دراسة مقارنة لتمنيع الفئران ضد الأكياس المائية باستخدام المستضد  الإخراجي / الإفرازي للرؤيسات الأولية ومستضد الأكياس المائية :II  نتائج التغير في نسب متوسطات خلايا الدم البيضاء luctuation in average percentage of leukocytes

Fathi M.  Ali; Abdulsalam M. A. Aboelhaj; Waheeda R. Ali

doi:10.54172/mjsc.v21i1.776

Authors

Fathi M. Ali Zoology Department, Faculty of Science, Omar Al-Mukhtar University, Libya.
Abdulsalam M. A. Aboelhaj Zoology Department, Faculty of Science, Omar Al-Mukhtar University, Libya.
Waheeda R. Ali Zoology Department, Faculty of Science, Omar Al-Mukhtar University, Libya.

DOI:

https://doi.org/10.54172/mjsc.v21i1.776

Abstract

Human cystic echinococcosis is a cyclozoonotic infection by larval stage of Echinococcus granulosus . This disease has a world-wide distribution and it causes a big health and economic problems. The difficulties that met scientists in the field are the early diagnosis and treatment of the infection . Therefore , the attentions were directed

toward the possibility of producing a vaccine that has the ability to stimulate the immune response against the infection.

The aim of this work was also directed in this way. Three antigens were extracted and used in our experimental works, These were :

Highly antigenic antigen, the Cyst Fluid (CF) antigen.
Highly antigenic-highly specific antigen, the Excretion–Secretion(ES) antigen .
The third antigen is a mixture of a (1 : 1) proportion of the above two antigens and called ES/CF antigen.

These three antigens were used to immunize the experimental animals (white mice). Three concentrations from each antigen were prepared . The three concentrations were used in white mice in addition to booster dose to stimulate immunity .

The percentage of Neutrophils, Lymphocyte, and Monocytes showed fluctuations, while the percentage of the Eosinophils remained in the normal range after 30 days of the immunization . After 90 days of the injection of the challenge dose, there was a clear depression in the percentage of lymphocytes and clear rising in that of Neutrophilis with fluctuation in the percentage of Monocytes and Eosinophils in the immunized animals that had cysts .

The results in the positive control of mice before the injection of the challenge dose (the same times of the test in the immunized groups) showed that there were no changes in the average percents of white blood cells. However, there were clear differences after 90 days of infection. There was acute depression in the lymphocytes and acute rise in the Neutrophils and a moderate rise in the Eosinophils and Monocytes, the changes sometimes proportional with the infectivity .

The results of the test in infected patients with hydatid cysts showed a clear changes in the average percentages of Eosinophils with a clear increase in these cells , while there were no significant changes in the averages of the other cells.

Downloads

Download data is not yet available.

References

علي ، فتحي محمد (2005). دراسة مقارنه لتمنيع الفئران ضد الأكياس المائية باستخدام المستضد الإبرازي / الإفرازي للروئيسات الأولية ومستضد الأكياس المائية .رسالة ماجستير ، قسم علم الحيوان ، كليه العلوم ، جامعه عمر المختار . البيضاء –ليبيا .

Abdussalam , M; Acha , P.N; Agosin , M; Blood , D.B; Gemmell , M.A. and Kagan , I.G.(1968).

Research needs in Echinococcosis (Hydatidosis ). Bull. WId. HIth .Org., 39 :101- 113.

Ali – Khan , Z .(1974 a) . Host – Parasite relationship in Echinococcosis .I . Parasite biomass and antibody response in three strains of in breed mice against graded doses of Echinococcus multilocularis cysts .J. Parasitol., 60 :231-235. DOI: https://doi.org/10.2307/3278454

Anonymus , S.(1979) .Medical treatment for hydatid disease Br. Med . J.11 : 563. DOI: https://doi.org/10.1136/bmj.2.6190.563

Auer, H. and Aspock , H. (1986). Studies on antigens from in Vitro cultivated protoscolices of Echinococcus multilocularis and their possible use in the serodiagnosis of human Echinococcosis Taeniasis (Cysticercosis ) and Hydatidosis (Echinococcsis ). Second lnt .Symp . (2-7) Dec : 7-15 .

Cox , F.E.G.(1994).Modern Parasitology , 2nd ed. Bluck-Well . Sci. Pul ., Oxford : 194-217 .

Heath , D.D. and Holcman, B.(1997). Vaccination against Echinococcus in perspective. Acta. Trop., 67: 37-41. DOI: https://doi.org/10.1016/S0001-706X(97)00054-5

Heath, D.D.; Parmeter, S.N.; Osborn, P.J. and Lawrence, S.B.(1981). Resistance to Echinococcus granulosus infection in lambs. J. Parasitol., 67: 797-799. DOI: https://doi.org/10.2307/3280702

Kagan, I.G. and Agosin, M.(1968). Echinococcus antigens. Bull. Wld. Hlth. Org., 39: 13-24.

Matossian, R.M.(1977). The immunological diagnosis of human hydatid disease. Trans. Roy Soc. Trop. Med. Hyg., 71 : 101-104. DOI: https://doi.org/10.1016/0035-9203(77)90070-0

Mosimann, F. (1980). Is alveolar hydatid of the liver incurable? Ann. Surg. 192 : 118-123. DOI: https://doi.org/10.1097/00000658-198007000-00021

Okelo, G.B.A. (1986). Hydatid disease : Research and control in Turkana, III. Albendazole in the treatment of inoperable hydatid disease in Kenya -a report on 12 cases Trans. Roy. Soc . Trop. Med. Hyg., 80:193-195. DOI: https://doi.org/10.1016/0035-9203(86)90005-2

Sood, R.(1987). Medical Laboratory technology methods and interpretations. 2nd .ed ., Jaypee Brother Medical Publ., India.