Effect of Incubation Periods, Medium Volumes and Explants Density on in Vitro Shoot Formation and Growth and Cost of Multiplication of Moris Pineapple (Ananas comosus L.)

Abdelhamid A.  Hamad

doi:10.54172/mjsc.v33i2.290

Authors

Abdelhamid A. Hamad Department of Horticulture, Faculty of Agriculture, Omar Al-Mukhtar University, El-Bayda, Libya

DOI:

https://doi.org/10.54172/mjsc.v33i2.290

Keywords:

Total shoots, Total cost, Medium volume, Explants density, Incubation periods, Pineapple, Ananas comosus

Abstract

This study was conducted to investigate the effect of combinations of four incubation periods (30, 45, 60 and 75 days), three explants density (one, two and three) and four volumes of medium (3, 6, 9 and 12 ml) in the in vitro shoot formation per explant, total shoots and total cost per liter of medium and cost per shoot of Moris pineapple. The highest shoot per explant was obtained in combination of three explants in 12 ml of medium incubated for 60 days, three explants in 9 and in 12 ml of medium and one explant in 3 and in 9 ml of medium incubated for 75 days but at different total shoots (2750, 3667, 2750, 3667 and 1222 shoots), total cost ( USA $25.85, 36.09, 28.39, 95.32 and 36.06) per liter of medium. Combinations in which three explants were used per culture resulted in lowest cost per shoot (1.0 cent) while combination in which one explant was used per culture, the cost per shoot was two to three times higher (2.6 and 2.9 cents). Combination of three explants, 3 ml of medium and 75 days of incubation, on the other hand, resulted in formation of only 6 shoots per explant (almost half of the highest obtained rate) but in highest total shoot per liter of medium (6000 shoots ) at highest total cost (USA $95.32) and intermediate cost per shoot (1.6 cents). Combination of three explants, 6 ml of medium and 75 days resulted in formation of 10 shoots per explant, production of 5000 shoots per liter of medium at lowest cost per shoot (1.0 cent) and intermediate total cost per liter of medium (USA $ 50.68). Using of one explant in 12 ml for 30 days resulted in formation of 5 shoots per explant, highest cost per shoot (5.0 cents) and lowest total shoots (417 shoots) and lowest total cost (USA $ 20.73) per liter of medium.

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References

Almeida, W. A. B. Santana G. S. Rodriguez A. P. and Costa M. A. and De C. (2002). Optimization of a protocol for the micropropagation of pineapple. Revista Brasileira de Fruticultura 24(2):296-300. DOI: https://doi.org/10.1590/S0100-29452002000200005

Be, L. and Debergh P. (2006). Potential low-cost micropropagation of pineapple (Ananas comosus). South African Journal of Botany 72(2):191-194. DOI: https://doi.org/10.1016/j.sajb.2005.07.002

Bhatia, P. and Ashwath N. 2002. Development of a rapid method for micropropagation of a new pineapple [Ananas comosus (l.) Murr.] clone,'Yeppoon gold'. Pages 125-131 in International Symposium on Tropical and Subtropical Fruits 575. DOI: https://doi.org/10.17660/ActaHortic.2002.575.11

Dal Vesco, L. L. de Almeida Pinto A. Zaffari G. R. Nodari R. O. dos Reis M. S. and Guerra M. P. (2001). Improving pine-apple micropropagation protocol through explant size and medium composition manipulation. Fruits 56 (3):143-154. DOI: https://doi.org/10.1051/fruits:2001100

Daquinta, M. Cisneros A. Rodriguez Y. Escalona M. Perez M. Luna I. and Borroto C. (1997). Somatic embryog -enesis in pineapple (Ananas comosus (L.) Merr). Pages 251-258 in II International Pineapple Symposium 425. DOI: https://doi.org/10.17660/ActaHortic.1997.425.28

Duncan, D. B. (1955). Multiple range and multiple F tests. Biometrics 11(1):1-42. DOI: https://doi.org/10.2307/3001478

Escalona, M. Lorenzo J. González B. Daquinta M. González J. Desjardins Y. and Borroto C. (1999). Pineapple (Ananas comosus L. Merr) micropropa -gation in temporary immersion systems. Plant cell reports 18(9):743-748. DOI: https://doi.org/10.1007/s002990050653

Fernando, K. (1986). In Vitro propagation of mauritius pineapple. Tropical Agricul-turist.. 142:7-10.

Firoozabady, E. and Gutterson N. (2003). Cost-effective in vitro propagation methods for pineapple. Plant cell reports 21(9):844-850. DOI: https://doi.org/10.1007/s00299-003-0577-x

Hamad, A. and Taha R. (2003). The effect of hormones on tissue culture of pineapple. Jur Sains 11(1):32-37.

