Evaluation of Hepatotoxicity Effect of Sodium  Stibogluconate (Pentostam) in Mice Model

Kaula. A.  Saad; Intisar. O.  Abdalla; Hanan. A.  Alkailani; Ahmed. M.  Elbakush; Zena. A.  Zreiba

doi:10.54172/mjsc.v37i1.738

Authors

Kaula. A. Saad Administration of Zoonotic Diseases Control-National Center of Diseases Control - Tripoli-Libya
Intisar. O. Abdalla Department of Basic Veterinary Medical sciences (Pathology branch)- Faculty of Veterinary Med-icine-Omar Al-Mukhtar University-Albayda-Libya
Hanan. A. Alkailani Department of Basic Veterinary Medical sciences (Pharmacology branch)- Faculty of Veterinary Medicine-Omar Al-Mukhtar University-Albayda-Libya
Ahmed. M. Elbakush Administration of Zoonotic Diseases Control-National Center of Diseases Control - Tripoli-Libya
Zena. A. Zreiba Administration of Zoonotic Diseases Control-National Center of Diseases Control - Tripoli-Libya

DOI:

https://doi.org/10.54172/mjsc.v37i1.738

Keywords:

Leishmaniasis, Pentostam, Hepatotoxicity, Albino Mice

Abstract

The recommended treatment for visceral and cutaneous leishmaniasis is pentavalent antimony at a dosage of 20 mic/kg/day for 28 days. Some studies suggested that antimonial pentostam has multiple acute and chronic adverse effects, which can be minimized by using the lowest effective dose. The present study is aimed to evaluate the hepatotoxicity effect of sodium stibogluconate (pentostam) in mice as a model. Adult male albino mice were divided into three groups, seven mice each, injected with 20 mic/kg pentostam in addition to a control group. Later 14 days, groups II, III, and IV were tested after one, three, and six weeks respecttively. The mice’s serum and liver tissues were collected, and biochemical and histopathological measurement were carried out. Biochemical analysis of the serum obtained showed a significant increase in the levels of AST, ALT, and ALP in groups II and III when compared with the control group. In parallel, the histopathological assessments of the liver tissue proved hepatocytic necrosis. From this study, it can be concluded that the antimonial pentostam has a hepatotoxicity effect on treated mice.

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References

Afshar, S., Farshid, A., Heidari, R., & Ilkhanipour, M. (2008). Histopathological changes in the liver and kidney tissues of Wistar albino rat exposed to fenitrothion. Toxicology and industrial health, 24(9), 581-586. DOI: https://doi.org/10.1177/0748233708100090

Al-Jahdali, M. O., & Bisher, A. S. B. (2007). Testicular histopathological alterations in rats treated with sumithion® NP 25/2.5 EC, insecticide. J. Biol. Sci, 7(3), 520-525. DOI: https://doi.org/10.3923/jbs.2007.520.525

Behrens, R., & Doherty, J. (1993). Severe hepatitis A despite passive immunisation. The Lancet, 341(8850), 972. DOI: https://doi.org/10.1016/0140-6736(93)91274-P

Bergmeyer, H., Horder, M., Rej, R., & Committee, I. F. o. C. C. S. (1986a). Analytical Section: Approved recommendation (1985) on IFCC methods for the measurement of catalytic concentration of enzymes. Part 2. IFCC method for alanine aminotransferase (l-alanine: 2-oxoglutarate aminotransferase, EC 2.6. 1.2). Journal of clinical chemistry and clinical biochemistry. Zeitschrift fÃ¼r klinische Chemie und klinische Biochemie, 24(7), 481-495.

Bergmeyer, H., Horder, M., Rej, R., & Committee, I. F. o. C. C. S. (1986b). Analytical Section: Approved recommendation (1985) on IFCC methods for the measurement of catalytic concentration of enzymes. Part 3. IFCC method for alanine aminotransferase (l-alanine: 2-oxoglutarate aminotransferase, EC 2.6. 1.2). Journal of clinical chemistry and clinical biochemistry. Zeitschrift fÃ¼r klinische Chemie und klinische Biochemie, 24(7), 481-495.

