Biological control to some seed born fungal pathogens on some legumes
The biological control using Trichoderma viride and Aspergillus were carried out in vitro on three fungi, Fusarium solani isolated from seeds of local phaseolus variety, Alternaria alternate isolated from seeds of lenties and Stemphylium botryosum isolated from local pea varity. The results show reducing in growth length of pathogenic fungi on PDA media due to the presence of antagonistic fungi. Under green house conditions the biological control using the mycorrhizae (A-Glomus intraradices and B-G. ctunicatium) , antagonistic fungus T viride and the bacterium Rhizobium sp. rev.ealed general reducing in death ratios of seed before and after germination and also reducing the infection rates. Using the mycorrhizae A and B in treatment seeds of Safsaf lenticels showed the presence of significant differences between the treatments in fresh and dry weight of vegetative growth and in the length and weight of fresh root system. During the use of 7: viride significant differences were observed between the treatments in length of vegetative growth, in fresh and dry weight of vegetative growth and in length and weight of fresh root system. From the results of using the Rhizobium SF. significant differences were observed between the treatments and revealed increasing in the length and weight of vegetative and root system and number of root nodules as compared with control. Results of using mycorrhizae A and B as biological control agent against F. solani on seeds of local bean showed increasing in length, fresh and dry weight of vegetative and root system. Using of T viride against F. solani on local bean showed also increasing in length, fresh and dry weight of vegetative and root system. using mycorrhizae A and B, T viride and Rhizobium as biological control agent against Stemphylium on seeds of local pea lead to increasing in length, fresh and dry weight of vegetative and root system. The infection percent of endomycorrhizae on root of local bean, local pea and Safsaf lenticels was 100%.
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