تأثير عقار البروكايين Procaine على النشاط الميكانيكي والانزيمي للعضلات الملساء للفائفي الجرذ

Khalid H. M Saad

doi:10.54172/mjsc.v22i1.1049

Authors

Khalid H. M Saad Department of Zoology, Faculty of Science, Omar Al-Mukhtar University, Derna-Libya

DOI:

https://doi.org/10.54172/mjsc.v22i1.1049

Keywords:

Procaine, smooth muscle, rat

Abstract

These experiments were a trial to find out the effect of procaine on the spontaneous rhythmic contraction and on the enzyme activity of the mitochondria and membrane vesicles of the smooth muscle of the rat ileum. Procaine has been used at different levels of concentrations. It was found that the very low and low concentrations(O.OOlto O.SmM) increase the tone and enhance the rhythmic spontaneous contractions, mean while concentrations of the drug (3-SmM) induced contracture and reach its maximum level at 8mM of Procaine. Use of very high concentrations (10 -lSmM) caused reverse effect; it induced immediate relaxation to the normal spontaneous contractions. These results might indicate that the procaine exerted its action on this muscle by its interaction with Ca²+ influx through the cell membrane. These hypotheses is confirm.ed by the effect of the drug on the enzyme activity where the drug in low and moderate concentrations enhance the enzyme activity whereas high concentration inhibited the activity

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References

Aceves J. and Machne X. (1963) . The action of Caz+ and of anesthetics on nerve cells and their interaction during excitation .J. pherme. exp their 140,138 -148 .

AI . Badran , A . I. (1989). The effect of quanine on the mechanical activity and Oz concentration of ileal smooth muscle. of Golden hamster and laboratory mice, Msc Univ. Salahddin

•Blaustein, M. P. and Goldman, D. E (1966).Competitive action of Caz+ and procaine on lobster axon .A study of mechanism of action of certain local anesthetics .J Gen. Physiol49 ; 1053 -1063. DOI: https://doi.org/10.1085/jgp.49.5.1043

Brading, A. , Bulbring, E. ,and Tomita, T. (1969).The effect of temperature on the membrane conductance of the smooth muscle of guinea pig taenia coli. J. Physiol. ,200 : 621 - 635 . DOI: https://doi.org/10.1113/jphysiol.1969.sp008712

Bulbring E and Tomita,T.(l970) .Effect of Caz+ removal on smooth muscle. of Guinea pig taenia coli. J. Physiol London, 210, 217 - 232. DOI: https://doi.org/10.1113/jphysiol.1970.sp009205

Bulbring E. and Kuviyama H. (1963). Effects of changes in the external Na and Ca concentration on spontaneous electrical activity in smooth muscle of guinea pig taenia coli. J. physiol .166,29. 58 . DOI: https://doi.org/10.1113/jphysiol.1963.sp007089

Endo, M. (1977).Caz+ release from the sacroplasmic reticulum. Physiol . Rev. 57 ,71 -108 . DOI: https://doi.org/10.1152/physrev.1977.57.1.71

Feinstein ,M.B. (1966). Inhibition of contraction and Caz+ exchangeability in rat uterus by local anesthetics. J. Pharmacol. Exp. Ther. 152 ,516 - 524.

Feinstein ,M.B. and Paimer ,M.(1969).Pharmacological action of local anesthetics on excitation - contraction coupling in striated and smooth muscle. Fed. Proc, 28: 1643 -1648 .

Gabella, G. and Raeymaekers, I. (1976) .Effect of collagenase on mechanical activity and fine structure of an intestinal smooth muscle. Cell Tissue. Res. 173,29 -169. DOI: https://doi.org/10.1007/BF00219264

Jurgen W.G.E., Van Teeffelen and Steven S .Segal (2007) Rapid dilation of of arterioles with single contraction of hamster skeletal muscle. Am ,J physiol Heart Circ 292: H2764-H 2772 .

