Experimental Studies on the effects of Chlorpyrifos on Rats I. Histopathological changes


  • Ibrahim S. H.. El-durssi Zoology Department / College of science / Omar El-Mukhtar University
  • Ifdial O.S. El-Awami Faculty of Agriculture, Plant Protection Department, Omar Almukhtar University, El-Bieda-Libya
  • Ghyath S. mahmoud College of veterinary medicine/Omar El-Mukhtar University
  • Fahim A. Benkhaial Food technology department / college of agriculture / Omar El-Mukhtar University,Food technology department / college of agriculture / Omar El-Mukhtar University
  • Nura Naseb Mohamed College of Medicine / Garyonis University




This Study performed to investigate the effect of Chlorpyrifos (one of the most used insecticides)on certain organs in  rats.

The experimental animals were 110 male rats and were divided into four groups: the first group was used to determine the median lethal dose (LD50) which appeared to be equal to (81.2)mg/Kg B.W.  While the second group of rats received an oral daily doses of 1/10 LD50 . The third and fourth groups of rats  were administered single oral dose of 1/10 LD50 and 1/30 LD50 respectively.

Histopathological findings of the liver revealed a hypertrophy of liver cells with stenosis of the sinusoids, congestion of the blood vessels, necrosis and hemorrhagic spots on the sub capsular spaces. Some hepatic lesions showed fatty changes and liver cirrhosis.

While the pulmonary lesions characterized by pulmonary emphysema and hemorrhage with the presence of hyaline casts in their alveoli.

The spleen showed congestion, lymphoid depletation  and necrosis.

The heart showed hypertrophy of its muscle fibers with the presence of  petechiation on the subendocardial and subepicardial spaces, with Lymphocytic infiltrations.

While sections from the intestine showed hemorrhagic enteritis and slaughing of its epithelial mucosae.

Most of the renal and testicular sections which were examined revealed congestion, exudation and necrosis of their tissues with presences of hyaline casts in the renal tubules.

Sections from the brain showed congestion, exudation, Lymphocytic infiltration and necrosis of Purkinje’s cells, although encephalitis and meningitis were dominant in most animals.


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الحسين نجيب الخطيب ، زيدان هندي عبد الحميد علاء الدين بيومي ، ريم مصطفى زيادة وإيمان محمد عبد المطلب . (2003) .

التأثيرات الهستوباثولوجية لانتقال بعض المبيدات الحشرية عبر الرضاعة في الفئران . المؤتمر العربي الثامن لعلوم وقاية النبات . البيضاء .

غياث صالح محمود (1987) . أمراض الخلية : ترجمة الطبعة الأولى مطبوعات جامعة الموصل ، رقم الإيداع في المكتبة الوطنية ببغداد 623 لسنة 1987م .

El-Sawak, A. A. (1989). Histopathological Changes Due to The Effect of Organophosphorus Insecticides Profenofos in Hens. Egypt. J. Comp. Path. Clin. Path. 139-147.

El-Sawak, A. A., Hussein, Y. A. and El-Manakhly, E. M. (1992). Histopathological Changes in Rats intoxicated with organophosphorus insecticide “Leptophos”. Egypt. J. Comp. Path. Clin. Path.5 (1): 137-149.

Everett, R.W. (1982). Effect of dursban 44 on semen output of Holstein bulls. J. Dairy Sci . 65 : 1781–1794. DOI: https://doi.org/10.3168/jds.S0022-0302(82)82417-X

Hanafy, M. S. M., Arbid, M. S. and Afify, M. M. H. (1989). Biochemical and histopathological effects of the organophosphorus insecticide (Tamaron) in Rats. Egypt. J. Comp. Path. Clin. Path. 2 (2): 251-265.

Lillie, R.D. (1970). Histopathological technique and practical histochemistry . The Blakiston Company , New York , USA .

Mikhail, T. H., Aggour , N., Awadallah, R., Boulos, M.N., EL- Dess-oukey, E.A. and karima, A.I. (1979). Acute toxicity of organophosphorus and organochlorine insecticides in laboratory animals. Z. Ernaehrung swiss 18(4): 258-268. DOI: https://doi.org/10.1007/BF02020516

Soliman, A. A. and El-Zalabani, L. M. (1981). Impairment of spermatogenesis by organophosphorus pesticides. Bull. Alexandria Fac. Med. 17:125-130.

Weil, C. S. (1952). Tables for convention at calculation of median effective dose (LD50 or ED50) and instructions in their use . Biometric 8 : 249-263. DOI: https://doi.org/10.2307/3001557



How to Cite

S. H. El-durssi, I. ., O.S. El-Awami, I. . ., S. mahmoud, G. ., A. Benkhaial, F. ., & Naseb Mohamed, N. . (2006). Experimental Studies on the effects of Chlorpyrifos on Rats I. Histopathological changes. Al-Mukhtar Journal of Sciences, 13(1), 70–82. https://doi.org/10.54172/mjsc.v13i1.668



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