حصر وتعريف مرض اللفحة النارية على التفاح والكمثري في منطقة الجبل الأخضر- ليبيا

Ngaat I. Omar; Azzeddin M.Y. Alawami; A.M. Adam

doi:10.54172/mjsc.v26i1.703

Authors

Ngaat I. Omar Plant Protection Department, Faculty of Agriculture, Omar Al-Mukhtar University, El-Beida, Libya
Azzeddin M.Y. Alawami Plant Protection Department, Faculty of Agriculture, Omar Al-Mukhtar University, El-Beida, Libya
A.M. Adam Plant Protection Department, Faculty of Agriculture, Omar Al-Mukhtar University, El-Beida, Libya

DOI:

https://doi.org/10.54172/mjsc.v26i1.703

Abstract

This study was carried out during the growth season of apple and pear of the year 2007, to survey and identify the causal agent of fire blight disease in different regions (ELfaidia, Shahat, Elbieda, Elwesita and Gernada) of El-Jabel El-akhdar area. The diagnostic symptoms of this disease included the change of the color of pear and apple flowers to black, pear leaves to black and apple leaves to brown .The ooze was observed on curly pear fruits and finally the fruit was mummified. Results of the survey revealed that the high severity disease was recorded at the study period in ELfaidia region (44.25%) followed by Shahat, Elbieda, Elwesita and Gernada (32.49%). The samples were collected for isolation purposes and the morphological, cultural, biochemical and physiological characters indicated that the causal agent of this disease was the bacterium Erwinia amylovora. Pathgenicity test on premature pear fruits and slices of premature pear and apple fruites confirmed this conclusion. The specific PCR amplification by using specific E. amylovora primers confirmed the identification of these isolates.

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References

أبو غرة ، م .(1997). أمراض النبات البكتيرية ،.منشورات جامعة دمشق . دمشق . 432 صفحة.

أبو غنية ، ع. (1986) . أمراض المحاصيل البستانية . منشورات جامعة الفاتح . طرابلس . 272 صفحة.

أبوبكر ،ع .م . (2005) .دراسات على مرض التبقع البكتيري على نبات الشماري . رسالة ماجستير ، أكاديمية الدراسات العليا . بنغازي .84 صفحة

الهيئة الوطنية للمعلومات و التوثيق. (2004) . مشروع الدراسة المسحية لإعداد خريطة تنموية بشعبية الجبل الأخضر ، الهيئة الوطنية للمعلومات ، البيضاء .46 صفحة

عبد الرحيم ، ع . م . (1996). البكتيريا وأمراض النبات . منشورات جامعة عمر المختار – البيضاء .539 صفحة .

Agrios, G. N.(1997). Plant pathology .4th ed . Academic press , New York, 635 pp.

Al- Dahmashi, M. S. and Khlaif, H.(2004). Fire blight of Pom Fruits in Jordan : disease development and response of different fruit cultivars to the disease Journal of Horticulture Science 10, 81-93. DOI: https://doi.org/10.1016/j.scienta.2003.10.007

Bereswill, S., Bugert, P., Bruchmuller, I. and Geider, K.(1995) .Identification of Erwinia amylovora by PCR with chromosomal DNA. App. Enviromental Microbiology 61, 263 – 2642. DOI: https://doi.org/10.1128/aem.61.7.2636-2642.1995

Billing, E., Crosse, J. E. and Garrett, C. M. E .(1960). Laboratory diagnosis of Fire blight and bacterial blossom blight of pear. Plant Pathology 9, 19.25. DOI: https://doi.org/10.1111/j.1365-3059.1960.tb01706.x

Bradbury, J. F. (1986). Guid to plant pathogenic bacteria CAB international Mycological institute , Ferrylon , Kew surrey . England, 332 pp.

