Investigation of EMS effect on Vicia faba seed germination and seedling growth under saline condition

Khaled  Elmeer; Eman  Abduljawad; Ebtisam  Eljrary

doi:10.54172/mjsc.v31i1.215

Authors

Khaled Elmeer Horticulture Dep., Faculty of Agriculture. Omar Al-Mukhtar University, Al Bayda, Libya
Eman Abduljawad Plant production Dep., Faculty of Agriculture, Benghazi University, Benghazi, Libya
Ebtisam Eljrary Plant production Dep., Faculty of Agriculture, Benghazi University, Benghazi, Libya

DOI:

https://doi.org/10.54172/mjsc.v31i1.215

Keywords:

Broad bean, EMS, NaCl

Abstract

The effect of Ethyl-methane Sulphonate (EMS) on seed germination and seedling characters of broad beans (Vicia faba L.) was analyzed in this investigation. The seeds were soaked in different percentages and durations of (EMS), then after sowing were irrigated with a range of NaCl concentration. The effect of EMS was observed based on the seed germination (%), and seedling characters such as: total fresh weight, root fresh weight and root length.

Germination percentage generally decreases while concentrations of NaCl increases, however the interaction effect of EMS clearly appeared on concentration 6000 ppm NaCl, which positively increased the germination from 40% in absence of EMS to 60%, when 0.5 or 1% EMS were used. 24 hours soaking in EMS decreased the fresh weight from 33.36 to 13.55 gm per plant, in addition to the decrease of the root length from 22.0cm to 14.2cm per plant. The root nodulation of broad bean is not affected during the increase of NaCl, possibly due to the interactional effect of mutagenesis agent.

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References

Akhtar, N. (2014). Effect of physical and chemical mutagens on morphological behavior of tomato (Lycopersicon esculentum L.,) CV. “Rio Grande” under heat stress conditions. Scholarly Journal of Agricultural Science, 4:350-355.

Almansouri, M., J. M. Kinet and S. Lutts. (2001). Effect of salt and osmotic stresses on germination in durum wheat (Triticum durum Desf.). Plant and Soil, 231:243-254. DOI: https://doi.org/10.1023/A:1010378409663

Ananthaswamy, H. N., U. K. Vakil and A. Sreenivasan. (1971). Biochemical and physiological changes in gamma-irradiated wheat during germination. Radiation Botany, 11:1-12. DOI: https://doi.org/10.1016/S0033-7560(71)91257-9

Anbarasan, K. and R. Rajendran. (2013). Studies on the mutagenic effect of EMS on seed germination and seedling characters of Sesame (Sesamum indicum L.) Var. TMV3. International Journal of Research in Biological Sciences, 3:68-70.

Bhat, T. A., A. H. Khan, S. Parveen and F. A. Ganai. (2005). Clastogenic effect of EMS and MMS in Vicia faba L., Journal of Cytology and Genetics, 6:117-122.

Bhat, T. A., M. Sharma and M. Anis. (2007). Comparative Analysis of Meiotic Aberrations Induced by Diethylsulphate and Sodium Azide in Broad Bean (Vicia faba L.). Asian Journal of Plant Sciences, 6:1051-1057. DOI: https://doi.org/10.3923/ajps.2007.1051.1057

Bibi, A., N. Ellahi, A. Ali, F. Hussain, N. Hussain and M. Ahmad. (2012). Nutritional Influence of Salt Stress on the Growth and Nodule Formation of Vicia faba. Pakistan Journal of Nutrition, 11:562-567. DOI: https://doi.org/10.3923/pjn.2012.660.665

Bliss, R. D., K. A. Platt-Aloia and W. W. Thomson. (1986). Osmotic sensitivity in relation to salt sensitivity in germinating barley seeds. Plant Cell Environment, 9:721-725. DOI: https://doi.org/10.1111/j.1365-3040.1986.tb02104.x

Bökel, C. (2008). EMS screens: from mutagenesis to screening and mapping. Methods in Molecular Biology, 420:119-138. DOI: https://doi.org/10.1007/978-1-59745-583-1_7

Bond, D.A., D.A. Lawes, G.C. Hawtin, M.C. Saxena and J.S. Stephens. (1985). Faba bean (Vicia faba L.). In: Grain Legume Crops, Ed.: R. J. Summerfield and E. H. Roberts, William Collins & Sons, London, U.K., P: 199-265.

