Histochemical and physiological studies on the Rat's liver during embryonic life and after birth


  • Abdusalam M. Aboalhaj Zoology Department, Faculty of Science, Omar Al-Mukhtar University, Libya.
  • Ebtesam M. M. Gheth Zoology Department, Faculty of Science, Omar Al-Mukhtar University, Libya.
  • Saad M. S. El-Gharbawy Faculty of Veterinary Medicine/ Omar El-Mukhtar University.
  • Ibrahim S.H. El-Durssi Zoology Department, Faculty of Science, Omar Al-Mukhtar University, Libya.




In this study, the development of the rat's liver was investigated during the embryonic life using 39 fetuses ranged from 9-21 days of age, and 39  rats with ages from one day after birth to four months.

The result of the histochemical investigation showing positive Periodic acid schiff (PAS) reaction of hepatic cells from the 20th day of fetal life. These reactions increased with the advancement of age and continued after birth. On the other hand, these hepatic cells showed negative reaction with alcian blue and aldahyed fuchsin stains.                  

Glycogen granules began to appear in the hepatic cells in the last day of intrauterine life. These granules decreased in intensity and quantity in the inner parts of liver as we go far from the capsule. After birth, these granules increased in amount and staining affinity where it distributed allover the liver; in the peripheral and central parts.

There was no difference in the concentration of albumin in all the studied ages.


Download data is not yet available.


أحمد راشد الحميدي , عثمان عبدالله الدوخي و محمد حامد الغندور . (1998) . الأساسيات في عملي أجنة الفقاريات (الوصفي و التجريبي) . الطبعة الأولى . منشورات جامعة الملك سعود . الرياض .

زينب مختار عبد السميع . (2004) . دراسة تأثير المبيد الحشري " كلوربيرفوس" في إحداث التشوهات الخلقية في الجرذان البيضاء . أطروحة ماجستير . كلية العلوم . جامعة عمر المختار . الجماهيرية .

Abdalla, K. E. H. (1997). Prenatal development of the liver in the rabbit. Assiut Vet.. Med. J. 36 (72): 1-21.

Abou-Easa, K. F. K. (1987). Histological and histochemical studieson the liver of developing dromedary Camel (Camelus dromedarius) M. V. Sc. Thesis, Zagazig Univ. (Benha branch).

Anwar, M. E., Hamid, S. H., El-Sayed, E. H. and Zohyd, A. S. E. (1989). A histological study of the postnatal development of the liver of albino rat. Egypt. J. Histol. 12(1): 3-11.

Bancroft, J. D. and Gamble, M. (2002). Theory and practice of histological techniques. Fifth ed. Churchill Livingston. Edinburgh, London and New York.

Cohen, R. L. (1966). Experimental chemoteratogenesis. Adv. Pharmacol. 4: 263-269.

Deane, H. W. (1944). A cytological study of storage and secretion in the developing liver of the mouse. Anat. Rec., 88: 161-174.

Elias, H. (1955). Origin and early development of the liver in various vertebrates. Acta. Hepatol. 3: 1-56.

El-Keshawy, A. H., Awad, A., Abbass, A. and Moustafa, I. A. (1985). Postnatal changes of the liver of female balady rabbitrs in relation of pregnancy and lactation. Zagazig Vet.. J., 12 (2): 360-390.

Fouad, S. M., El-Keshawy, A. H. and Selim, A. (1984). Histological and Histochemical studies of the prenatal development of the liver of One-humped Camel (Camellus dromedarius). Vet.. Med. J. 32 (1): 313-326.

Godlewski, G., Gaubert-Gristol, R., Rowy, S. Prudhomme, M. (1997). Liver development in the rats during the embryonic period (Carnegie Stage 15-23). Acta Anat. 160:172-178.

Hertzberg, C. and Orlic, D. (1981). An electron microscopic study of erythropoiesis in fetal and neonatal rabbit. Acta Anat. 110: 164-172.

Hodgson, E and Levi, P. E. (1997). Textbook of modern toxicology. 2nd. Ed. Applet.on of Lange.

Laemmli,U.k( 1970 ). cleavage of structural proteins during ano, R., Teggi, A.,De Rosa, F. & Vicari, G.(1991). Detection of antibodies against Echinococcus granulosus major antigens and their subunits by immunoblotting. Transactions of the Royal society of Tropical Medicine and Hygiene,85,239-243.

Manson, J. M. Zenick, H. and Costlow, R. D. (1982). Teratology: Test method for laboratory animals. In: principle and method of Toxicology. Student Ed. Edited by Hayes, A. W. Raven press, New York. Pp. 165-182.

Osman, A. H. K., Dougbag, A. S. and Kassem, A. (1984). Organogenesis of the fetal liver of the Egyptian water buffalo (Bos bubalis L.). Egypt. Anat. Soc. 7th. Conference.

Osman, A. H. K., Kassem, A. M. Dougbag, A. S. A. and Moustafa, I. A. (1985). Hemopoiesis in the fetal liver of the Egyptian water Buffalo (Bos bubalis L.) Z. Mikrosk. Anat. Forsch. 99 (2): 219-224.

Severn, C. B. (1972). A morphological study of the development of the human liver. II_ Establishment of liver parenchyma, extra hepatic ducts and associated venous channels. Amer. J. Anat., 133: 85-108.

Yeoh, G. C. T. and Morgan, E. H. (1974). Albumin and Transferrin synthesis during development in the rat. Biochem. J. 144: 215-224.



How to Cite

Aboalhaj, A. M., Gheth, E. M. M., El-Gharbawy, S. M. S., & El-Durssi, I. S. (2008). Histochemical and physiological studies on the Rat’s liver during embryonic life and after birth. Al-Mukhtar Journal of Sciences, 18(1), 175–190. https://doi.org/10.54172/mjsc.v18i1.745



Research Articles