Imai, S. , Nakazawa, H. and Nabata, H.(1984).Effect of procaine on the isolated dog coronary A. Archives Internationals de pharmacodynamicet de Therapie vol-271 No 1:99 -105.

Imai,S.and Takeda ,K. (1976). Action of Caz+ and certain multivalent action on K+ contracture of guinea - Pigs taenia Coli.J.Physiol.,190:155¬169. DOI: https://doi.org/10.1113/jphysiol.1967.sp008199

Kurihara ,S. (1973). The effect of procaine on the urinary bladder smooth muscle of built frog. Jap. J. physiol, 23 :309 - 424 . DOI: https://doi.org/10.2170/jjphysiol.23.309

Kurihara, S.(1975) .The effect of procaine on the mechanical and electrical activities of the smooth muscle cells of the guinea Pig urinary bladder. Jap. J. 25 :775 - 788 . DOI: https://doi.org/10.2170/jjphysiol.25.775

Kurihara,S. and Sakai T. (1976). Relationship between effects of Procaine and Ca2+ on spontaneous electrical and mechanical activities of the smooth muscle cells of guinea pig urinary bladder. DOI: https://doi.org/10.2170/jjphysiol.26.487

Kuriyama ,H.,K. Kitamura,T. Itoh and R. Inoue,(1998). Physiological Features of Visceral smooth muscle cells, with special reference to Receptors and Ion channels. Physiol.Rev. 78:811 - 920. DOI: https://doi.org/10.1152/physrev.1998.78.3.811

Magarbuchi, T. ,Ito, Y., and Kuriyama, H.(1973).Effects of rapid cooling on the mechanical and electrical activities of smooth muscle of guinea pig smooth muscle and taenia coli. J. Gen. Physiol , 61 ,323-341. DOI: https://doi.org/10.1085/jgp.61.3.323

Saad, K. H.M. (1980). Ca2+ regulation during excitation- Contraction coupling of mammalian smooth muscle Ph.D. thesis. University of Lancaster ,UK.

Saad ,K.H.M(2008) Alink between the effect of procaine on oxygen consumption and its effect on the mechanical activities of rat ileum smooth muscle . Journal of Science and its application. in the press.

Sakai ,T. and Iizuka ,T.(1972). The effect of caffeine and rapid cooling on smooth muscle. Jap. J. physiol;22:135 -145. DOI: https://doi.org/10.2170/jjphysiol.22.135

Shanes ,A.M., Freygang ,W. H., Grundfest, H. ,and Amatriek, E.(l959). Anesthetic and Ca2+ action in the voltage clamped squid giant axon. J. Gen. Physiol , 42: 795 - 802. DOI: https://doi.org/10.1085/jgp.42.4.793

Urakawa , N. and Holand ,W.e. (1964).Ca45 uptake and tissue Ca in K-induced phasic and tonic contraction in taenia coli. Am. J. physiol, 207: 873 - 876. DOI: https://doi.org/10.1152/ajplegacy.1964.207.4.873

Washizuy . (1968b). Stomtium and barium ions in guinea pig ureter. Comp Biochem. Physiol25 ,367 - 371. DOI: https://doi.org/10.1016/0010-406X(68)90947-X

Yoshii, K. Iizuka, K. Dobashi, T. Horie, T. Harada, T. Nakazawa and M. Mori, (1999). Relaxation of conracted Rabbit Tracheal and Human Bronchial smooth muscle byY - 27632 through Inhibition of Ca2+ sensitization. Am. J. Respir. Cell Mol. BioI., 20(6) : 1190 -1200 DOI: https://doi.org/10.1165/ajrcmb.20.6.3441

سعيد، خالد حميد محمد (1998) تأثير عقار الكافيين على النشاط الذاتي والتقلص التوتري المنتج بالاستايل كولين في العضلات الملساء للفائفي الجرذ. مجلة المختار للعلوم، العدد الخامس ص97-110. DOI: https://doi.org/10.54172/mjsc.v5i1.565