Use of the esterase phenotypes for identification of two Root knot nematodes species, M. incognita and M. javanica for the first time in Libya beside the Pernieal patteren technique

Authors

  • Asma S. W. ALmabrok Plant Protection Department, Faculty of Agriculture, Omar Al-Mukhtar University, El-Beida, Libya
  • Mohammed A.M. Adam Plant Protection Department, Faculty of Agriculture, Omar Al-Mukhtar University, El-Beida, Libya
  • Azzeddin M.Y. Alawam Plant Protection Department, Faculty of Agriculture, Omar Al-Mukhtar University, El-Beida, Libya
  • Mahmoud E. M. Ehwaeti

DOI:

https://doi.org/10.54172/mjsc.v26i1.702

Keywords:

Root knot nematodes, esterase, identification, Libya

Abstract

Root knot disease caused by Meloidogyne spp. is one of the most distributed nematode plant disease in Libya. This genus is comprises of about 80 species. Nine of them were recorded in Libya and their identification was based on morphological characters in spite of the ability of using other accurate and fast methods such as isozyme phenotype and molecular technique which were not used in Libya so far. In this study the usage of detection of esterase enzyme in identification of M. incognita and M. javanica were achieved for the first time in Laboratory of plant protection department, Omar Almuktar University. That will give chance to confirm the identification of the other recorded RKNs species in Libya.

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References

أدم، م. ع. م. (1999). دراسة عن النيماتودا المتطفلة على نباتات العائلة الباذنجانية في منطقة الجبل الأخضر ،رسالة ماجستير ، جامعة عمر المختار ، كلية الزراعة ، قسم الوقاية ، الصفحات 106.

الحازمى، أ. س. (1992). مقدمة في نيماتولوجيا النبات ، جامعة الملك سعود ، الطبعة الأولى ، الصفحات 326.

الحويطى، م. أ. (1989). دراسة نيماتودا تعقد الجذور Meloidogyne spp. على الخضراوات والحشائش في بعض مناطق الجبل الأخضر ، رسالة ماجستير ، كلية الزراعة ، قسم وقاية النبات ، جامعة الفاتح ، طرابلس ، الصفحات 49.

Adam, M. A. M., Phillips, M. S. and Blok, V. C. (2005). Identification of Meloidogyne spp. From North East Libya and comparison of their inter and intra specific genetic variation using RAPDS., Nematology 7(4): 599-609. DOI: https://doi.org/10.1163/156854105774384840

Adam, M. A. M., Phillips, M. S. and Blok, V. C. (2007). Molecular diagnostic key for individual second stage juveniles, females or males of most common and widely distributed species of root knot nematodes (Meloidogyne spp.).Plant Pathology 56,190-197. DOI: https://doi.org/10.1111/j.1365-3059.2006.01455.x

Carneiro, R. M. D. G., Almeida, A. M. R. A. And Carneiro, R. G. (1996). Enzyme phenotypes of Brazilian populations of Meloidogyne spp. Fundamental and applied nematology 19, 555-560.

Dabaj, K. H. and Khan, M. W. (1980). Incidence of root knot disease in tomato and potato and identity of the causal species in the western of the Libyan Jamahiriya Libyan Journal of Agriculture (10): 103-109.

Dickson, D. W., Huisingh, D. and Sasser, J. N. (1971). Dehydrogrnases acid and alkaline phosphates and esterases for chemo taxonomy of selected Meloidogyne, Ditylenchus, Heterodera and Aphelenchus spp,. Journal of nematology (3): 1 16.

Dong, K., Dean, R. A., Forlnum, B. A. and Lewis, S. A. (2001). Development of PCR primers to identify species of root-knot nematodes: Meloidogyne arenaria, M. hapla, M. incognita and M. javanica. Nematropica (31): 271-280.

Eisenback, J. D. (1985). Diagnostioc characters useful in identification of the four most commom species of root knot nematodesw (Meloidogyne spp.). In: Asaaer, J. N. and Carter, C. C.. (Eds) An Advanced Treatisa on Meloidogyne. Vol I. Biology and Control. North Carolina State University Graphics, Raleigh, USA, pp.95-112.

Esbenshade, P. R., and Triantaphyllou, A. C. (1985). Use of enzyme phenotypes for identification of Meloidogyne species. Journal of Nematology (17): 6-20.

Fargette, M. (1987). Use of the asterase phenotype in the taxonomy of the genus Meloidogyne. 1. Stability of the esterase phenotype. Revue de Nematologia (10): 39-43.

Jepson, S. B. (1983). Identification of Meloidogyne species A Comparison of stylets of females. Nematologica (29): 132-143. DOI: https://doi.org/10.1163/187529283X00401

Jepson, S. B. (1987). Identifcation of Root knot Nematodes (Meloidgyne species) CAB International, Wallingford, UK PP. 265.

Karssen, G., Hoenselaar, T. V., Verkerk-Bakker, B. and Janssen, R. (1995). Species identification of cyst and root-knot nematodes from potato by electrophoresis of individual females. Electrophoresis (16): 105-109. DOI: https://doi.org/10.1002/elps.1150160119

Karssen, G. (2002). The plant parasitic nematode genus Meloidgyne Goeldi, 1892 (Tylenchida) in Europe. Brill, Leiden, The Netherlands, pp: 157. DOI: https://doi.org/10.1163/9789004475939

Taylor, A. L. and Sasser, J. N. (1978). Biology identification and control of root knot nematodes (Meloidogyne Species)., Cooperative publication of the Department of plant pathology, North Carolina state University. And U. S. Agency international Development, Raleigh p: 111.

Taylor, D. O. and Netscher, C. (1974). An improved technique for preparing perinal patterns of Meloidogyne spp. Nematologica 20(2): 268 269. DOI: https://doi.org/10.1163/187529274X00285

Trudgill, D. L. and Blok, V. C. (2001). Apomictic polyphagous root knot nematodes: exceptionally successful and damaging biotrophic root pathogens. Annual Review of Phytopathology (39): 53- 77. DOI: https://doi.org/10.1146/annurev.phyto.39.1.53

Whiteh, A. G. (1968). Taxonomy of Meloidogyne (Nematodea, Heteroderidae) with description of four new species Transactions of the Zoological Society of London (31): 263 410. DOI: https://doi.org/10.1111/j.1096-3642.1968.tb00368.x

Zijlsra, C., DonkersVenne, D. T. H. M. and Fargette, M. (2000). Identification of Meloidigyne incognita, M. javanica and M. arenaria using sequence characterized amplififed region (SCAR) based PCR assays. Nematology (2): 847-853. DOI: https://doi.org/10.1163/156854100750112798

Published

2011-12-31

How to Cite

ALmabrok, A. S. W., Adam, M. A., Alawam, A. M., & Ehwaeti, M. E. M. (2011). Use of the esterase phenotypes for identification of two Root knot nematodes species, M. incognita and M. javanica for the first time in Libya beside the Pernieal patteren technique. Al-Mukhtar Journal of Sciences, 26(1), 26–37. https://doi.org/10.54172/mjsc.v26i1.702

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