دراسات فسيولوجية ونسيجية على تأثير مستخلص نبات  المرسين على الغدة الدرقية في الجرذان

Noura I.  Al-Zail; Abdusalam M. Aboalhaj; El-Gharbawy Saad M. S.

doi:10.54172/mjsc.v21i1.770

Authors

Noura I. Al-Zail Zoology Department, Faculty of Science, Omar Al-Mukhtar University, Libya.
Abdusalam M. Aboalhaj Zoology Department, Faculty of Science, Omar Al-Mukhtar University, Libya.
El-Gharbawy Saad M. S. Cytology and Histology Department / Faculty of veterinary medicine / Cairo University.

DOI:

https://doi.org/10.54172/mjsc.v21i1.770

Abstract

The aim of this study was to test the effect of the ethanolic extract of Myrtus communis leaves on the thyroid gland tissue in white rats. The animals were given a dose of 2 g/kg orally. 43 rats were divided into 3 groups. The first group was given the dose daily for 7 days. The second group was treated for 14 days . The third group was given the dose for different hours, and after the end of each treatment period, blood samples were taken and the levels of thyroid hormones (T3, T4) and thyroid stimulating hormone (TSH) were determined . Each group included some animals as a control.

Some of the animals treated with the extract showed bleeding through mouth and nose , were lethargic , tired and lost apetite . Furthermore , the weight of these animals was significantly lower than those in the controls . The animals treated with the extract had a significant increase in the levels of TSH , T3 and T4 .

Thyroid gland of treated rats showed an increase in the size of thyroid follicles especially those found at the periphary of the gland . These follicles became irregular and distended by an accumulation of heterogenous colloid with an increase in the amount of vacuoles at the periphary of this colloid . Moreover , some inflamatory cells appeared inside the colloid of some follicles. However , follicular cells appeared compressed with flattened nuclei . Some follicles with ill-distinct boundaries were also appeared.

Downloads

Download data is not yet available.

References

العمامي ، عطية علي أحمد (1999) . تحليل بعض مكونات نوى التمر وتقييمه حيوياً كمشروب ساخن (قنوي) . أطروحة ماجستير . قسم علوم الأغذية . كلية الزراعة . جامعة الفاتح . الجماهيرية الليبية .

القاضي ، عبد الله عبد الحكيم (1954) . استعمالات بعض النباتات في الطب الشعبي الليبي . الجزء الثالث . دار الحكمة للطباعة والنشر والتوزيع . الجماهيرية الليبية . 107-109 .

الكبيسي ، خالد (2002) . الكيمياء الحيوية – العلوم الطبية المساعدة . الطبعة الأولى . دار وائل للنشر والتوزيع . عمان – الأردن .

زايد ، عبد الله عبد الرحمن و توني ، محمد محمد خلف (1998) . علم وظائف الأعضاء – الغدد الصماء والهرمونات . الطبعة الأولى . منشورات جامعة عمر المختار . الجماهيرية الليبية .

شيفيل ، نورمان (1982) . أمراض الخلية . ترجمة : غياث صالح محمود . (1997) . الطبعة الأولى . مطبوعات جامعة الموصل . العراق .

علي ، رابحة عيسى (2007) . تأثير التغذية بالحلبة في بطانة الرحم وبعض المعايير الفسيولوجية لإناث الجرذان . أطروحة ماجستير . قسم علم الحيوان . جامعة عمر المختار . الجماهيرية الليبية .

منسي ، عرسان إرشيد و الشريدة ، محمد شريف (2001) . مقدمة في الكيمياء الحيوية السريرية . الجزء الثاني . دار وائل للطباعة والنشر. عمان – الأردن .

Bancroft, J. D. and Gamble, M. (2002). Theory and Practice of histological techniques. 5th ed. Churchill Livingston. Edinburgh. London & New York.

Bastianetto, S. (2000). Biological activities of the components of Ginkgo biloba. J. Neurochemistry, 74:2268-2277. DOI: https://doi.org/10.1046/j.1471-4159.2000.0742268.x

Bracco, D.; Ferrarra, J. M.; Arnaud, M. J.; Jequier, E. and Schutz, Y. (1995). Effects of caffeine on energy metabolism, heart rate and methylxanthine metabolism in lean and obese women. Am. J. Physiol., 269(4part1):E671-E678. DOI: https://doi.org/10.1152/ajpendo.1995.269.4.E671

Carlton, W. W. and McGavin, M. D. (1995). Special veterinary pathology. 2nd ed. Philadelphia, New York.