Hamad, A. M. and Taha R. (2008a). Effectof benzylaminopurine (BAP) on in vitro proliferation and growth of pineapple (Ananas Comosus L. Merr.) cv. Smooth cayenne. Journal of Applied Sciences 8(22):4180-4185. DOI: https://doi.org/10.3923/jas.2008.4180.4185

Hamad, A. M. and Taha R. M. (2008b). The effect of different hormones and incubation periods on in vitro proliferation of pineapple (Ananas comosus L.) Merr cv. Smooth Cayenne) shoot-tip culture. Pakistan Journal of Biology Sciences 11 (3) : 386-391. DOI: https://doi.org/10.3923/pjbs.2008.386.391

Hamad, A. M. and Taha R. M. (2008c). Effect of sequential subcultures on in vitro proliferation capacity and shoot formations pattern of pineapple (Ananas comosus L. Merr.) over different incubation periods. Scientia Horticulturae 117(4):329-334. DOI: https://doi.org/10.1016/j.scienta.2008.05.009

Hamad, A. and Taha R. (2009). Effect of explants density on the in vitro proliferation and growth of separated and cluster shoots of smooth cayenne pineapple (Ananas comosus L. Merr.). Asian Journal of Plant Sciences 8(4):313. DOI: https://doi.org/10.3923/ajps.2009.313.317

Hamad, A. M. (2017). Effect of explant length and segmentation and separated and cluster of shoots on the in vitro shoot formation and growth of pineapple (Ananas comosus L. Merr). Journal of Science and Humanities, 25: 1- 9. DOI: https://doi.org/10.37376/1571-000-025-003

Khan, S. Nasib A. and Saeed B. A. (2004). Employment of in vitro technology for large scale multiplication of pine -apples (Ananas comosos). Pakistan Journal of Botany 36(3):611-616.

Kofi, O. and Adachi T. (1993). Effect of cytokinins on the proliferation of multiple shoots of pineapple in vitro. SABRAO Journal 25(1):59-69.

Konan, E. K. Kouadio J. Y. Flori A. Durand-Gasselin T. and Rival A. (2007). Evidence for an interaction effect during in vitro rooting of oil palm (Elaeis guineensis Jacq.) somatic embryo-derived plantlets. In Vitro Cellular & Developmental Biology-Plant 43(5):456-466. DOI: https://doi.org/10.1007/s11627-007-9093-y

Murashige, T. and Skoog F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia plantarum 15(3):473-497. DOI: https://doi.org/10.1111/j.1399-3054.1962.tb08052.x

Nelson, B. J. Asare P. A. and Junior R. A. (2015). In vitro Growth and Multipli -cation of Pineapple under Different Duration of Sterilization and Different Concentrations of Benzylaminopurine and Sucrose. Biotechnology 14 (1):35. DOI: https://doi.org/10.3923/biotech.2015.35.40

Pérez, G. Yanes E. Isidrón M. and Lorenzo J. C. (2009). Phenotypic and AFLP characterization of two new pineapple somaclones derived from in vitro culture. Plant Cell, Tissue and Organ Culture 96(1):113-116. DOI: https://doi.org/10.1007/s11240-008-9463-0

Pérez, G. Yanez E. Mbogholi A. Valle B. Sagarra F. Yabor L. Aragón C. González J. Isidrón M. and Lorenzo J. C. (2012). New pineapple somaclonal variants: P3R5 and Dwarf. American Journal of Plant Sciences 3(1-11. DOI: https://doi.org/10.4236/ajps.2012.31001

Soneji, J. R. Rao P. and Mhatre M. (2002). Somaclonal variation in micropropaga -ted dormant axillary buds of pineapple (Ananas comosus L. Merr.). The Journal of Horticultural Science and Biotechnology 77(1):28-32. DOI: https://doi.org/10.1080/14620316.2002.11511452

Sripaoraya, S. Marchant R. Brian Power J. and Davey M. R. (2003). Plant regeneration by somatic embryogene -sis and organogenesis in commercial pineapple (Ananas comosus L.). In Vitro Cellular and Developmental Biology-Plant 39(5):450-454. DOI: https://doi.org/10.1079/IVP2003445

Sunitibala Devi, Y. Mujib A. and Kundu S. (1997). Efficient regenerative potential from long term culture of pineapple. Phytomorphology 47 (3) : 255-259.

Teixeira, S. L. Ribeiro J. M. and Teixeira M. T. (2006). Influence of NaClO on nutrient medium sterilization and on pineapple (Ananas comosus cv Smooth cayenne) behavior. Plant Cell, Tissue and Organ Culture 86 (3):375-378. DOI: https://doi.org/10.1007/s11240-006-9121-3

Zuraida, A. Shahnadz A. N. Harteeni A. Roowi S. Radziah C. C. and Sreera-manan S. (2011). A novel approach for rapid micropropagation of maspine pineapple (Ananas comosus L.) shoots using liquid shake culture system. African Journal of Biotechnology 10(19):3859-3866.