezgina, Y., Fedota, N., Gorchakov, E., Ozheredova, N., Stepanenko, E., Simonov, A. (2020). Ensuring food security and control of sheep and goat scrapie. E3S Web of Conferences, DOI: https://doi.org/10.1051/e3sconf/202021006020
(OIE,2014)https://wahis.oie.int/#/dashboards/country-or-disease-dashboard (Accessed 8 Feb 2022).
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