Berman, J. D. (1988). Chemotherapy for leishmaniasis: biochemical mechanisms, clinical efficacy, and future strategies. Reviews of infectious diseases, 10(3), 560-586. DOI: https://doi.org/10.1093/clinids/10.3.560

Dantas-Torres, F. (2006). Leishmune® vaccine: the newest tool for prevention and control of canine visceral leishmaniosis and its potential as a transmission-blocking vaccine. Veterinary parasitology, 141(1-2), 1-8. DOI: https://doi.org/10.1016/j.vetpar.2006.05.001

Elammari, N. E., & Sariti, S. R. (2021). Anti-Leishmanial drug Pentostam induced histological changes to liver and kidney in male BALB/c wild mice. Health Sciences, 1(2), 07-14. DOI: https://doi.org/10.53626/JIMRHS.2021.1202

Galigher, A. E., & Kozloff, E. N. (1971). Essentials of practical microtechnique, Philadelphia, Lea & Fabiger Publisher, 2nd ed.

Gupta, P. S. (1953). Chemotherapy of leishmanial diseases, a resume of recent researches. The Indian Medical Gazette, 88(1), 20.

Harrison, W., Bradberry, S., & Vale, J. (1998). Sodium stibogluconate. UKPID Monograph, UK: National Poisons Information Services,(Birmingham Centre).

Hepburn, N., Siddique, I., Howie, A., Beckett, G., & Hayes, P. (1993). Hepatotoxicity of sodium stibogluconate in leishmaniasis. The Lancet, 342(8865), 238-239. DOI: https://doi.org/10.1016/0140-6736(93)92327-P

Hepburn, N., Siddique, I., Howie, A., Beckett, G., & Hayes, P. (1994). Hepatotoxicity of sodium stibogluconate therapy for American cutaneous leishmaniasis. Transactions of the Royal Society of Tropical Medicine and Hygiene, 88(4), 453-455. DOI: https://doi.org/10.1016/0035-9203(94)90432-4

Killick-Kendrick, R. (1990). The life-cycle of Leishmania in the sandfly with special reference to the form infective to the vertebrate host. Annales de Parasitologie humaine et comparée, 65, 37-42. DOI: https://doi.org/10.1051/parasite/1990651037

Meeting, W. E. C. o. t. C. o. t. L., & Organization, W. H. (2010). Control of the Leishmaniases: Report of a Meeting of the WHO Expert Committee on the Control of Leishmaniases, Geneva, 22-26 March 2010. World Health Organization.

Moss, D. W., & Butterworth, P. J. (1974). Enzymology and medicine. Pitman Medical.

Pearson, R. D., & de Queiroz Sousa, A. (1996). Clinical spectrum of leishmaniasis. Clinical infectious diseases, 1-11. DOI: https://doi.org/10.1093/clinids/22.1.1

Sallie, R., & Tredger, J. (1999). Willaiam. Drugs and the liver. Biopharm Drug Dispos, 12, 251-259. DOI: https://doi.org/10.1002/bdd.2510120403

Thakur, C., Kumar, M., Kumar, P., Mishra, B., & Pandey, A. (1988). Rationalisation of regimens of treatment of kala-azar with sodium stibogluconate in India: a randomised study. Br Med J (Clin Res Ed), 296(6636), 1557-1561. DOI: https://doi.org/10.1136/bmj.296.6636.1557

Tietz, N., Rinker, A., & Shaw, L. (1983). International Federation of Clinical Chemistry. IFCC methods for the measurement of catalytic concentration of enzymes. Part 5. IFCC method for alkaline phosphatase (orthophosphoric-monoester phosphohydrolase, alkaline optimum, EC 3.1. 3.1). IFCC Document Stage 2, Draft 1, 1983-03 with a view to an IFCC Recommendation. Clinica chimica acta; international journal of clinical chemistry, 135(3), 339F-367F.

Vaishwanar, I., Kowale, C., & Jiddewar, G. (1976). Effect Of 2 Ayurvedic Drugs Shilajeet And Eclinol On Changes In Liver And Serum-Lipids Produced By Carbon-Tetrachloride (Vol. 14, pp. 58-61): Council Scientific Industrial Research Publ & Info Directorate, New Delhi ….

Wortmann, G., Miller, R. S., Oster, C., Jackson, J., & Aronson, N. (2002). A randomized, double-blind study of the efficacy of a 10-or 20-day course of sodium stibogluconate for treatment of cutaneous leishmaniasis in United States military personnel. Clinical infectious diseases, 35(3), 261-267. DOI: https://doi.org/10.1086/341406