Chrispeeds, M. J. and J. E. Varner. (1976). Gebberllic acid induced synthesis and release of α-analysis and ribonuclease by isolated barley aleurons layers. Plant Physiology. 42:346-406. DOI: https://doi.org/10.1104/pp.42.3.398

Cooper, J. E., M. Wood and A. J. Bjourson. (1986). Influence of acidity and aluminium on the nodulation of marsh trefoil and white clover by rhizobia. In: Microbial Communities in Soil, Ed.: V. Jensen, A. Kjoller and L. H. Sorensen, Elsevier Scientific Publishing Company, Oxford, U.K., P: 97-104

Dhanayanth, K. P. M. and V. R. K. Reddy. (2000). Cytogenetic effect of gamma says and ethylmethane sulphunate in chilli piper (Capsicum annuum). Cytologia, 65:129-133. DOI: https://doi.org/10.1508/cytologia.65.129

Epstien, E. and J. D. Norlyn. (1997). Sea water based crop production: A feasibility study. Science, 197: 249- 251. DOI: https://doi.org/10.1126/science.197.4300.249

Ganesan J. (1998). Induced mutations for sesame improvement. Proc. Third FAO/ IAEA Res. Co-ord. Meet. On induced mutations for sesame improvement. 6-10 April, Bangkok, Thailand.

Goyal, S. and S. Khan. (2010). Induced mutagenesis in urdbean: A review. International Journal of Botany, 6: 194-206. DOI: https://doi.org/10.3923/ijb.2010.194.206

Joshi, N., A. Ravindran and V. Mahajan. (2011). Investigation on chemical mutagen sensitivity in onion. International Journal of Botany, 7: 243-248. DOI: https://doi.org/10.3923/ijb.2011.243.248

Katerji N., I. W. van Hoorn, A. Hamdy, N. Bouzid, M. S. El Sayed and M. Mastrorilli. (2002). Effect of salinity on water stress, growth and yield of broadbean. In: Mediterranean crop responses to water and soil salinity: Eco-physiological and agronomic analyses, Ed.: N. Katerji, A. Hamdy, I. W. van Hoorn and M. Mastrorilli, Bari: CIHEAM, P: 1-15.

Kleinhofs, A., R. L. Warner, F. J. Muehlbauer and R. A. Nilan. (1978). Induction and selection of specific gene mutations in Hordeum and Pisum. Mutation Research, 51: 29–35. DOI: https://doi.org/10.1016/0027-5107(78)90005-2

Kozgar, M. I., S. Goyal and S. Khan. (2011). EMS induced mutational variability in Vigna radiate and Vigna mungo. Research Journal of Botany, 6: 31-37. DOI: https://doi.org/10.3923/rjb.2011.31.37

Kumar, A. and M. N. Mishra. (2004). Effect of gamma rays, EMS and NUM on germination, seedling vigor, pollen viability and plant survival in M1 and M2 generation of Okra (Ablemoschus esculentus L.) Moench. Advances in plant sciences, 17: 295-297.

Kumar, G., and R. S. Yadav. (2010). EMS induced genomic disorders in sesame. Romanian Journal of Biology - Plant Biology, 55: 97-104.

Lal, R. K. (1985). Effect of salinity applied at different stages of growth on seed yield and its constituents in field peas (Pisum sativum L. var. arvensis). Indian Journal of Agronomy, 30: 296-299.

