Effect of sucrose, pH and medium states on in vitro shoot formation and growth of Moris pineapple (Ananas comosus L. Merr.)
DOI:
https://doi.org/10.54172/mjsc.v32i1.92Keywords:
Sucrose concentration, pH; Pineapple, Ananas comosus, Media statesAbstract
The effect of medium states (solid, semi solid and liquid) of full strength MS medium enriched with 6-benzylaminopurine (BAP) at 2.0 mg/l on in vitro shoots formation and shoot length of Moris pineapple were tested at 16 combinations of sucrose (10, 20, 30 and 40 g/l) and pH (5.0, 5.7, 6.0 and 6.5). The highest shoot formations (7 shoots/ explant) were obtained in liquid and solid media each adjusted to pH 5.0 but enriched with different sucrose concentrations, sucrose at 20 g/l for liquid and at 30 g/l for solid medium. Increasing the medium sucrose to 40 g/l or adjusting the medium to pH 6.0 caused 50 % decline in the shoot formation capacity in both medium states. However, while that decline could be reversed in liquid medium by adjusting the pH to 6.5, such pH adjustment failed to overcome the inhibitory effect of the sucrose at 40 g/l in the solid medium. Out of 16 combinations of sucrose and pH, liquid medium (no agar added) was better than solid (7.0 grams of agar /l) and semi solid (3.5 grams of agar /l) at 8 combinations, equal to solid at 4 and to semi solid at 5 combinations and less than solid at 3 and than semi solid at 2 combinations. Adopting of the commonly used combination of sucrose at 30 g/l and pH 5.7 not only did not fit all medium states but also resulted in lower shoot formation (4 shoots) than the possibly obtainable (7 shoots). Simple modification of the medium pH (pH 5.0 instead of 5.7) doubled the rate of shoot formation.
Downloads
References
Akin-Idowu, P. E., Akinyemi S. O. S., and Ibitoye D. O. (2014). Influence of medium type and growth regulators on in vitro micropropagation of pineapple (Ananas comosus (L.), var. Smooth cayenne). African Journal of Plant Science 8(9):450-456. DOI: https://doi.org/10.5897/AJPS2014.1184
Almeida, W. A. B. D., Santana G. S., Rodriguez A. P., and Costa M. A. P. D. C. (2002). Optimization of a protocol for the micropropagation of pineapple. Revista Brasileira de Fruticultura 24(2):296-300. DOI: https://doi.org/10.1590/S0100-29452002000200005
Escalona, M., Lorenzo J., González B., Daquinta M., González J., Desjardins Y., and Borroto C. (1999). Pineapple (Ananas comosus L. Merr) micropropagation in temporary immersion systems. Plant Cell Reports 18(9):743-748. DOI: https://doi.org/10.1007/s002990050653
Fernando, K. (1986). In Vitro propagation of mauritius pineapple. Tropical Agricult. (Sri Lanka).
Firoozabady, E., and Gutterson N. (2003). Cost-effective in vitro propagation methods for pineapple. Plant Cell Reports 21(9):844-850. DOI: https://doi.org/10.1007/s00299-003-0577-x
Hamad, A. M., and Taha R. M. (2008). Effect of sequential subcultures on in vitro proliferation capacity and shoot formations pattern of pineapple (Ananas comosus L. Merr.) over different incubation periods. Scientia Horticul. 117(4):329-334. DOI: https://doi.org/10.1016/j.scienta.2008.05.009
Kofi, O., and Adachi T. (1993). Effect of cytokinins on the proliferation of multiple shoots of pineapple in vitro. SABRAO Journal 25(1):59-69.
Mathews, V. H., and Rangan T. (1979). Multiple plantlets in lateral bud and leaf explant in vitro cultures of pineapple. Scientia Horticul. 11(4):319-328. DOI: https://doi.org/10.1016/0304-4238(79)90016-5
Pérez, A., Napoles L., Carvajal C., Hernandez M., and Lorenzo J. (2004). Effect of sucrose, inorganic salts, inositol, and thiamine on protease excretion during pineapple culture in temporary immersion bioreactors. In Vitro Cellular and Developmental Biology-Plant 40(3):311-316. DOI: https://doi.org/10.1079/IVP2004529
Pérez, G., Yanes E., Isidrón M., and Lorenzo J. C. (2009). Phenotypic and AFLP characterization of two new pineapple somaclones derived from in vitro culture. Plant Cell, Tissue and Organ Culture 96(1):113-116. DOI: https://doi.org/10.1007/s11240-008-9463-0
Pérez, G., Yanez E., Mbogholi A., Valle B., Sagarra F., Yabor L., Aragón C., González J., Isidrón M., and Lorenzo J. C. (2012). New pineapple somaclonal variants: P3R5 and Dwarf. Am J Plant Sci 3(1-11. DOI: https://doi.org/10.4236/ajps.2012.31001
Soneji, J. R., Rao P., and Mhatre M. (2002a). In vitro regeneration from leaf explants of pineapple (Ananas comosus L, Merr). Journal of plant biochemistry and biotechnology 11(2):117-119. DOI: https://doi.org/10.1007/BF03263147
Soneji, J. R., Rao P., and Mhatre M. (2002b). Somaclonal variation in micropropagated dormant axillary buds of pineapple (Ananas comosus L., Merr.). The Journal of Horticultural Science and Biotechnology 77(1):28-32. DOI: https://doi.org/10.1080/14620316.2002.11511452
Sripaoraya, S., Marchant R., Brian Power J., and Davey M. R. (2003). Plant regeneration by somatic embryogenesis and organogenesis in commercial pineapple (Ananas comosus L.). In Vitro Cellular and Developmental Biology-Plant 39(5):450-454. DOI: https://doi.org/10.1079/IVP2003445
Teixeira, S. L., Ribeiro J. M., and Teixeira M. T. (2006). Influence of NaClO on nutrient medium sterilization and on pineapple (Ananas comosus cv Smooth cayenne) behavior. Plant Cell, Tissue and Organ Culture 86(3):375-378. DOI: https://doi.org/10.1007/s11240-006-9121-3
Downloads
Published
How to Cite
License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Copyright of the articles Published by Almukhtar Journal of Science (MJSc) is retained by the author(s), who grant MJSc a license to publish the article. Authors also grant any third party the right to use the article freely as long as its integrity is maintained and its original authors and cite MJSc as original publisher. Also they accept the article remains published by MJSc website (except in occasion of a retraction of the article).