Crossmon, G. (1937). A modification of Mallory connective tissue stain with discussion of the principle involved. Ant. Rec., 69:33-38. DOI: https://doi.org/10.1002/ar.1090690105

Deng, Z. Y. and Tao, B. Y. (1998). Effect of green tea and black tea on blood glucose, triglycerides and antioxidants in aged rats. J. Agricult Food Chem., 46:3875-3878. DOI: https://doi.org/10.1021/jf980105+

El-Fellah, M. S.; Akhter, M. H. and Khan, M. T. (1984). Anti-hyperglycaemic effect of an extract of Myrtus communis in streptozotocin-induced diabetes in mice. J. Ethnopharmacol, 11(3):275-281. DOI: https://doi.org/10.1016/0378-8741(84)90073-4

Gartner, R. J. W. and Hurwood, I. S. (1976). The tannin and oxalic acid content of Acacia aneura (mulga) and their possible effects on sulphur and calcium availability. Australian Veterinary Journal, 52:194-195. DOI: https://doi.org/10.1111/j.1751-0813.1976.tb09479.x

Griffiths, D. (1982). The phytate content and iron binding capacity of various field bean (Vicia faba) preparations and extracts. J. Science and Food in Agriculture, 33:847-851. DOI: https://doi.org/10.1002/jsfa.2740330906

Hannan, J. M.; Rokeya, B.; Farugue, O.; Nahar, A.; Mosihuzzaman, M.; Aza-Khan, A. K. and Ali, L. (2003). Effect of soluble dietary fiber fraction of Trigonella foenum-graecum on glycemic, insulinemic, lipidemic and platelet aggregation status of type-2 diabetic model rats. J. Ethnopharmacol, 88:73-77. DOI: https://doi.org/10.1016/S0378-8741(03)00190-9

Mbatha, K. R.; Downs, C. T. and Nsahlai, I. V. (2005). The effects of graded levels of dietary tannin on the epithelial tissue of the gastro-intestinal tract and liver and kidney masses of Boer goats. Med. J., 74(3):3209. DOI: https://doi.org/10.1017/S1357729800052735

Mehansho, H.; Hagerman, A.; Clements, S.; Butler, L.; Rogler, J. C. and Carlson, D. M. (1983). Modulation of proline-rich protein biosynthesis in rat parotid gland by sorghums with high tannin levels. Proc. Nat. Acad. Sci. USA, 80:3948. DOI: https://doi.org/10.1073/pnas.80.13.3948

Mehansho, H.; Rogler, J. C.; Butler, L. G. and Carlson, D. M. (1985). An unusual growth inhibiting effect of tannins on hamsters. Fed. Proc., 44:1960.

Ong, K. C.; Khoo, H. E. and Das, N. P. (1995). Tannic acid inhibits insulin-stimulated lipogenesis in rat adipose tissue and insulin receptor function in vitro. Experientia, 51(6):577-584. DOI: https://doi.org/10.1007/BF02128747

Ott, L. (1984). An introduction to statistical methods and Data Analysis 2nd ed. Duxburg Press, Boston, USA .

Petit, P.; Sauvaire, Y.; Ponsin, G.; Manteghetti, M.; Fave, A. and Ribes, G. (1993). Effect of fenugreek seed extract on feeding behaviour in the rat : metabolic-endocrine correlates. Pharmacol. Biochem. Benav., 45(2):369-374. DOI: https://doi.org/10.1016/0091-3057(93)90253-P

Sato, T.; Onse, Y.; Nagase, H. and Kito, H. (1990). Mechanism of antimutagenicity of aquatic plant extracts against benzo(a) pyrene in the Samonella assay. Mut. Res., 241:283-290. DOI: https://doi.org/10.1016/0165-1218(90)90026-X

Sharma, A. (2006). Comprehensive ayurvedic herbs for superior blood sugar stabilization. Phytotherapy Res., 1-10.

Subon, S. M.; Bilto, Y. Y. and Aburjai, T. A. (2004). Protective effect of selected medicinal plant against protein degradation, lipid peroxidation and deform ability loss of oxidativly stressed human erythrocytes. Phytother. Res., 18(4): 280-284. DOI: https://doi.org/10.1002/ptr.1380