Lauter, D. J., D. N. Munns and K. L. Clarkin. (1981). Salt response of chickpeas influenced by N supply. Agronomy journal, 73: 961-966. DOI: https://doi.org/10.2134/agronj1981.00021962007300060013x

Lima, J. M., M. Nath, P. Dokku, K. V. Raman, K. P. Kulkarni, C. Vishwakarma, S. P. Sahoo, U. B. Mohapatra, S. V. Amitha Mithra, V. Chinnusamy, S. Robin, N. Sarla, M. Seshashayee, K. Singh, A. K. Singh, N. K. Singh, R. P. Sharma and T. Mohapatra. (2015). Physiological, anatomical and transcriptional alterations in a rice mutant leading to enhanced water stress tolerance. AoB PLANTS :1-48. DOI: https://doi.org/10.1093/aobpla/plv023

Micke, A. (1983). Some considerations on the use of induced mutations for improving disease resistance of crop plants. In: Proc. Res. Meet. IAEA, Riso, Denmark. pp. 3-19.

Micke, A. (1988). Genetic improvement of grain legumes using induced mutations. In: Improvement of Grain Legumes Production Using Induced Mutations. 4, International Atomic Energy Agency (IAEA), Vienna. pp. 1-8

Mirza, J. I. and R. Tariq. (2011). The growth and nodulation of Vicia faba as affected by salinity. Biologia Plantarium, 35: 289-292. DOI: https://doi.org/10.1007/BF02925957

Predeepa, R. J. and D. A. Ravindran. (2010). Nodule formation, distribution and symbiotic efficacy of Vicia faba L. under different soil salinity regimes. Emirates Journal of Food and Agriculture, 22: 275-284. DOI: https://doi.org/10.9755/ejfa.v22i4.4875

Sato, M. and H. Gaul. (1967). Effect of ethyl methane sulphonate on the fertility of barley. Radiation Botany, 7: 7-15. DOI: https://doi.org/10.1016/0033-7560(67)90028-2

Shah, T. M., J. I. Mirza, M. A. Haq and B. M. Atta. (2008). Radio sensitivity of various chickpea genotype in M1 generation. Pakistan Journal of Botany, 40: 649-665.

Shokohifard, G., K. H. Sakagam and S. Matsumoto. (1989). Effect of amending materials on growth of radish plant in salinized soil. Journal of Plant Nutrition, 12: 1195-1294. DOI: https://doi.org/10.1080/01904168909364031

Sprent, J. I. (1984). Nitrogen fixation. In: Advances in plant physiology, Ed.: M.B Wilikins, Pitman, London, P: 249-276.

Sri Devi, A. and L. Mullainathan. (2011). Genotoxicity Effect of Ethyl Methanesulfonate on Root Tip Cells of Chilli (Capsicum annuum L.) World Journal of Agricultural Sciences, 7: 368-374.

Talebi, A. B., A. B. Talebi and B. Shahrokhifar. (2012). Ethyl Methane Sulphonate (EMS) Induced Mutagenesis in Malaysian Rice (cv. MR219) for Lethal Dose Determination. American Journal of Plant Sciences, 3: 1661-1665. DOI: https://doi.org/10.4236/ajps.2012.312202

Tarar, J. L. and V. R. Dnyansagar, (1980): Comparison of ethyl methane sulphonate and radiation induced meiotic abnormalities in Turnera ulmifolia L. var angustifolia wild. Cytologia, 45: 221-231. DOI: https://doi.org/10.1508/cytologia.45.221

Yousaf, A. N. and J. I. Sprent. (1983). Effects of NaCl on growth, chemical composition of inoculated and NH4NO3 fertilized Vicia faba L. plants. Journal of Experimental Botany, 34: 941-950. DOI: https://doi.org/10.1093/jxb/34.8.941

Zeerak, N. A. (1991). Cytogenetical effect of gamma rays and ethylmethane sulphunate in brinjal (Solanum Melongena L.). Cytologia, 56: 639-643. DOI: https://doi.org/10.1508/cytologia.56.639