Falkenstein, H., Bellemann, P., Walter, S., Zeller, W. L. and Geider, K. (1988). Identification of Erwinia amylovora , the Fire blight pathogen , by colony hybridization with DNA from plasmid pEA29. Appl. Environomental Microbiology 54, 2798-2802. DOI: https://doi.org/10.1128/aem.54.11.2798-2802.1988

Holt, J. G., Krueg, N. R ., Sneath, P. H., Staley, T. and Williams, S . T. (1994). Bergey's manual of determinative bacteriology. 9th ed . Williams and Wilkins – Blatimore U.S.A., 787 pp.

Horsfall, J. G. and Heuberger, J. W. ( 1942 ). Measuring of a defoliation disease of tomatoes. Phytopathology 32, 226-232.

Kado, C. I. and Heskelt, M. G. (1970). Selective media for isolation of Agrobacterium, Corynebacterium, Erwinia, Pseudomonas, and Xanthomonas. Phytopalthology 60, 969-976. DOI: https://doi.org/10.1094/Phyto-60-969

Kazempour, M., Kamran, E. and Ali, B.(2006). Erwinia amylovora causing fireblight of pear in the Guilan Province of Iran. Journal of Plant Pathology 88 (1), 113-116 .

King, E. O., Word, M. K. and Raney, D. E. (1954). Two simple media for The demonstration of pyocyanin and fluorescein . Journal of Laboratory and Clinial Medicine 44, 301.307.

Kiraly, Z., Klement, Z., Solymosy, F. and Voros, J. (1974). Methods in plant pathology. Elsevier Scientific Publishing Company, Amsterdam, London, New York, 509 pp.

Klement, Z. (1963). Rapid detection of the pathogenicity of pathogenic Pseudomonas. Nature 199, 299-300. DOI: https://doi.org/10.1038/199299b0

Kovacs, N. (1956). Identification of Pseudonmonas pyocyanea by The oxidase reation. Nature. London. 178, 703 . DOI: https://doi.org/10.1038/178703a0

Lelliott, R. A. and Stead, D. E. (1987). Methods for the diagnosis of plant pathogenic bacteria. Black well, scientific publication, London, 216 pp.

Miller, T. D. and Schroth, M. N. (1972). Monitoring The epiphyt population of Erwinia amylovora on pear with a selective medium. Phytopathology 66, 367-370. DOI: https://doi.org/10.1094/Phyto-62-1175

Mohan, S. K. and Schaad, N. W. (1987). Semiselective agar medium for isolating Pseudomonas syringae pv. syringae and pv. Phaseolicola from bean seed. Phytopathology 77, 1390-1395. DOI: https://doi.org/10.1094/Phyto-77-1390

Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular cloning: a laboratory manual. 2nd edition. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, USA, 1659 pp.

Sands, D. C., Schroth, M. N. and Hildebrand, D. C. (1970). Taxonomy of phytopathogenic Pseudomonas. Journal of Bacteria 101, 9-23. DOI: https://doi.org/10.1128/jb.101.1.9-23.1970

Schaad, N. W., Jones, J. B. and Chun, W.( 2001). Laboratory guide for identification of plant pathogenic bacteria .APS Press, 373 pp.

Sholberg, P. L., Bedferd, K. E., Haag, P. and Randall, P. (2001). Survey of Erwinia amylovora isolates from British Columbia for resistance to bactericides and virulence on Apple in Canada. Journal of Plant Pathology 23, 60-67. DOI: https://doi.org/10.1080/07060660109506910

Skerman, V. B. D. (1967). A Guide to the Identification of the Genera of Bacteria, 2nd edition, Baltimore, Maryland: Williams and Wilkins, 303pp.

Stanier, R. Y., Palleroni, N. J. and Doudoroff, M. (1966). The aerobic Pseudomonads : a taxonomic study. Journal of General Microbiology 43, 159-271. DOI: https://doi.org/10.1099/00221287-43-2-159

Vanderzweet, T., Zoller, B. G. and Thomson, S. V. (1988). Controlling Fireblight of pear and apple by accurate prediction of the blossom blight. Plant Disease 72, 464-472. DOI: https://doi.org/10.1094/PD-72-0464